A Practical Guide to the Study of Calcium in Living Cells

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Overview

A Practical Guide to the Study of Calcium in Living Cells describes popular techniques along with helpful do's and don't's and computer programs. The volume enables investigators to evaluate confocal images, use the latest dyes, and design Calcium buffers appropriate to their research needs. This book is designed for laboratory use by graduate students, technicians, and researchers in many disciplines, ranging from molecular to cellular levels of investigation.

Key Features
* Describes techniques for detection of [Ca2+]I: Ca2+ - sensitive microelectrodes
* Fluorescent dyes
* Luminescent proteins
* Includes techniques for perturbing intracellular Ca2+
* Covers detailed methodology plus problems and pitfalls of each technique
* Contains a practical guide to preparing Ca2+ buffers with an easy-to-use computer program
* Color plates illustrate techniques such as
* Confocal ratio-imaging
* Use of aequorin

Audience: Researchers, graduate students, and technicians in cell, developmental, and molecular biology, neuroscience, physiology, biochemistry, biophysics, and optical sciences.

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Editorial Reviews

From the Publisher
"This multi-authored book... provides theoretical and applied aspects of different established techniques for the measurement of [Ca2+]i at submicromolar concentration. Each chapter is organized, keeping in view the needs of a person working in the laboratory, by giving the detailed methodology required for the implementation of that particular technique, and all the chapters are well referenced... The book provides a pragmatic approach for the estimation of cytosolic [Ca2+]i and also provides a means for studying the spatial and temporal resolution of [Ca2+]i. The book achieves the stated goal in the preface, 'this material will be very useful to the investigator who wants to apply any of these methods to his or her research.' It is a very well-written monograph and includes useful illustrations and helpful, practical points."
--RAMESH BHALLA, University of Iowa, Iowa City
"The book achieves the stated goal in the preface, this material will be very useful to the investigator who wants to apply any of these methods to his or her research. It is a very well-written monograph and includes useful illustrations and helpful, practical points."
--MOLECULAR & CELLULAR NEUROSCIENCES
Alvin Telser
This book is a detailed practical guide to the use and study of calcium in living cells. The book is volume 40 in a series that has been published for the past 30 years. "The major purpose is to provide a complete practical guide to the study of calcium in living cells. It covers the preparation of calcium-containing buffers, state-of-the art measurement of free calcium, and the most sensitive techniques for measuring calcium by sophisticated optical techniques. These are very worthy objectives, and the book readily fulfills its intended mission. "This volume is intended for any researcher from graduate student to established investigator who wishes to do detailed studies of the role of calcium in living cells. The book can be easily used by any and all of these individuals. Each contributor is a recognized expert in the field at large or in one of the specialized subdivisions of the field. "Most of the illustrations are graphs or diagrams; they are of high quality and are easy to read and interpret. There is a small number of color plates and halftone micrographs, also of high quality. There are many appropriate references and many that are very current. The table of contents and index are also very useful. One of the contributors offers a computerized spreadsheet program, for a nominal fee, that would be very helpful to the novice for doing the complex calculations described in that specific chapter. "This is an excellent reference volume to have in any biomedical or life sciences library. It would also be a valuable addition to any laboratory in this important field.
From The Critics
Reviewer: Alvin Telser, PhD (Northwestern University Feinberg School of Medicine)
Description: This book is a detailed practical guide to the use and study of calcium in living cells. The book is volume 40 in a series that has been published for the past 30 years.
Purpose: The major purpose is to provide a complete practical guide to the study of calcium in living cells. It covers the preparation of calcium-containing buffers, state-of-the art measurement of free calcium, and the most sensitive techniques for measuring calcium by sophisticated optical techniques. These are very worthy objectives, and the book readily fulfills its intended mission.
Audience: This volume is intended for any researcher from graduate student to established investigator who wishes to do detailed studies of the role of calcium in living cells. The book can be easily used by any and all of these individuals. Each contributor is a recognized expert in the field at large or in one of the specialized subdivisions of the field.
Features: Most of the illustrations are graphs or diagrams; they are of high quality and are easy to read and interpret. There is a small number of color plates and halftone micrographs, also of high quality. There are many appropriate references and many that are very current. The table of contents and index are also very useful. One of the contributors offers a computerized spreadsheet program, for a nominal fee, that would be very helpful to the novice for doing the complex calculations described in that specific chapter.
Assessment: This is an excellent reference volume to have in any biomedical or life sciences library. It would also be a valuable addition to any laboratory in this important field.

4 Stars! from Doody
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Product Details

  • ISBN-13: 9780125641418
  • Publisher: Elsevier Science
  • Publication date: 3/10/1994
  • Series: Methods in Cell Biology Series
  • Pages: 368
  • Product dimensions: 7.50 (w) x 9.40 (h) x 0.90 (d)

Table of Contents

Ca2+ Buffers and [Ca2+]I Perturbation Techniques:
D.M. Bers, C.W. Patton, and R. Nuccitelli, A Practical Guide to the Preparation of Ca2+ Buffers.
R. Zucker, Photorelease Techniques for Raising or Lowering Intracellular Ca2+.
D. Thomas and M.R. Hanley, Pharmacological Tools for Perturbing Intracellular Calcium Storage.
Microelectrode Techniques for Measuring [Ca2+]I and Ca2+ Fluxes:
S. Baudet, L. Hove-Madsen, and D.M. Bers, How to Make and to Use Calcium-Specific Mini- and Microelectrodes.
P.J.S. Smith, R.H. Sanger, and L.F. Jaffe, The Vibrating Ca2+ Electrode: A New Technique for Detecting Plasma Membrane Regions of Ca2+ Influx and Efflux.
C.A. Leech and G.G. Holz, IV, Application of Patch Clamp Methods to the Study of Calcium Currents and Calcium Channels.
Fluorescence Techniques for Imaging [Ca2+]I:
J.P.Y. Kao, Practical Aspects of Measuring [Ca2+] with Fluorescent Indicators.
S.J. Morris, T.B. Wiegmann, L.W. Welling, and B.M. Chronwall, Rapid Simultaneous Estimation of Intracellular Calcium and pH.
T.J. Keating and R.J. Cork, Improved Spatial Resolution in Ratio Images Using Computational Confocal Techniques.
P.A. Diliberto, X.F. Wang, and B. Herman, Confocal Imaging of Ca2+ in Cells.
S. Girard and D.E. Clapham, Simultaneous Near Ultraviolet and Visible Excitation Confocal Microscopy of Calcium Transients in Xenopus Oocytes.
Use of Aequorin for [Ca2+]I Imaging:
K.R. Robinson, T.J. Keating, and R. J. Cork, Inexpensive Techniques for Measuring [Ca2+]I Changes Using a Photomultiplier Tube.
A.L. Miller, E. Karplus, and L.F. Jaffe, Imaging [Ca2+]I with Aequorin Using a Photon Imaging Detector.
R. Rizzuto, M. Brini, and T. Pozzan, Targeting Recombinant Aequorin to Specific Intracellular Organelles.
Index.
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