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More About This Textbook
Overview
Prools books specializing in measuring free radical and antioxidant biomarkers began to be published in 1998. Many of these methods are currently finding use in diagnostic medicine. Advanced Prools in Oxidative Stress I covers the field of oxidative stress with state-of-the-art technology to utilize in research, contributed by an international panel of experts renowned for developing new procedures and methods. Included are sections on reactive oxygen and nitrogen species techniques, antioxidant technology and application, methods for analyzing gene expression, the exciting new area of oxidative stress and stem cell differentiation and specific biostatistical evaluation of biomarkers. This volume presents the current high-tech methodologies and provides a perspective on the diversity of applications in the ever-emerging field of free radical reactions and antioxidants. Due to the dynamic nature of this topic, this book will be the first of several volumes of Advanced Prools in Oxidative Stress, all part of the highly successful Methods in Molecular Biology™ series. As part of the series, the chapters include a brief introduction to the material, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory prools, and tips on troubleshooting and ensuring replication of technology.
Cutting-edge and convenient, Advanced Prools in Oxidative Stress I is an ideal desk reference for scientists wishing to further this research in this exciting, unique and vital field of study.
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Table of Contents
Part I. Reactive Oxygen and Nitrogen Techniques
1. Novel Designed Probes for the Characterization of Oxidative Stress in Biological Fluids, Cell and Tissues
Jacob Vaya
2. A Rapid and Selective Mass Spectroscopic Method for the Identification of Nitrated Proteins
Angela Amoresano, Giovanni Chiappetta, Piero Pucci and Gennaro Marino
3. An Easy and Reliable Automated Method to Estimate Oxidative Stress in the Clinical Setting
Cristina Vassalle
4. Correlative Transmission Microscopy: Cyhemical Localization and Immunocyhemical Localization in Studies of Oxidative and Nitrosative Stress
E. Ann Ellis
5. Detection of Specifically Oxidized Apolipoproteins in Oxidized HDL
Xiao Suo Wang and Roland Sker
6. High Performance Liquid Chromatography / Electron Spin Resonance / Mass Spectroscopy Analysis of Lipid-Derived Radicals
Hideo Iwahashi
7. EPR Spin-Trapping and Nano LC MS/MS Techniques for DEPMPO/ OOH and Immunospin-trapping with Anti-DMPO Antibody in Mihondrial Electron Transfer System
Yeong-Renn Chen
8 Determination of High Mihondrial Membrane Potential in Spermatazoa loaded with the Mihondrial Probe 5, 5’, 6, 6’- tetrachloro-1, 1’, 3, 3’-tetraethylbenzimazoyolyl-carbocyanine iodide ( JC-! ) and for Using Fluorescence-activated Flow Cytometry
H. David Guthrie and Glenn R. Welch
9. TEMPO Probes for Evaluating Oxidative Stress on the Cell Membrane and Mihondria
Hidehiko Nakagawa and Naoki Miyata
10. Zymographical Techniques for Detection of Matrix Metallproteinases
Shinichi Iwai, Takako Nakanishi-Ueda , Donald Armstrong and Katsuji Oguchi
11. The Use of in vivo Microanalysis Techniques to Detect Exracellular ROS in Resting and Contracting Skeletal Muscle
Graeme L. Close and Malcolm J. Jackson
12. Cell-free Antibody Capture Method for Analysis of Detergent-Resistant Membrane Rafts
Anil Bamezai and Colleen Kennedy
13. Determination of Acrolein by High Voltage Capillary Electrophoresis from Oxidized Fatty Acids
Rafael Medina-Navarro
Part II. Antioxidant Technology and Application
14. CUPRAC ( cupric ion reducing antioxidant capacity ) Assay for Food Antioxidants, Vitamins, Polyphenols and Flavonoids in Food Extracts
Resat Apak , Kubilay Güçlü, Mustafa Özyürek, Burcu Bektasoglu and Mustafa Bener
15. Redox Property of Ribonucleotide Reductase Small Subunit M2 and p53R2
Xiyong Liu, Lijun Xue and Yun Yen
16. Antioxidant QSAR Modeling as Exemplified on Polyphenols
Bono Lucic, Dragan Amic and Nenad Trinajstic
17. Immunohishemical Staining of Cyclooxygenases with Monoclonal Antibodies
Ghassan M. Saed
18. Examining the Endogenous Antioxidant Response Through Immunofluorescent Analysis of Nrf2 in Tissue
Kathryn A. Lindl and Kelly L. Jordan-Sciutto
19. Determination of Oxidized and Reduced CoQ10 and CoQ9 in Human Plasma / Serum Using HPLC-ECD
Ian N. Acworth, Paul A. Ullucci and Paul H. Gamache
20. Paraoxonases ( PON1 , PON2 , PON3 ) Analysis in vitro and in vivo in Relation to Cardiovascular Diseases
Michael Aviram and Mira Rosenblat
21. Preparation, Characterization and Use of Antioxidant-Liposomes
Hongsong Yang, Victor Paromov, Milton Smith and William L. Stone
22. Antioxidant Activity of Biotransformed Sex Hormones Facilitated by Bacillus Stearothermophilus
Mohammad Afzal, Sameera Al-Awadi and Sosamma Oommen
23. Biolistic Mediated Production of Transgenic Oil Palm
Ghulam Kadir Ahmed Parveez
24. Preparation of a Multi-Antioxidant Formulation
John A. Mulnix and Brook E. Stoddard
Part III. Gene Expression
25. A Functional Genomics Approach to Identify and Characterize Oxidation Resistance Genes
Michael R. Volkert, Jen-Yeu Wang and Nathan A. Elliott
26. Genome-Wide Overexpression Screen for Activators of Antioxidant Gene Transcription
Hendrik Luesch and Yanxia Liu
27. Method for Conducting Microarray Study of Oxidative Stress Induced Gene Expression
Sita Subbaram, Juan A. Melendez and Sridar V. Chittur
28. A Systems Approach Demonstrating Sphingolipid-Dependent Transcription in Stress Responses
Alan J. Wilder and L. Ashley Cowart
29. Bioluminescence : Imaging Modality for in vitro and in vivo Gene Expression
Ruxana T. Sadikot and Timothy S. Blackwell
Part IV. Stem Cells
30. Reactive Oxygen Species and Upregulation of NADPH Oxidases in Mechanotransduction of Embryonic Stem Cells
Heinrich Sauer, Carola Ruhe, Jörg P. Müller, Maike Schmelter, Rochelle D’Souza and Maria Wartenberg
Part V. Biostatistics
31. Pooling Data When Analyzing Biomarkers Subject to a Limit of Detection
Leslie Rosenthal and Enrique Schisterman