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Doody's Review ServiceReviewer: Rebecca T. Horvat, PhD, D (ABMM)(University of Kansas Medical Center)
Description: This is part of a two-volume series focusing on various methods used to examine the interaction of adenoviruses with host cells. Each chapter provides experimental details of various techniques used to evaluate adenovirus infections.
Purpose: The main goal of this book is to give detailed instructions on the laboratory procedures used to study adenovirus infections. According to the editors, this book focuses on methods used to evaluate and understand the interaction of these viruses with the host.
Audience: This book is intended for experienced individuals currently working in cell culture laboratories. It gives a great deal of detail on each technique. This would be useful for laboratory researchers at the bench but would not be helpful to students just learning this area. All of the authors are experts in their area and have had much experience with these techniques.
Features: Organized like its companion, this volume focuses specifically on measuring transcription, RNA splicing, and in vitro transcription. It also gives the details on techniques that can be used to study protein functions such as RNA interference, fluorescent labeling, co-immunoprecipitation and co-localization of proteins within the infected cell. Many of these techniques could be used to study the cellular interaction of other viruses or intracellular pathogens. Many chapters have good illustrations that show the result of these experimental techniques while other chapters have no illustrations. As in the first volume, the notes at the back of the chapter make them hard to use. It would be preferable to include the notes in the procedure.
Assessment: This is a very detailed techniques book. I would recommend it to investigators working in the area of viral-host interactions. Many of the chapters give very good detailed procedures on techniques used to study this dynamic interaction between a host and pathogen.