Adenovirus Methods and Protocols: Volume 2: Ad Proteins and RNA, Lifecycle and Host Interactions, and Phyologenetics / Edition 2

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Adenovirus Methods and Protocols, Second Edition, now in two volumes, is an essential resource for adenovirus (Ad) researchers beginning in the field, and an inspirational starting point for researchers looking to branch into new areas of Ad study. In addition to updating and expanding important chapters from the first edition, the authors have added new chapters that address innovative, exciting areas of emphasis in Ad research, including Ad vector construction and use, real-time PCR, use of new animal models, and methods for quantification of Ad virus or virus expression/interactions. The protocols presented are written by trendsetting researchers.

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Editorial Reviews

Doody's Review Service
Reviewer: Rebecca T. Horvat, PhD, D (ABMM)(University of Kansas Medical Center)
Description: This is part of a two-volume series focusing on various methods used to examine the interaction of adenoviruses with host cells. Each chapter provides experimental details of various techniques used to evaluate adenovirus infections.
Purpose: The main goal of this book is to give detailed instructions on the laboratory procedures used to study adenovirus infections. According to the editors, this book focuses on methods used to evaluate and understand the interaction of these viruses with the host.
Audience: This book is intended for experienced individuals currently working in cell culture laboratories. It gives a great deal of detail on each technique. This would be useful for laboratory researchers at the bench but would not be helpful to students just learning this area. All of the authors are experts in their area and have had much experience with these techniques.
Features: Organized like its companion, this volume focuses specifically on measuring transcription, RNA splicing, and in vitro transcription. It also gives the details on techniques that can be used to study protein functions such as RNA interference, fluorescent labeling, co-immunoprecipitation and co-localization of proteins within the infected cell. Many of these techniques could be used to study the cellular interaction of other viruses or intracellular pathogens. Many chapters have good illustrations that show the result of these experimental techniques while other chapters have no illustrations. As in the first volume, the notes at the back of the chapter make them hard to use. It would be preferable to include the notes in the procedure.
Assessment: This is a very detailed techniques book. I would recommend it to investigators working in the area of viral-host interactions. Many of the chapters give very good detailed procedures on techniques used to study this dynamic interaction between a host and pathogen.
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Product Details

  • ISBN-13: 9781617378355
  • Publisher: Springer-Verlag New York, LLC
  • Publication date: 11/10/2010
  • Series: Methods in Molecular Medicine Series, #131
  • Edition description: Softcover reprint of hardcover 2nd ed. 2007
  • Edition number: 2
  • Pages: 362
  • Product dimensions: 9.00 (w) x 6.00 (h) x 0.84 (d)

Table of Contents

Ann E. Tollefson, Mohan Kuppuswamy, and William S. M. Wold


Vol. II Topics: Ad proteins and RNA, lifecycle and host interactions, and phylogenetics

1. Analysis of the Efficiency of Adenovirus Transcription

Cristina Iftode and S. J. Flint

2. The Use of In Vitro Transcription to Probe Regulatory Functions of Viral Protein Domains

Paul M. Loewenstein, Chao-Zhong Song and Maurice Green

3. Preparation of Soluble Extracts from Adenovirus Infected Cells for Studies of RNA Splicing

Oliver Mühlemann and Göran Akusjärvi

4. In Vitro Methods to Study RNA Interference During an Adenovirus Infection

Gunnar Andersson, Ning Xu and Göran Akusjärvi

5. Simultaneous Detection of Adenovirus RNA and Cellular Proteins by Fluorescent Labeling In Situ

Eileen Bridge

6. Study of Nucleolar Localization of Adenovirus Core Proteins

David A. Matthews

7. Analysis of Adenovirus Infections in Synchronized Cells

David A. Ornelles, Robin N. Shepard and Felicia D. Goodrum

8. Co-Immunoprecipitation of Protein Complexes

Peter Yaciuk

9. Chromatin Immunoprecipitation (ChIP) to Study the Binding of Proteins to the Adenovirus Genome in Vivo

Jihong Yang and Patrick Hearing

10. Assaying Protein-DNA Interactions In Vivo and In Vitro Using Chromatin Immunoprecipitation and Electrophoretic Mobility Shift Assays

Pilar Perez-Romero and Michael J. Imperiale

11. Identifying Functional Adenovirus-Host Interactions Using Tandem Mass Spectrometry

Anuj Gaggar, Dmitry Shayakhmetov, and Andre Lieber

12. The Use of Cell Microinjection for the in vivo Analysis of Viral Transcriptional Regulatory Protein Domains

Maurice Green, Andrew Thorburn, Robert Kern and Paul M. Loewenstein

13. Determination of the Transforming Activities of Adenovirus Oncogenes

Michael Nevels and Thomas Dobner

14. Human Adenovirus Type 12: Crossing Species Barriers to Immortalize the Viral Genome

Walter Doerfler

15. Measurement of NK Cell Lytic Activity of Adenovirus-Infected or Adenovirus-Transformed Cells

John M. Routes

16. A Flow Cytometric Assay for Analysis of NK Cell-Mediated Cytolysis of Adenovirus-Transformed Cells

Graham Bottley, Graham P. Cook, and G. Eric Blair

17. Large Scale Purification and Crystallization of Adenovirus Hexon

John J. Rux and Roger M. Burnett

18. Synthesis and Assay of Recombinant Adenovirus Protease

Joseph M. Weber

19. Assay for the Adenovirus Proteinase- Purification of the Enzyme and Synthesis of a Fluorogenic Substrate

Walter F. Mangel and William J. McGrath

20. Cofactors of the Adenovirus Proteinase: Measuring Equilibrium Dissociation Constants and Stoichiometries of Binding

Walter F. Mangel and William J. McGrath

21. Characterization of the Adenovirus Fiber Protein

Jeffrey A. Engler and Jeong Shin Hong

22. Phylogenetic Analysis of Adenovirus Sequences

Balázs Harrach and Mária Benkö

23. Assessment of Genetic Variability Among Subspecies B1 Human Adenoviruses for Molecular Epidemiology Studies

Adriana E. Kajon and Dean D. Erdman

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