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Vascular Biology Protocols / Edition 1

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ISBN-10:: 1588295745
ISBN-13:: 9781588295743
Pub. Date:: 07/25/2008
Publisher:: Springer-Verlag New York, LLC

ISBN-10:: 1588295745
ISBN-13:: 9781588295743
Pub. Date:: 07/25/2008
Publisher:: Springer-Verlag New York, LLC

Vascular Biology Protocols / Edition 1

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Overview

Over the past decades, the pathogenesis, diagnosis, treatment and prevention of cardiovascular diseases have been benefited significantly from intensive research activities. In order to provide a comprehensive “manual” in a field that has become as broad and deep as cardiovascular medicine, this volume of “Methods in Molecular Medicine” covers a wide spectrum of in vivo and in vitro techniques encompassing biochemical, pharmacological and molecular biology disciplines which are currently used to assess vascular disease progression. Each chapter included in this volume focuses on a specific vascular biology technique and describes various applications as well as caveats of these techniques. The prools included here are described in detail, allowing beginners with little experience in the field of vascular biology to embark on new research projects.

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Product Details

ISBN-13:	9781588295743
Publisher:	Springer-Verlag New York, LLC
Publication date:	07/25/2008
Series:	Methods in Molecular Medicine , #139
Edition description:	2007
Pages:	372
Product dimensions:	5.98(w) x 9.02(h) x 0.03(d)

Rat Carotid Artery Balloon Injury Model.- Histological and Morphometric Analyses for Rat Carotid Balloon Injury Model.- Plaque Rupture Model in Mice.- Immunostaining of Mouse Atherosclerotic Lesions.- Surgical Animal Model of Ventricular Hypertrophy.- Animal Models of Hypertension.- Rat Models of Cardiac Insulin Resistance.- The Isolated, Perfused Pseudo-Working Heart Model.- Altering and Analyzing Glucose Metabolism in Perfused Hearts of Transgenic Mice.- Methods in the Evaluation of Cardiovascular Renin Angiotensin Aldosterone Activation and Oxidative Stress.- Detection and Quantification of Apoptosis in the Vasculature.- Endothelial Progenitor Cell and Mesenchymal Stem Cell Isolation, Characterization, Viral Transduction.- Assessment of Endothelial Damage/Dysfunction: A Focus on Circulating Endothelial Cells.- Evaluation of Endothelial Function by In Vivo Microscopy.- Haptotaxis of Endothelial Cell Migration Under Flow.- Isolation, Culture, and Functional Analysis of Adult Mouse Cardiomyocytes.- Mechanical Measurement of Contractile Function of Isolated Ventricular Myocytes.- Studying Ischemia and Reperfusion in Isolated Neonatal Rat Ventricular Myocytes Using Coverslip Hypoxia.- Isolation and Functional Studies of Rat Aortic Smooth Muscle Cells.- Detection of Reactive Oxygen Species and Nitric Oxide in Vascular Cells and Tissues.- Assessment of Protein Glycoxidation in Ventricular Tissues.- Assessment of PI-3 Kinase and Akt in Ischemic Heart Diseases in Diabetes.- Two-Dimensional Gel Electrophoresis in Platelet Proteomics Research.- Stem Cell Therapy in the Heart and Vasculature.

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Editorial Reviews

Reviewer: Vera P. Shively, BS, MS (Northwestern University Feinberg School of Medicine)
Description: This book of detailed protocols for researchers in the field of vascular biology is particularly relevant for scientists using animal and cell culture models.
Purpose: The purpose is to provide a comprehensive manual for in vivo and in vitro techniques commonly used to study the progression of cardiovascular disease. A book such as this one was needed and it meets the authors' objectives.
Audience: Researchers new to the techniques commonly used to study cardiovascular disease, including clinician scientists, basic research scientists, graduate students, or residents are the intended audience. Although the book is written for beginners, even experienced researchers would find techniques that are new to them.
Features: The book includes protocols for animal models, ex vivo organ systems and relevant functional studies, cell culture isolations, and pertinent experiments. In addition, it presents protocols for basic as well as sophisticated histology, along with instructions for the assessment of results. The final chapter includes methodology for stem cell therapy. For researchers in vascular biology, the strength of this book is the relevance of the protocols and the detail in which they are described. The sections on materials and methods are the most comprehensive I've seen, including separate lists for solutions, supplies, and surgical tools, as well as extensive notes and references on the nuances of each technique. The protocols for animal surgery are particularly thorough. One of the shortcomings of the book is paradoxically one of its assets — the "see Notes" feature. Basically, it is an example of the limitations, and sometimes awkwardness, of print in the age of electronic text with links. The "see Notes" feature augments each chapter with relevant information, but it is a bit of a nuisance to flip back and forth through the pages.
Assessment: This would be a valuable addition to any laboratory doing research in vascular biology. It is small in size, but substantial in its usefulness to researchers in this area of study. It is not a replacement for the Current Protocols series from John Wiley & Sons, but could be considered a complement to this series as an initial resource for protocols with a narrower focus.

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From the reviews:

"This book of detailed prools for researchers in the field of vascular biology is particularly relevant for scientists using animal and cell culture models. … Researchers new to the techniques commonly used to study cardiovascular disease, including clinician scientists, basic research scientists, graduate students, or residents are the intended audience. … This would be a valuable addition to any laboratory doing research in vascular biology." (Vera P. Shively, Doody’s Review Service, December, 2008)

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