A Comparison Of The Neuroprotective Effects Of Propofol, Etomidate, And Thiopental On Hydrogen Peroxide Induced Cellular Injury Of Pc 12 Cells.

Overview

There is considerable risk of cerebral ischemia in patients who undergo neurosurgical, cardiac, and vascular surgery. Given the large population of patients undergoing these types of surgeries, the "at-risk" population is significantly increasing, leading to an increased interest in identifying anesthetic agents that might decrease the brain's susceptibility to ischemia or neuronal injury.1 Cell death can occur via one of two different mechanisms, necrosis or apoptosis. Propofol, etomidate, and thiopental are ...
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Overview

There is considerable risk of cerebral ischemia in patients who undergo neurosurgical, cardiac, and vascular surgery. Given the large population of patients undergoing these types of surgeries, the "at-risk" population is significantly increasing, leading to an increased interest in identifying anesthetic agents that might decrease the brain's susceptibility to ischemia or neuronal injury.1 Cell death can occur via one of two different mechanisms, necrosis or apoptosis. Propofol, etomidate, and thiopental are three of the most commonly used anesthetic agents thought to contain properties that inhibit cellular necrosis or apoptosis. Determining which agent provides the best inhibition of cell death will lead to better patient outcomes and prevent further neurological complications. The purpose of this study was to compare the neuroprotective effects of propofol, etomidate, and thiopental using rat pheochromocytoma cell line (PC12), analogous to human neuronal tissue.1,2 Cultured PC12 cells were treated with 300uM H2O2 followed by varying doses of propofol, etomidate, and thiopental and were incubated for four hours. Visual evaluation, cell viability assays, lactic dehydrogenase assays, Lowry protein studies, and caspase assays were then performed to assess neuroprotection. Statistical analysis was performed using Tukey's multiple comparison test, ANOVA, confidence interval of 95%, and P value< 0.05. PC 12 cells injured through exposure to H2O2, then treated with propofol, etomidate, and thiopental exhibited different effects of neuroprotection. Propofol exhibited a six-fold decrease in lactate dehydrogenase release from cell tissue compared to those not treated with propofol. Even more, PC 12 cells injured through exposure to H2O2, then treated with propofol did not show a decrease in caspase activity. Etomidate and thiopental exhibited no effect of neuroprotection at varying doses of 100-1000microM. In conclusion, only propofol demonstrated a significant neuroprotective effect, which may be due to the inhibition of cellular necrosis rather than the inhibition of apoptosis. (Key Words: propofol, etomidate, thiopental, neuroprotection, lactate dehydrogenase, caspase, necrosis, apoptosis, PC 12 cells)
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Product Details

  • ISBN-13: 9781243454300
  • Publisher: BiblioLabsII
  • Publication date: 9/2/2011
  • Pages: 96
  • Product dimensions: 7.44 (w) x 9.69 (h) x 0.20 (d)

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