Table of Contents
Contributors
Author Biographies
Preface to the Fifth EditionSection I General Topics in Clinical Virology
1 The Taxonomy, Classification, and Characterization of Medically Important Viruses 3Steven J. Drews
2 Quality Assurance and Quality Control in Clinical and Molecular Virology 27Matthew J. Bankowski
3 Regulatory Compliance 35Linoj Samuel
4 Laboratory Safety 41K. Sue Kehl
5 Laboratory Design 51Matthew J. Binnicker
Section II Laboratory Procedures For Detecting Viruses
6 Specimen Requirements Selection, Collection, Transport, and Processing 59Reeti Khare and Thomas E. Grys
7 Primary Isolation of Viruses 79Marie L. Landry and Diane Leland
8 Viral Antigen Detection 95Diane S. Leland and Ryan F. Relich
9 Serologic (Antibody Detection) Methods 105Dongxiang Xia, Debra A. Wadford, Christopher P. Preas, and David P. Schnurr
10 Nucleic Acid Extraction in Diagnostic Virology 117Raymond H. Widen and Suzane Silbert
11 Nucleic Acid Amplification by Polymerase Chain Reaction 129Ana María Cárdenas and Kevin Alby
12 Isothermal Nucleic Acid Amplification Methods 137Harald H. Kessler and Evelyn Stelzl
13 Quantitative Molecular Methods 145Natalie N. Whitfield and Donna M. Wolk
14 Signal Amplification Methods 167Yun (Wayne) Wang
15 DNA Sequencing for Clinical and Public Health Virology: Some Assembly Required 173Joanne Bartkus
16 Phenotypic and Genotypic Antiviral Susceptibility Testing 201Martha T. Van Der Beek and Eric C. J. Claas
17 Point-of-Care Diagnostic Virology 229James J. Dunn and Lakshmi Chandramohan
18 Future Technology 243Erin Mcelvania Tekippe and Carey-Ann D. Burnham
Section III Viral Pathogens
19 Respiratory Viruses 257Christine Robinson, Michael J. Loeffelholz, and Benjamin A. Pinsky
20 Enteroviruses and Parechoviruses 277M. Steven Oberste and Mark A. Pallansch
21 Measles, Mumps, and Rubella Viruses 293William J. Bellini, Joseph P. Icenogle, and Carole J. Hickman
22 Gastrointestinal Viruses 311Michael D. Bowen
23 Hepatitis A and E Viruses 329Gilberto Vaughan and Michael A. Purdy
24 Hepatitis B and D Viruses 341Rebecca T. Horvat
25 Hepatitis C Virus 351
Melanie Mallory and David Hillyard
26 Herpes Simplex Viruses and Varicella Zoster Virus 363Scott H. James and Mark N. Prichard
27 Cytomegalovirus 373Preeti Pancholi and Stanley I. Martin
28 Epstein-Barr Virus 387Derrick Chen and Belinda Yen-Lieberman
29 Human Herpesviruses 6, 7, and 8 399Sheila C. Dollard and Timothy M. Karnauchow
30 Human Papillomaviruses 413Susan Novak-Weekley and Robert Pretorius
31 Human Polyomaviruses 427Rebecca J. Rockett, Michael D. Nissen, Theo P. Sloots, and Seweryn Bialasiewicz
32 Parvoviruses 443Richard S. Buller
33 Poxviruses 457Ashley V. Kondas and Victoria A. Olson
34 Rabies Virus 473Robert J. Rudd and April A. Davis
35 Arboviruses 493Laura D. Kramer, Elizabeth B. Kauffman, Norma P. Tavakoli
36 Animal-Borne Viruses 515Gregory J. Berry, Michael J. Loeffelholz, and Gustavo Palacios
37 Human Immunodeficiency Viruses and Human T-lymphotropic Viruses 527Jörg Schüpbach
38 Chlamydiae 545Barbaravan Der Pol and Charlotte A. Gaydos
39 The Human Virome 561Matthew C. Ross, Nadim J. Ajami, and Joseph F. Petrosino
40 Human Susceptibility and Response to Viral Diseases 567Ville Peltola and Jorma Ilonen
Appendixes: Reference Virology Laboratories
Appendix 1 Reference Virology Laboratory Testing Performed at the Centers for Disease Control 581Roberta B. Carey
Appendix 2 Public Health Laboratory Virology Services 585Jane Getchell
Appendix 3 International Reference Laboratories Offering Virology Services 595Ariel I. Suarez and Cristina Videla
Index 607