Alcamo's Laboratory Fundamentals Of Microbiology / Edition 10

Other Format (Print)
Rent from
(Save 67%)
Est. Return Date: 07/04/2015
Buy Used
Buy Used from
(Save 33%)
Item is in good condition but packaging may have signs of shelf wear/aging or torn packaging.
Condition: Used – Good details
Used and New from Other Sellers
Used and New from Other Sellers
from $49.75
Usually ships in 1-2 business days
(Save 49%)
Other sellers (Other Format)
  • All (12) from $49.75   
  • New (5) from $61.15   
  • Used (7) from $49.75   

More About This Textbook


Updated with new exercises and a new organization, the eighth edition of Alcamo's Laboratory Fundamentals of Microbiology is a comprehensive lab manual offering thirty-four multipart lab exercises designed to provide students with basic training in the handling of microorganisms while exploring microbial properties and uses. It is reorganized to introduce basic laboratory skills-culture transfer and pure culture techniques-in the first two exercises, followed by microscopy and bacterial staining techniques. Included in this edition are new exercises for analysis of a bacterial growth curve, bacterial conjugation, and bacterial transformation. This lab manual can be used independently of Alcamo's Fundamentals of Microbiology, Eigth Edition. An Instructor's Manual is also available and provides principles of lab safety; research topic ideas; information on customizing laboratory programs; helpful suggestions for setting up and running each exercise; and lists of laboratory media, cultures, and special materials used in each exercise.
Read More Show Less

Product Details

  • ISBN-13: 9781284031072
  • Publisher: Jones & Bartlett Learning
  • Publication date: 2/8/2013
  • Format: Spiral Bound
  • Edition description: New Edition
  • Edition number: 10
  • Pages: 356
  • Sales rank: 611,846
  • Product dimensions: 8.30 (w) x 10.80 (h) x 0.80 (d)

Table of Contents

Preface     vii
An Important Message About Safety     ix
Basic Laboratory Techniques and Skills     1
Culture Transfer Techniques     5
Aseptic Transfer Techniques     5
Pure Culture Techniques     11
Streak Plate Technique     11
Pour Plate Technique     14
Microscopy and Bacterial Staining Techniques     21
The Compound Microscope     23
Parts of the Microscope and Their Use     23
Observations of Prepared Slides     25
Preparation of a Bacterial Smear and the Simple Stain Technique     35
Preparation of a Bacterial Smear     35
The Simple Stain Technique     38
The Negative Stain Technique     43
The Gram Stain Technique     49
Bacterial Structures     55
Spore Stain Technique     55
Capsule Stain Technique     57
Bacterial Motility     59
Viruses and Eukaryotic Microorganisms     65
Viruses     67
Constructing a Virus     67
The Effect of Bacteriophages on Bacteria     68
Plaque Formation     70
Identification of Bacteriophages from Sewage     72
Estimating the Number of Bacteriophages     72
Fungi, Protozoa, and Multicellular Parasites     79
Fungi (Molds and Yeasts)     79
Protozoa and Multicellular Parasites     84
The Spread and Control of Microorganisms     91
A Simulated Epidemic     93
Microbial Transmission via Fomites     93
A Microbial Hunt     95
Transmission of Microorganisms through Toilet Paper     97
The Effect of Physical Agents on Bacteria     101
Heat Tolerance     101
Ultraviolet Light     103
Drying     105
Other Physical Agents     106
The Effect of Chemical Agents on Bacteria     111
Antiseptics and Disinfectants     111
Metals     113
Other Chemical Substances     114
Effect of Lysozyme on Bacteria     115
Disinfection of Drinking Water     116
Evaluation of Disinfectants and Antiseptics     125
Effectiveness of Disinfectants on Inanimate Objects     125
Effectiveness of Disinfectants and Hand Washing on the Skin Surface     126
Mouthwashes and Oral Bacteria     127
The Phenol Coefficient     128
The Effect of Antibiotics on Bacteria     135
Microbial Growth: Analysis of a Bacterial Growth Curve     145
Bacterial Growth Dynamics     146
Plotting a Growth Curve and Determining the Generation Time     147
Medical Microbiology     151
The Genus Mycobacterium     153
Colony and Cellular Morphology     153
Acid-Fast Stain Technique     154
Cold Acid-Fast Stain Technique     156
The Genus Streptococcus     159
Streptococci from the Upper Respiratory Tract     159
Streptococci from the Oral Cavity     161
The Genus Neisseria     167
The Genus Staphylococci     171
Isolation of Staphylococcus     171
Differentiation between Staphylococci Species     173
The Enteric Bacteria     179
Isolation of Enteric Bacteria     179
Differentiation of Enteric Bacteria on TSI Agar     180
The IMViC Series     182
Rapid Identification of Enteric Bacteria     183
The Genus Bacillus     191
The Genus Clostridium     195
The Genus Lactobacillus     201
Isolation of Lactobacilli     201
Caries Susceptibility Test      202
Identification of a Bacterial Unknown     207
Bacterial Structural Characteristics     213
Bacterial Culture Characteristics     217
Biochemical Characteristics of Bacteria     223
Carbohydrate Fermentation     223
Starch Digestion     224
Catalase Production     226
DNA Digestion     227
Hydrogen Sulfide Production     228
IMViC Series     229
Urea Digestion     230
Lipid Digestion     230
Casein Digestion     231
Bacterial Genetics and Serology     239
Bacterial Genetics: Mutations and the Ames Test     241
Gradient Plate Technique     242
Mutagenic Effect of Ultraviolet Light     243
DNA Repair of UV Light Damage     245
The Ames Test     246
Bacterial Conjugation     253
Bacterial Transformation     259
Serology: Agglutination Techniques and Blood Cell Identification     267
Slide Agglutination     267
Tube Agglutination     268
Hemagglutination: Determination of Blood Type     270
Blood Smear     272
Public Health and Environmental Microbiology      277
Microbiology of Foods     279
Food Preservation with Salt and Garlic     279
Standard Plate Count of Food Products     280
Fermentation of Sauerkraut     283
Fermentation of Wine and Beer     284
Microbiology of Milk and Dairy Products     293
Standard Plate Count of Milk     293
Coliform Plate Count of Milk     295
Methylene Blue Reduction Test     296
Preparation of Cheese     297
Preparation of Yogurt     298
Natural Bacterial Content of Milk     299
Microbiology of Water     307
Presumptive Test by the MPN Method     307
Confirmed Test     308
Completed Test     310
Membrane Filter Technique     311
Preparation of a Biofilm     312
Microbiology of Soil     319
Isolation of Rhizobium from Legume Roots     319
Ammonification by Soil Microorganisms     320
Isolation of Streptomyces from Soil     321
Antibiotic Production by Streptomyces     321
Plate Count of Soil Bacteria     323
Microbial Ecology in the Soil-The Winogradsky Column     323
Recycling of Carbon in Food      325
Recycling of Carbon in Cloth     327
Recycling of Carbon in Leaves     328
Preparation of Stains and Diagnostic Reagents     337
Using Enterotube II     339
Index     343
Read More Show Less

