Table of Contents

1. Methods for assessing T cell receptor recognition; B. M. Baker 2. Energetic approaches to the characterization of the E. coli PriA helicase; W. M. Bujalowski 3. Practical approaches to protein folding and assembly: Spectroscopic strategies in thermodynamics and kinetics C. Clark, J. Walters, S. L. Milam 4. Using thermodynamics to understand progesterone receptor function: Method and theory; D. Bain, K. Connaghan-Jones, A. Moody 5. Measurement of Mg2+ - RNA interaction free energies by use of a fluorescent indicator dye; D. Draper, D. Grilley, A. M. Soto 6. Analysis of repeat-protein folding using nearest-neighbor statistical mechanical models; D. Barrick 7.Methods for assessing folding and binding energetics in proteins; E. Freire, A. Schon, A. Velazquez-Campoy 8. Kinetic equilibrium analysis of the myosin ATPase; E. M. De La Cruz, E. M. Ostap 9. Methodological approaches to assessing cooperativity using the Hill Coefficient; J. M. Holt, G. Ackers 10. Experimental evaluation of the forces that define membrane protein structure; L. Tamm, J. U. Bowie, H. Hong, N. HyunJoong Joh 11. Energetic Approaches to DNA-Drug Binding Interactions; E. Lewis, W. D. Wilson, J. T. Petty 12. NMR analysis of dynein light chain phosphorylation and interactions with diverse ligands; E. Barbar, G. Benison 13. Cooperative DNA binding of the DNA-repair protein AGT; M. G. Fried 14. Measurement of Parvalbumin Divalent Ion Affinity by Isothermal Titration Calorimetry; M. T. Henzl 15. Methodological approaches to the study of conformational fluctuations; V. J. Hilser 16. Applications of molecular dynamics simulations to study protein quaternary structure changes involved in allostery; J. Carey, R. Ettrich 17. Thermodynamics of protein DNA binding with hyperthermophile proteins; J. Shriver, S. Edmondson 18. Experimental approaches to the study of protein dynamics; J. Wand 19. Methodological approaches to the thermodynamics of cholesterol-lipid interactions; J. Huang, G. W. Geigenson 10. Methods applied to the analysis of allostery and cooperativity in calmodulin; M. A. Shea 21. Analysis of DNA binding by footprinting methods; M. Brenowitz 22. RNA function from thermodynamic cycles: from the conceptual to the practical; P. Bevilacqua, N. Siegfried 23. Biophysical techniques applied to virus assembly and stability; A. Zlotnick 24. Bioenergetic methods applied to allostery; R. Noble 25. The effect of kinetics on sedimentation velocity profiles: the role of intermediates; J. J. Correia, W. Stafford 26. Methods for the bioenergetic characterization of bacteriorhodopsin; G. J. Turner 27. Ultracentrifugation methods applied to bioenergetics of contractile proteins; W. Stafford