Hepatocytes: Methods and Protocols
Hepaytes account for approximately 80% of the liver mass and play a significant role in various aspects of liver physiopathology, exhibiting unrivaled complexity and diversity of functions. In Hepaytes: Methods and Prools, expert researchers provide the reader with methods, technical prools, and review chapters focusing on selected areas of hepayte biology including isolation, culture, differentiation and stem cells, and hepayte use in clinical, basic, and applied research. With a specific emphasis on human hepaytes, the volume presents chapters covering subjects including hepayte culture models, cryopreservation methods, differentiation assessment, liver ontogenesis, production of hepaytes from stem cells, drug/xenobiotic metabolism, toxicity and transport, bile acid and blood coagulation factor production, infection by HBV and HCV, humanized animals, biortificial liver devices, hepayte transplantation. As a volume in the highly successful Methods in Molecular Biology™ series, prool chapters include brief introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory prools, and notes on troubleshooting and avoiding known pitfalls.

Comprehensive and cutting-edge, Hepaytes: Methods and Prools will be useful to all those who are currently using or planning to use human, or animal, hepaytes to investigate any aspect of liver physiopathology or who are interested in liver development or liver stem cells and liver biotherapy.

1135369887
Hepatocytes: Methods and Protocols
Hepaytes account for approximately 80% of the liver mass and play a significant role in various aspects of liver physiopathology, exhibiting unrivaled complexity and diversity of functions. In Hepaytes: Methods and Prools, expert researchers provide the reader with methods, technical prools, and review chapters focusing on selected areas of hepayte biology including isolation, culture, differentiation and stem cells, and hepayte use in clinical, basic, and applied research. With a specific emphasis on human hepaytes, the volume presents chapters covering subjects including hepayte culture models, cryopreservation methods, differentiation assessment, liver ontogenesis, production of hepaytes from stem cells, drug/xenobiotic metabolism, toxicity and transport, bile acid and blood coagulation factor production, infection by HBV and HCV, humanized animals, biortificial liver devices, hepayte transplantation. As a volume in the highly successful Methods in Molecular Biology™ series, prool chapters include brief introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory prools, and notes on troubleshooting and avoiding known pitfalls.

Comprehensive and cutting-edge, Hepaytes: Methods and Prools will be useful to all those who are currently using or planning to use human, or animal, hepaytes to investigate any aspect of liver physiopathology or who are interested in liver development or liver stem cells and liver biotherapy.

219.99 In Stock
Hepatocytes: Methods and Protocols

Hepatocytes: Methods and Protocols

Hepatocytes: Methods and Protocols

Hepatocytes: Methods and Protocols

Overview

Hepaytes account for approximately 80% of the liver mass and play a significant role in various aspects of liver physiopathology, exhibiting unrivaled complexity and diversity of functions. In Hepaytes: Methods and Prools, expert researchers provide the reader with methods, technical prools, and review chapters focusing on selected areas of hepayte biology including isolation, culture, differentiation and stem cells, and hepayte use in clinical, basic, and applied research. With a specific emphasis on human hepaytes, the volume presents chapters covering subjects including hepayte culture models, cryopreservation methods, differentiation assessment, liver ontogenesis, production of hepaytes from stem cells, drug/xenobiotic metabolism, toxicity and transport, bile acid and blood coagulation factor production, infection by HBV and HCV, humanized animals, biortificial liver devices, hepayte transplantation. As a volume in the highly successful Methods in Molecular Biology™ series, prool chapters include brief introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory prools, and notes on troubleshooting and avoiding known pitfalls.

Comprehensive and cutting-edge, Hepaytes: Methods and Prools will be useful to all those who are currently using or planning to use human, or animal, hepaytes to investigate any aspect of liver physiopathology or who are interested in liver development or liver stem cells and liver biotherapy.

