Adenovirus Methods and Protocols: Volume 1: Adenoviruses, Ad Vectors, Quantitation, and Animal Models / Edition 2

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Adenovirus Methods and Prools, Second Edition, now in two volumes, is an essential resource for adenovirus (Ad) researchers beginning in the field, and an inspirational starting point for researchers looking to branch into new areas of Ad study. In addition to updating and expanding the first edition, the authors have added new chapters that address innovative areas of emphasis in Ad research, including Ad vector construction and use, real-time PCR, use of new animal models, and methods for quantification of Ad virus or virus expression/interactions. Each of the prools presented in these volumes is written by trendsetting researchers.

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Editorial Reviews

Doody's Review Service
Reviewer: Rebecca T. Horvat, PhD, D (ABMM)(University of Kansas Medical Center)
Description: As the title implies, this book provides details on the procedures that can be used to construct adenoviruses to be used as vectors. Each chapter specifically details laboratory procedures that can be used for these purposes.
Purpose: The book is intended to provide a "detailed description" of the methods used to grow, detect, and analyze adenoviruses. The overall purpose of growing and detecting these viruses is so that they can be used as vectors to transfer genes into cells or larger animals. This is of great interest now, since this could potentially provide a means of delivering genetic material as a drug.
Audience: Scientists working in the area of virology and gene transfer are the intended audience, but the book would be easy reading for most levels from the undergraduate student to the professional scientist. All the authors clearly have personal experience in using these techniques.
Features: The 18 chapters cover primarily the procedures used to grow adenoviruses and genetically manipulate the virus as well as the use of animal models to test the manipulated viruses. Each chapter starts with a summary and an introductory section, followed by a section on materials needed to perform the experimental protocols and then by specific methods to accomplish the experiments using adenoviruses. These sections are followed by notes on the procedures and are linked to the procedure by a number. Overall there is a great deal of information on laboratory technique. Some authors list very similar techniques, which can be confusing for readers trying to make decisions on protocols. It was difficult to continually go back to the notes section and then find where you left the procedure. It would help to place the notes close to where the reference is in the protocol. Also, some of the techniques seem dated and might be hard to perform in a laboratory just starting in this area. Overall, though, the book provides some good advice on using and growing adenovirus vectors.
Assessment: This book would be useful for scientists in this area. A change in the size and format of the book would help. It does not stay open very well and at a lab bench, this could be annoying. It would also be useful to have the hints and notes embedded into the methods, making it clearer for the user.
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Product Details

  • ISBN-13: 9781617376696
  • Publisher: Springer-Verlag New York, LLC
  • Publication date: 11/10/2010
  • Series: Methods in Molecular Medicine Series, #130
  • Edition description: Softcover reprint of hardcover 2nd ed. 2007
  • Edition number: 2
  • Pages: 242
  • Product dimensions: 9.00 (w) x 6.00 (h) x 0.58 (d)

Table of Contents

TABLE OF CONTENTS Vol. I Topics: Adenoviruses and vectors, quantitation, and animal models

1. Manipulation of Early Region 4
Julie Boyer and Gary Ketner
2. Isolation, Growth, and Purification of Defective Adenovirus Deletion Mutants Gary Ketner and Julie Boyer
3. Construction of Adenovirus Type 5 Early Region 1 (E1) and 4 (E4) Virus Mutants Peter Groitl and Thomas Dobner
4. Construction of Mouse Adenovirus Type 1 Mutants Angela N. Cauthen, Amanda R. Welton, and Katherine R. Spindler
5. Generation of Recombinant Adenovirus using E.coli BJ5183 Recombination System P. Seshidhar Reddy, Shanthi Ganesh, Lynda Hawkins, Neeraja Idamakanti
6. Production and Release Testing of Ovine AtadenovirusVectors Gerald W. Both, Fiona Cameron, Anne Collins, Linda J. Lockett, Jan Shaw
7. Construction of Capsid Modified Recombinant Bovine Adenovirus Type 3
Alexander N. Zakhartchouk, Qiaohua Wu, Suresh K. Tikoo
8. Adenovirus Capsid Chimeras: Fiber Terminal Exon Insertions / Gene Replacements in the MLTU Jason Gall, John Schoggins, and Erik Falck-Pedersen
9. Temperature-Sensitive Replication-Competent Adenovirus shRNA Vectors to Study Cellular Genes in Virus-Induced Apoptosis T. Subramanian and G. Chinnadurai

10. Evaluating Apoptosis in Tumor Cells Infected with Adenovirus Expressing p53 Tumor Suppressor Gene Elizabeth Perron, M. Behzad Zafar, Amanda Pister, Zhen-Guo Wang, Jun Tian and Prem Seth
11. Growth and Purification of Enteric Adenovirus Type 40
Vivien Mautner
12. Immunocompetent, Semi-permissive Cotton Rat Tumor Model for the Evaluation of Oncolytic Adenoviruses Karoly Toth, Jacqueline F. Spencer, William S.M. Wold
13. The Use of the Syrian Hamster as an Animal Model for Oncolytic Adenovirus Vectors Maria A. Thomas, Jacqueline F. Spencer, and William S. M. Wold
14. A Real-Time PCR Method to Rapidly Titer Adenovirus Sks Maria A. Thomas, Drew L. Lichtenstein, Peter Krajcsi, and William S. M. Wold
15. Detection and Quantitation of Subgroup C Adenovirus DNA in Human Tissue Samples by Real-Time PCR C.T. Garnett, Ching-I Pao, Linda R. Gooding
16. Flow Cytometric Detection of Adenoviruses and Intracellular Adenovirus Proteins Graham Bottley, John R. Holt, Nicola J. James and G. Eric Blair
17. Capture ELISA Quantitation of Mouse Adenovirus Type 1 in Infected Organs Amanda R. Welton and Katherine R. Spindler
18. Preparation and Titration of CsCl-Banded Adenovirus Sks

Ann E. Tollefson, Mohan Kuppuswamy, Elena V. Shashkova, Konstantin Doronin, and William S. M. Wold

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