A Practical Guide to Protein and Peptide Purification for Microsequencing

by Paul T. Matsudaira (Editor)

Overview

Why a Second Edition?The Second Edition provides practical answers to the general question, "How can I obtain useful sequence information from my protein or peptide?" rather than the more specific question asked in the first edition, "How can I obtain the N-terminal sequence?" Important new methods include ways of dealing with blocked N termini, computer analysis of protein sequences, and the recent revolution in mass spectrometry. - Mass spectrophotometric characterization of proteins and peptides - N-terminal sequencing of proteins with blocked N termini - Internal amino acid sequence analysis after protease digestion in-gel and on-blot - Improved microscale peptide purification methods - Computer analysis of protein sequences - New protocols tested and refined through everyday use in authors' laboratories - Updated reference chapter covering all aspects of protein microsequencing

Product Details

ISBN-13:	9780080924618
Publisher:	Elsevier Science & Technology Books
Publication date:	12/02/2012
Sold by:	Barnes & Noble
Format:	eBook
Pages:	184
File size:	4 MB

Table of Contents

Strategies for Obtaining Partial Amino Acid Sequence Data from Small Quantities (5nmol) of Pure of Partially Purified Protein. Enzymatic Digestion of Proteins and HPLC Peptide IsolationPurification of Proteins and Peptides By SDS-PAGEInternal Amino Acid Sequence Analysis of Proteins After in Situ Protease Digestion on NitrocelluloseMass Spectrometric Strategies for the Structural Characterization of Proteins

Editorial Reviews

The second edition reflects continuous refinements at the cutting edge of protein purification techniques to obtain not only N-terminal sequence information but also internal sequence information. Provides lab protocols for those asking the question, "How can I obtain useful sequence information from my protein or peptide?"--rather than the more general first edition question, "How can I obtain the N-terminal sequence?" Plastic comb binding. Annotation c. Book News, Inc., Portland, OR (booknews.com)

Booknews

Reviews of the First Edition:
"Like the evening network television news programs, this book gives a useful summary of the highlights (of protein chemistry) to enable the masses to go forth armed with enough information to survive in the world." —Cell

From the Publisher