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HPLC of Peptides and Proteins: Methods and Protocols / Edition 1

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ISBN-10:: 1617372862
ISBN-13:: 9781617372865
Pub. Date:: 11/10/2010
Publisher:: Springer-Verlag New York, LLC

ISBN-10:: 1617372862
ISBN-13:: 9781617372865
Pub. Date:: 11/10/2010
Publisher:: Springer-Verlag New York, LLC

HPLC of Peptides and Proteins: Methods and Protocols / Edition 1

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Overview

The introduction of high-performance liquid chromatography (HPLC) to the analysis of peptides and proteins some 25 years ago revolutionized the biological sciences by enabling the rapid and sensitive analysis of peptide and protein structure through the exquisite speed, sensitivity, and resolution that can be easily obtained. Today, HPLC in its various modes has become the pivotal technique in the characterization of peptides and proteins and currently plays a critical role in both our understanding of biological processes and in the development of peptide- and protein-based pharmaceuticals. The number of applications of HPLC in peptide and protein purification continues to expand at an extremely rapid rate. Solid-phase peptide synthesis and recombinant DNA techniques have allowed the production of large quantities of peptides and proteins that need to be highly purified. HPLC techniques are also used extensively in the isolation and characterization of novel proteins that will become increasingly important in the postgenomic age. The design of multidimensional purification schemes to achieve high levels of product purity further demonstrates the power of HPLC techniques not only in the characterization of cellular events, but also in the production of pepti- and protein-based therapeutics. HPLC continues to be at the heart of the analytical techniques with which scientists in both academia and in industry must arm themselves to be able to fully characterize the identity, purity, and potency of peptides and proteins.

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Product Details

ISBN-13:	9781617372865
Publisher:	Springer-Verlag New York, LLC
Publication date:	11/10/2010
Series:	Methods in Molecular Biology , #251
Edition description:	Softcover reprint of hardcover 1st ed. 2004
Pages:	414
Product dimensions:	5.90(w) x 9.00(h) x 1.00(d)

Part I. Modes of HPLC HPLC of Peptides and Proteins: Basic Theory and Methodology Marie-Isabel Aguilar Reversed-Phase High-Performance Liquid Chromatography Marie-Isabel Aguilar Ion-Exchange Chromatography Peter Stanton High-Performance Hydrophobic Interaction Chromatography Kálmán Benedek Gel Filtration Chromatography Peter Stanton Hydrophilic Interaction Chromatography Herbert Lindner and Wilfried Helliger Immobilized Metal Ion Affinity Chromatography of Proteins Michael Zachariou Immunoaffinity Chromatography of Proteins Stuart R. Gallant Liquid Chromatography-Mass Spectrometry and Tandem Mass Spectrometry of Peptides and Proteins Tun-Li Shen and Kathleen R. Noon Part II. Preparative Methodologies Capillary Separations Montserrat Carrascal and Joaquin Abian Micropreparative HPLC of Peptides and Proteins Edouard C. Nice and Marie-Isabel Aguilar Multidimensional HPLC Purification of Proteins Edouard C. Nice and Marie-Isabel Aguilar Analytical High-Performance Liquid Chromatography Kálmán Benedek Prep/Semiprep Separations of Peptides Denis B. Scanlon and James Finlayson Large-Scale Protein Chromatography Joseph Bertolini, Peter Gomme, and Patrick Thomas Part III. Applications Proteolytic Peptide Mapping, Peter Højrup Mass Spectrometric Characterization of Posttranslationally Modified Proteins-Phosphorylation Martin R. Larsen Analyses of Glycopeptides and Glycoproteins by Liquid Chromatography-Mass Spectrometry and Liquid Chromatography-Tandem Mass Spectrometry Nana Kawasaki, Miyako Ohta, Satsuki Itoh, and Takao Hayakawa HPLC in the Analysis of Peptide Metabolism Rebecca A. Lew Isolation and Characterization of Naturally ProcessedMHC-Bound Peptides From the Surface of Antigen-Presenting Cells Anthony W. Purcell Australian Funnel-Web Spider Venom Analyzed With On-Line RP-HPLC Techniques David Wilson and Paul Alewood HPLC and Mass Spectrometry of Intrinsic Membrane Proteins Julian P. Whitelegge IgG Purification Maree S. Powell and Bruce D. Wines DNA-Binding Proteins: LC-MS to Identify Key Domains in RNA Polymerase-Promoter Interactions Malcolm Buckle Sensitive Enzymatic Analysis of Histidine Decarboxylase Using HPLC Jordi Gómez-Ramirez, Isaac Blanco, and Jordi Ortiz Automated vs Manual Profiling of Peptide Libraries by Mass Spectrometry Jean-Louis Aubagnac, Christine Enjalbal, Jean Martinez, Pierre Sanchez, and Gilles Subra Proteome Analysis Matthew J. Powell and Aaron T. Timperman

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