This volume contains the papers presented at the 7th International Conference on Independent Component Analysis (ICA) and Source Separation held in L- don, 9–12 September 2007, at Queen Mary, University of London. Independent Component Analysis and Signal Separation is one of the most exciting current areas of research in statistical signal processing and unsup- vised machine learning. The area has received attention from several research communities including machine learning, neural networks, statistical signal processing and Bayesian modeling. Independent Component Analysis and Signal Separation has applications at the intersection of many science and engineering disciplinesconcernedwithunderstandingandextractingusefulinformationfrom data as diverse as neuronal activity and brain images, bioinformatics, com- nications, the World Wide Web, audio, video, sensor signals, or time series. This year’s event was organized by the EPSRC-funded UK ICA Research Network (www.icarn.org). There was also a minor change to the conference title this year with the exclusion of the word‘blind’. The motivation for this was the increasing number of interesting submissions using non-blind or semi-blind techniques that did not really warrant this label. Evidence of the continued interest in the field was demonstrated by the healthy number of submissions received, and of the 149 papers submitted just over two thirds were accepted.