Table of Contents
Preface ix
Section I Structures & Functions of Proteins & Enzymes 1
1 Biochemistry & Medicine Victor W. Rodweli, PhD 1
2 Water & pH Peter J. Kennelly, PhD Victor W. Rodweli, PhD 6
3 Amino Acids & Peptides Peter J. Kennelly, PhD Victor W. Rodweli, PhD 15
4 Proteins: Determination of Primary Structure Peter J. Kennelly, PhD Victor W. Rodweli, PhD 24
5 Proteins: Higher Orders of Structure Peter J. Kennelly, PhD Victor W. Rodweli, PhD 34
Section II Enzymes: Kinetics, Mechanism, Regulation, & Role of Transition Metals 49
6 Proteins: Myoglobin & Hemoglobin Peter J. Kennelly, PhD Victor W. Rodwell, PhD 49
7 Enzymes: Mechanism of Action Peter J. Kennelly, PhD Victor W. Rodweli, PhD 59
8 Enzymes: Kinetics Victor W. Rodweli, PhD 71
9 Enzymes: Regulation of Activities Peter J. Kennelly, PhD Victor W. Rodweli, PhD 85
10 The Biochemical Roles of Transition Metals Peter J. Kennelly, PhD 96
Section III Bioenergetics 109
11 Bioenergetics: The Role of ATP Kathleen M. Botham, PhD, DSc Peter A. Mayes, PhD, DSc 109
12 Biologic Oxidation Kathleen M. Botham, PhD, DSc Peter A. Mayes, PhD, DSc 115
13 The Respiratory Chain & Oxidative Phosphorylation Kathleen M. Botham, PhD, DSc Peter A. Mayes, PhD, DSc 121
Section IV Metabolism of Carbohydrates 133
14 Overview of Metabolism & the Provision of Metabolic Fuels Owen P. McGuinness, PhD 133
15 Saccharides (ie. Carbohydrates) of Physiological Significance Owen P. McGuinness, PhD 147
16 The Citric Acid Cycle: A Pathway Central to Carbohydrate, Lipid, & Amino Acid Metabolism Owen P. McGuinness, PhD 156
17 Glycolysis & the Oxidation of Pyruvate Owen P. McGuinness, PhD 163
18 Metabolism of Glycogen Owen P. McGuinness, PhD 171
19 Gluconeogenesis & the Control of Blood Glucose Owen P. McGuinness, PhD 180
20 The Pentose Phosphate Pathway & Other Pathways of Hexose Metabolism Owen P. McGuinness, PhD 191
Section V Metabolism of Lipids 205
21 Lipids of Physiologic Significance Kathleen M. Botham, PhD, DSc Peter A. Mayes, PhD, DSc 205
22 Oxidation of Fatty Acids: Ketogenesis Kathleen M. Botham, PhD, DSc Peter A. Mayes, PhD, DSc 217
23 Biosynthesis of Fatty Acids & Eicosanoids Kathleen M. Botham, PhD, DSc Peter A. Mayes, PhD, DSc 226
24 Metabolism of Acylglycerols & Sphingolipids Kathleen M. Botham, PhD, DSc Peter A. Mayes, PhD, DSc 239
25 Lipid Transports Storage Kathleen M. Botham, PhD, DSc Peter A. Mayes, PhD, DSc 247
26 Cholesterol Synthesis, Transport, & Excretion Kathleen M. Botham, PhD, DSc Peter A. Mayes, PhD, DSc 259
Section VI Metabolism of Proteins & Amino Acids 273
27 Biosynthesis of the Nutritionally Nonessential Amino Acids Victor W. Rodwell, PhD 273
28 Catabolism of Proteins & of Amino Acid Nitrogen Victor W. Rodwell, PhD 279
29 Catabolism of the Carbon Skeletons of Amino Acids Victor W. Rodwell, PhD 290
30 Conversion of Amino Acids to Specialized Products Victor W. Rodwell, PhD 306
31 Porphyrins & Bile Pigments Victor W. Rodwell, PhD 315
Section VII Structure, Function, & Replication of Informational Macromolecules 329
32 Nucleotides Victor W. Rodwell, PhD 329
33 Metabolism of Purine & Pyrimidine Nucleotides Victor W. Rodwell, PhD 337
34 Nucleic Acid Structures Function P. Anthony Weil, PhD 348
35 DNA Organization, Replication, & Repair P. Anthony Weil, PhD 360
36 RNA Synthesis, Processing, & Modification P. Anthony Weil, PhD 384
37 Protein Synthesis & the Genetic Code P. Anthony Weil, PhD 404
38 Regulation of Gene Expression P. Anthony Weil, PhD 420
39 Molecular Genetics, Recombinant DNA, & Genomic Technology P. Anthony Weil, PhD 444
Section VIII Biochemistry of Extracellular & Intracellular Communication 467
40 Membranes: Structure & Function P. Anthony Weil, PhD 467
41 The Diversity of the Endocrine System P. Anthony Weil, PhD 488
42 Hormone Action & Signal Transduction P. Anthony Weil, PhD 508
Section IX Special Topics (A) 527
43 Nutrition, Digestion, & Absorption David A. Bender, PhD 527
44 Micronutrients: Vitamins & Minerals David A. Bender, PhD 535
45 Free Radicals & Antioxidant Nutrients David A. Bender, PhD 549
46 Glycoproteins David A. Bender, PhD 554
47 Metabolism of Xenobiotics David A. Bender, PhD Robert K. Murray, MD, PhD 564
48 Clinical Biochemistry David A. Bender, PhD 568
Section X Special Topics (B) 581
49 Intracellular Traffic & Sorting of Proteins Kathleen M. Botham, PhD, DSc Robert K. Murray, MD, PhD 581
50 The Extracellular Matrix Kathleen M. Botham, PhD, DSc Robert K. Murray, MD, PhD 599
51 Muscle & the Cytoskeleton January D. Haile, PhD Peter J. Kennelly, PhD 618
52 Plasma Proteins & Immunoglobulins Peter J. Kennelly, PhD 634
53 Red Blood Cells Peter J. Kennelly, PhD 653
54 White Blood Cells Peter J. Kennelly, PhD 664
Section XI Special Topics (C) 677
55 Hemostasis&Thrombosis Peter L. Gross, MD, MSc, FRCP(C) P. Anthony Weil, PhD Margaret L. Rand, PhD 677
56 Cancer: An Overview Molly Jacob, MD, PhD, MNASc, FRCPath Joe Varghese, MD, PhD P. Anthony Weil, PhD 689
57 The Biochemistry of Aging Peter J. Kennelly, PhD 717
58 Biochemical Case Histories David A. Bender, PhD 729
The Answer Bank 741
Index 745