Customer Reviews

Be the first to write a review
( 0 )
Rating Distribution

5 Star


4 Star


3 Star


2 Star


1 Star


Your Rating:

Your Name: Create a Pen Name or

Barnes & Review Rules

Our reader reviews allow you to share your comments on titles you liked, or didn't, with others. By submitting an online review, you are representing to Barnes & that all information contained in your review is original and accurate in all respects, and that the submission of such content by you and the posting of such content by Barnes & does not and will not violate the rights of any third party. Please follow the rules below to help ensure that your review can be posted.

Reviews by Our Customers Under the Age of 13

We highly value and respect everyone's opinion concerning the titles we offer. However, we cannot allow persons under the age of 13 to have accounts at or to post customer reviews. Please see our Terms of Use for more details.

What to exclude from your review:

Please do not write about reviews, commentary, or information posted on the product page. If you see any errors in the information on the product page, please send us an email.

Reviews should not contain any of the following:

  • - HTML tags, profanity, obscenities, vulgarities, or comments that defame anyone
  • - Time-sensitive information such as tour dates, signings, lectures, etc.
  • - Single-word reviews. Other people will read your review to discover why you liked or didn't like the title. Be descriptive.
  • - Comments focusing on the author or that may ruin the ending for others
  • - Phone numbers, addresses, URLs
  • - Pricing and availability information or alternative ordering information
  • - Advertisements or commercial solicitation


  • - By submitting a review, you grant to Barnes & and its sublicensees the royalty-free, perpetual, irrevocable right and license to use the review in accordance with the Barnes & Terms of Use.
  • - Barnes & reserves the right not to post any review -- particularly those that do not follow the terms and conditions of these Rules. Barnes & also reserves the right to remove any review at any time without notice.
  • - See Terms of Use for other conditions and disclaimers.
Search for Products You'd Like to Recommend

Recommend other products that relate to your review. Just search for them below and share!

Create a Pen Name

Your Pen Name is your unique identity on It will appear on the reviews you write and other website activities. Your Pen Name cannot be edited, changed or deleted once submitted.

Your Pen Name can be any combination of alphanumeric characters (plus - and _), and must be at least two characters long.

Continue Anonymously

    If you find inappropriate content, please report it to Barnes & Noble
    Why is this product inappropriate?
    Comments (optional)