Product Details

ISBN-13: 9781607616870
Publisher: Springer-Verlag New York, LLC
Publication date: 05/07/2010
Series: Methods in Molecular Biology , #640
Edition description: 2010
Pages: 553
Product dimensions: 7.30(w) x 10.10(h) x 1.50(d)

Table of Contents

Foreword v

Preface vii

Contributors xv

1 General Review on In Vitro Hepatocyte Models and Their Applications Christiane Guguen-Guillouzo Andre Guillouzo 1

2 Human Foetal Hepatocytes: Isolation, Characterization, and Transplantation Anne Weber Thomas Touboul Sylvie Mainot Julie Branger Dominique Mahieu-Caputo 41

3 Isolation and Culture of Primary Hepatocytes from Resected Human Liver Tissue Edward L. LeCluyse Eliane Alexandre 57

4 Optimisation of the Cryopreservation of Primary Hepatocytes Nicola J. Hewitt 83

5 Cryopreservation of Human Hepatocytes for Clinical Use Ragai R. Mitry Sharon C. Lehec Robin D. Hughes 107

6 Hepatocyte Differentiation Katy M. Olsavsky Goyak Elizabeth M. Laurenzana Curtis J. Omiecinski 115

7 Reversible Manipulation of Apoptosis Sensitivity in Cultured Hepatocytes by Matrix-Mediated Manipulation of Signaling Activities Patricio Godoy Markus Schug Alexander Bauer Jan G, Hengstler 139

8 Markers and Signaling Factors for Stem Cell Differentiation to Hepatocytes: Lessons from Developmental Studies Frédéric Lemaigre 157

9 Hepatic Stem Cells Robert E. Schwartz Catherine Verfaillie 167

10 Hepatic Stem Cells and Liver Development Nalu Navarro-Alvarez Alejandro Soto-Gutierrez Naoya Kobayashi 181

11 Generation of Hepatocytes from Human Embryonic Stem Cells Neta Lavon 237

12 Isolation and Culture of Adult Human Liver Progenitor Cells: In Vitro Differentiation to Hepatocyte-Like Cells Sabine Gerbal-Chaloin Cédric Duret Edith Raulet Francis Navarro Pierre Blanc Jeanne Ramos Patrick Maurel Martine Daujat-Chavanieu 247

13 The HepaRG Cell Line: Biological Properties and Relevance as a Tool for Cell Biology, Drug Metabolism, and Virology Studies Marie-Jeanne Marion Olivier Hantz David Durantel 261

14 Transdifferentiation of Pancreatic Cells to Hepatocytes Chia-Ning Shen David Tosh 273

15 Evaluation of Drug Metabolism, Drug-Drug Interactions, and In Vitro Hepatotoxicity with Cryopreserved Human Hepatocytes Albert P. Li 281

16 The Use of Human Hepatocytes to Investigate Drug Metabolism and CYP Enzyme Induction Sylvie Klieber François Torreilles François Guillou Gérard Fabre 295

17 The Use of Hepatocytes to Investigate UDP-Glucuronosyltransferases and Sulfotransferases Sylvie Fournel-Gigleux Michael W.H. Coughtrie Mohamed Ouzzine Jacques Magdalou 309

18 The Use of Hepatocytes to Investigate Drug Uptake Transporters Kazuya Maeda Yuichi Sugiyama 327

19 Metabonomic Studies on Human Hepatocyte in Primary Culture Vincent Croixmarie Thierry Umbdenstock Olivier Cloarec Amélie Moreau Jean-Marc Pascussi Yannick Parmentier Claire Boursier-Neyret Bernard Walther 355

20 The Application of HepRG Cells in Evaluation of Cytochrome P450 Induction Properties of Drug Compounds Tommy B. Andersson 375

21 The Use of Hepatocytes to Investigate Drug Toxicity María José Gómez-Lechón José V. Castell María Teresa Donato 389

22 The Use of Human Hepatocytes to Investigate Bile Acid Synthesis Ewa C. S. Ellis Lisa-Mari Nilsson 417

23 Use of Human Hepatocytes to Investigate Blood Coagulation Factor Christine Biron-Andréani Edith Raulet Lydiane Pichard-Garcia Patrick Maurel 431

24 Use of Human Hepatocytes to Investigate HCV Infection Lydiane Pichard-Garcia Philippe Briolotti Dominique Larrey Antonio Sa-Cunha Bertrand Suc Sylvain Laporte Patrick Maurel 447

25 The Use of Hepatocytes to Investigate HDV Infection: The HDV/HepaRG Model Camille Sureau 463

26 Rodent Models of Liver Repopulation Helène Gilgenkrantz 475

27 Chimeric Mice with Humanized Liver: Tools for the Study of Drug Metabolism, Excretion, and Toxicity Stephen C. Strom Julio Davila Markus Grompe 491

28 Bioartificial Liver Support Systems Gesine Pless 511

29 Human Hepatocyte Transplantation Anil Dhawan Stephen C. Strom Etienne Sokal Ira J. Fox 525

Subject Index 535

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