Affinity Chromatography: Methods and Protocols

by Michael Zachariou (Editor)

Overview

Thirty-eight years after its introduction, affinity chromatography remains a key tool in the armory of separation techniques available to separation and interaction scientists. Expanded and updated from the first edition, this second edition volume aims to provide the beginner with the practical knowledge needed to develop affinity separations suitable for a variety of applications relevant to the post-genomic era. It contains state-of-the-art, brand new protocols, and provides step-by-step laboratory instructions for readily reproducible results.

Product Details

ISBN-13:	9781617377013
Publisher:	Springer-Verlag New York, LLC
Publication date:	11/19/2010
Series:	Methods in Molecular Biology , #421
Edition description:	Softcover reprint of hardcover 2nd ed. 2008
Pages:	348
Product dimensions:	5.98(w) x 9.02(h) x 0.35(d)

Table of Contents

Preface.- Contributors.- Affinity Chromatography: History, Perspectives, Limitations and ProspectsAna Ceclia A. Roque and Christopher R. Lowe 1.- Various Modes of Affinity Chromatography.- Immobilized Metal Ion Affinity Chromatography of Native ProteinsAdam Charlton and Michael Zachariou.- Affinity Precipitation of Proteins Using Metal ChelatesAshok Kumar, Igor Yu. Galaev, and Bo Mattiasson.- Immunoaffinity ChromatographyStuart R. Gallant, Vish Koppaka, and Nick Zecherle.- Dye Ligand ChromatographyStuart R. Gallant, Vish Koppaka, and Nick Zecherle.- Purification of Proteins Using Displacement ChromatographyNihal Tugcu.- Affinity Chromatography Using Purification Tags.- Rationally Designed Ligands for Use in Affinity Chromatography: An Artificial Protein LAna Ceclia A. Roque and Christopher R. Lowe.- Phage Display of Peptides in Ligand Selection for Use in Affinity ChromatographyJoanne L. Casey, Andrew M. Coley, and Michael Foley.- Preparation, Analysis and Use of an Affinity Adsorbent for the Purification of GST Fusion ProteinGareth M. Forde.- Immobilized Metal Ion Affinity Chromatography of Histidine-Tagged Fusion ProteinsAdam Charlton and Michael Zachariou.- Methods for the Purification of HQ-Tagged ProteinsBecky Godat, Laurie Engel, Natalie A. Betz, and Tonny M. Johnson.- Amylose Affinity Chromatography of Maltose-Binding Protein: Purification by both Native and Novel Matrix-Assisted Dialysis Refolding MethodsLeonard K. Pattenden and Walter G. Thomas.- Methods for Detection of Protein–Protein and Protein–DNA Interactions Using HaloTagMarjeta Urh, Danette Hartzell, Jacqui Mendez, Dieter H. Klaubert, and Keith Wood.- Site-Specific Cleavage of Fusion ProteinsAdam Charlton.- The Use of TAGZyme for the Efficient Removal of N-Terminal His-TagsJose? Arnau, ConniLauritzen, Gitte Ebert Petersen, and John Pedersen.- Various Applications of Affinity Chromatography.- Affinity Processing of Cell-Containing Feeds Using Monolithic Macroporous Hydrogels, CryogelsIgor Yu. Galaev and Bo Mattiasson.- Monolithic Bioreactors for MacromoleculesMojca Ben?ina, Katja Ben?ina, Aleš Podgornik, and Aleš Štrancar.- Plasmid DNA Purification Via the Use of a Dual Affinity ProteinGareth M. Forde.- Affinity Chromatography of Phosphorylated ProteinsGrigoriy S. Tchaga.- Protein Separation Using Immobilized Phospholipid ChromatographyTzong-Hsien Lee and Marie-Isabel Aguilar.- Analysis of Proteins in Solution Using Affinity Capillary ElectrophoresisNiels H. H. Heegaard, Christian Schou, and Jesper Østergaard.

Editorial Reviews

From the reviews of the second edition:

"The book consists of three parts including 21 chapters that have been written by a large group of authors from many countries. … recommends this book as a manual for many specialists involved in purification of biomolecules in various fields of biochemistry, molecular biology, biotechnology, and bioorganic chemistry. The book can also be recommended as a handbook for university students and their teachers as well as for other schools preparing specialists in the above-mentioned fields." (G. Ya. Wiederschain, Biochemistry, November, 2008)

From the Publisher