Adenovirus Methods and Protocols: Volume 2: Ad Proteins and RNA, Lifecycle and Host Interactions, and Phyologenetics / Edition 2

Adenovirus Methods and Protocols: Volume 2: Ad Proteins and RNA, Lifecycle and Host Interactions, and Phyologenetics / Edition 2

by William S. M. Wold
     
 

ISBN-10: 1617378356

ISBN-13: 9781617378355

Pub. Date: 11/10/2010

Publisher: Springer-Verlag New York, LLC

Adenovirus Methods and Protocols, Second Edition, now in two volumes, is an essential resource for adenovirus (Ad) researchers beginning in the field, and an inspirational starting point for researchers looking to branch into new areas of Ad study. In addition to updating and expanding important chapters from the first edition, the authors have added new chapters

Overview

Adenovirus Methods and Protocols, Second Edition, now in two volumes, is an essential resource for adenovirus (Ad) researchers beginning in the field, and an inspirational starting point for researchers looking to branch into new areas of Ad study. In addition to updating and expanding important chapters from the first edition, the authors have added new chapters that address innovative, exciting areas of emphasis in Ad research, including Ad vector construction and use, real-time PCR, use of new animal models, and methods for quantification of Ad virus or virus expression/interactions. The protocols presented are written by trendsetting researchers.

Product Details

ISBN-13:
9781617378355
Publisher:
Springer-Verlag New York, LLC
Publication date:
11/10/2010
Series:
Methods in Molecular Medicine Series, #131
Edition description:
Softcover reprint of hardcover 2nd ed. 2007
Pages:
362
Product dimensions:
5.98(w) x 9.02(h) x 0.03(d)

Table of Contents

Ann E. Tollefson, Mohan Kuppuswamy, and William S. M. Wold

TABLE OF CONTENTS

Vol. II Topics: Ad proteins and RNA, lifecycle and host interactions, and phylogenetics

1. Analysis of the Efficiency of Adenovirus Transcription

Cristina Iftode and S. J. Flint

2. The Use of In Vitro Transcription to Probe Regulatory Functions of Viral Protein Domains

Paul M. Loewenstein, Chao-Zhong Song and Maurice Green

3. Preparation of Soluble Extracts from Adenovirus Infected Cells for Studies of RNA Splicing

Oliver Mühlemann and Göran Akusjärvi

4. In Vitro Methods to Study RNA Interference During an Adenovirus Infection

Gunnar Andersson, Ning Xu and Göran Akusjärvi

5. Simultaneous Detection of Adenovirus RNA and Cellular Proteins by Fluorescent Labeling In Situ

Eileen Bridge

6. Study of Nucleolar Localization of Adenovirus Core Proteins

David A. Matthews

7. Analysis of Adenovirus Infections in Synchronized Cells

David A. Ornelles, Robin N. Shepard and Felicia D. Goodrum

8. Co-Immunoprecipitation of Protein Complexes

Peter Yaciuk

9. Chromatin Immunoprecipitation (ChIP) to Study the Binding of Proteins to the Adenovirus Genome in Vivo

Jihong Yang and Patrick Hearing

10. Assaying Protein-DNA Interactions In Vivo and In Vitro Using Chromatin Immunoprecipitation and Electrophoretic Mobility Shift Assays

Pilar Perez-Romero and Michael J. Imperiale

11. Identifying Functional Adenovirus-Host Interactions Using Tandem Mass Spectrometry

Anuj Gaggar, Dmitry Shayakhmetov, and Andre Lieber

12. The Use of Cell Microinjection for the in vivo Analysis of Viral Transcriptional Regulatory Protein Domains

Maurice Green, Andrew Thorburn, Robert Kern and Paul M. Loewenstein

13. Determination of the Transforming Activities of Adenovirus Oncogenes

Michael Nevels and Thomas Dobner

14. Human Adenovirus Type 12: Crossing Species Barriers to Immortalize the Viral Genome

Walter Doerfler

15. Measurement of NK Cell Lytic Activity of Adenovirus-Infected or Adenovirus-Transformed Cells

John M. Routes

16. A Flow Cytometric Assay for Analysis of NK Cell-Mediated Cytolysis of Adenovirus-Transformed Cells

Graham Bottley, Graham P. Cook, and G. Eric Blair

17. Large Scale Purification and Crystallization of Adenovirus Hexon

John J. Rux and Roger M. Burnett

18. Synthesis and Assay of Recombinant Adenovirus Protease

Joseph M. Weber

19. Assay for the Adenovirus Proteinase- Purification of the Enzyme and Synthesis of a Fluorogenic Substrate

Walter F. Mangel and William J. McGrath

20. Cofactors of the Adenovirus Proteinase: Measuring Equilibrium Dissociation Constants and Stoichiometries of Binding

Walter F. Mangel and William J. McGrath

21. Characterization of the Adenovirus Fiber Protein

Jeffrey A. Engler and Jeong Shin Hong

22. Phylogenetic Analysis of Adenovirus Sequences

Balázs Harrach and Mária Benkö

23. Assessment of Genetic Variability Among Subspecies B1 Human Adenoviruses for Molecular Epidemiology Studies

Adriana E. Kajon and Dean D. Erdman

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