Agrobacterium Protocols: Volume I / Edition 2

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A gold standard collection of Agrobacterium-mediated transformation techniques for state-of-the-art plant genetic engineering, functional genomic analysis, and crop improvement. Volume 1 details the most updated techniques available for twenty-six plant species drawn from cereal crops, industrial plants, legume plants, and vegetable plants, and presents various methods for introducing DNA into three major model plant species, Arabidopsis thaliana, Medicago truncatula, and Nicotiana. The authors also outline the basic methods in Agrobacterium manipulation and strategies for vector construction. Volume 2 contains another thirty-three proven techniques for root plants, turf grasses, woody species, tropic plants, nuts and fruits, ornamental plants, and medicinal plants. Additional chapters provide methods for introducing DNA into non-plant species, such as bacteria, fungi, algae, and mammalian cells. The prools follow the successful Methods in Molecular Biology™ series format, each offering step-by-step laboratory instructions, an introduction outlining the principles behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls.

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Editorial Reviews

From the Publisher
From the reviews of the second edition:

"This book is intended as a laboratory manual for researchers using Agrobacterium tumefaciens for plant transformation. It covers the basics of culturing and transforming Agrobacterium … . You don’t expect a lab manual to be ideal … reading, but I found this to be surprisingly readable. … The book would be especially useful for someone just starting plant transformation work or changing to new plant species and … it would also be useful reading for Masters students." (Heather Macdonald, Microbiology Today, November, 2006)

"Methods in Molecular Biology opens … a field concerning Agrobacterium-mediated transformation of plants. … Each chapter is written by the leader in the field … and offers a detailed manual of the transformation prool. … All users of this book will certainly appreciate the notes sections that bring additional information on potential difficulties in the prools and alternative materials or methods. The book is the fountainhead of recent practical knowledge concerning Agrobacterium-mediated transformation and … it will become the core manual … ." (J. BRlZA, Biologia Plantarum, Vol. 51 (2), 2007)

"The book’s chapters are organized in a highly detailed fashion, providing readers not only with step-by-step prools … but also with a comprehensive list of equipment and materials. … With such a rich source of information, organized to be easy to read and very simple to implement, the book should be considered a must for every plant biology laboratory. … It will also be an excellent tool for training students, doctorates, technicians, and other laboratory personnel in the art of plant genetic transformation." (Tzvi Tzfira, Quarterly Review of Biology, Vol. 82, March, 2007)

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Product Details

  • ISBN-13: 9781588295361
  • Publisher: Springer-Verlag New York, LLC
  • Publication date: 6/28/2006
  • Series: Methods in Molecular Biology Series , #343
  • Edition description: 2nd ed. 2006
  • Edition number: 2
  • Pages: 484
  • Product dimensions: 6.20 (w) x 9.30 (h) x 1.30 (d)

Table of Contents

Part I. Agrobacterium Handling

Culture and Maintenance of Agrobacterium Strains
Arlene A. Wise, Zhenying Liu, and Andrew N. Binns

Binary Vectors and Super-binary Vectors
Toshihiko Komari, Yoshimitsu Takakura, Jun Ueki, Norio Kato, Yuji Ishida, and Yukoh Hiei

Three Methods for the Introduction of Foreign DNA into Agrobacterium
Arlene A. Wise, Zhenying Liu, and Andrew N. Binns

Integration of Genes into the Chromosome of Agrobacterium tumefaciens C58
Lan-Ying Lee

Nucleic Acid Extraction from Agrobacterium Strains
Arlene A. Wise, Zhenying Liu, and Andrew N. Binns

Agrobacterium Virulence Gene Induction
Stanton B. Gelvin

Part II. Model Plants

Arabidopsis thaliana Floral Dip Transformation Method
Andrew Bent

Agrobacterium Transformation of Arabidopsis thaliana Roots: A Quantitative Assay
Stanton B. Gelvin

Medicago truncatula Transformation Using Leaf Explants
Viviane Cosson, Patricia Durand, Isabelle d'Erfurth, Adam Kondorosi, and Pascal Ratet

Medicago truncatula Transformation Using Cotyledon Explants
Elane Wright, Richard A. Dixon, and Zeng-Yu Wang

Medicago truncatula Transformation Using Root Explants
Cynthia Crane, Richard A. Dixon, and Zeng-Yu Wang

Nicotiana (Nicotiana tobaccum, Nicotiana benthamiana)
Tom Clemente

Generation of Composite Plants Using Agrobacterium rhizogenes
Christopher G. Taylor, Beth Fuchs, Ray Collier, and W. Kevin Lutke

Part III. Cereal Crops

Barley (Hordeum vulgare L.)
John Jacobsen, Ingrid Venables, Ming-Bo Wang, Peter Matthews, Michael Ayliffe, and Frank Gubler

Maize (Zea mays L.)
Bronwyn R. Frame, Tina Paque, and Kan Wang

Indica Rice (Oryza sativa, BR29 and IR64)
Karabi Datta and Swapan Kumar Datta

Japonica Rice Varieties (Oryza sativa, Nipponbare, and Others)
Philippe Hervé and Toshiaki Kayano

Rye (Secale cereale L.)
Fredy Altpeter

Sorghum (Sorghum bicolor L.)
Zuo-yu Zhao

Wheat (Triticum aestivum L.)
Yuechun Wan and Jeanne Layton

Part IV. Industrial Plants

Canola (Brassica napus L.)
Vinitha Cardoza and C. Neal Stewart, Jr.

Cotton (Gossypium hirsutum L.)
Keerti S. Rathore, Ganesan Sunilkumar, and LeAnne M. Campbell

Indian Mustard [Brassica juncea (L.) Czern.]
Ksenija Gasic and Schuyler S. Korban

Sunflower (Helianthus annuus L.)
Dalia M. Lewi, H. Esteban Hopp, and Alejandro S. Escandón

Part V. Legume Plants

Alfalfa (Medicago sativa L.)
Deborah A. Samac and Sandra Austin-Phillips

Chickpea (Cicer arietinum L.)
Kiran Kumar Sharma, Pooja Bhatnagar-Mathur, and Boddu Jayanand

Clovers (Trifolium spp.)
Aidyn Mouradov, Stephen Panter, Marcel Labandera, Emma Ludlow, Michael Emmerling, and German Spangenberg

Peas (Pisum sativum L.)
Jan Grant and Pauline Cooper

Peanut (Arachis hypogaea L.)
Kiran Kumar Sharma and Pooja Bhatnagar-Mathur

Pigeonpea (Cajanus cajan L. Millsp.)
Kiran Kumar Sharma, Gopinath Sreelatha, and Sunitha Dayal

Red Clover (Trifolium pratense)
Michael L. Sullivan and Kenneth H. Quesenberry

Soybean (Glycine max) Transformation Using Mature Cotyledonary Node Explants
Paula M. Olhoft, Christopher M. Donovan, and David A. Somers

Soybean (Glycine max) Transformation Using Immature Cotyledon Explants
Tae-Seok Ko, Schuyler S. Korban, and David A. Somers

Tepary Bean (Phaseolus acutifolius)
Mukund Zambre, Marc Van Montagu, Geert Angenon, and Nancy Terryn

Part VI. Vegetable Plants

Brassica oleracea
Penny A. C. Sparrow, Philip J. Dale, and Judith A. Irwin

Cucumber (Cucumis sativus

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