Table of Contents

Introduction to Immunohishemistry. From Evolving Science to Timeless Art.- Immunohishemistry in Historical Perspective. Knowing the Past to Understand the Present.- Sample Preparation & Fixation for Histology and Pathology.- Tissue Processing.- Antigen Retrieval and Signal Amplification.- Primary Antibody Selection and Blocking Techniques for Immunohishemistry.- Probes for Fluorescent Visualization of Specific Cellular Organelles.- Enzymatic Immunohishemistry.- Fluorescent Immunohishemistry.- Double Labeling Fluorescent Immunocyhemistry.- Multiplexing and Spectral Microscopy.- In situ Hybridization (Fluorescent and Enzymatic).- Proximity Ligation Assay (PLA).- Immunohishemistry, Quality control and Principles of Validation in the Central Nervous System.- Culture and Phenotyping of Glial Cell Cultures, Gliospheres and Neurospheres.- ER+ Breast Cancer Mammosphere Formation and Analysis.- Detection of Circulating and Tissue Myeloid-Derived Suppressor Cells (MDSCs) by Flow Cytometry.- Comet Assay for the Detection of Single and Double Strand DNA Breaks.- Fluorescent-Activated Cell Sorting (Flow Cytometry).