Analytical Biochemistry

Overview

Now an established handbook of principles and techniques, this text develops an understanding of the relevance of four fundamental properties of the analyte: shape, polarity, charge and size, to the three key types of analysis: separation, identification and quantification. The third edition of Analytical Biochemistry has now been fully updated in content and format, making it even more accessible to students learning how to select analytical techniques and recognise their scope...

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1993 Trade paperback 2nd ed. NEW BOOK/slightly shelf worn & aged to cover-no international shipping avilable. Trade paperback (US). Glued binding. 507 p. Audience: General/trade.

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Overview

Now an established handbook of principles and techniques, this text develops an understanding of the relevance of four fundamental properties of the analyte: shape, polarity, charge and size, to the three key types of analysis: separation, identification and quantification. The third edition of Analytical Biochemistry has now been fully updated in content and format, making it even more accessible to students learning how to select analytical techniques and recognise their scope and limitations.

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Editorial Reviews

Booknews
Revised and updated coverage of the major biochemical analytical techniques, with explanation of underlying principles, discussion of scope and applications, and analysis of how to select appropriate techniques for particular situations. Topics are in three main sections on analytical techniques such as spectroscopy and chromatography; enzymes, antibodies, and radio-isotopes; and the chemical characteristics of the major groups of biologically important compounds and methods for their analysis. Annotation c. Book News, Inc., Portland, OR (booknews.com)
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Product Details

  • ISBN-13: 9780582066946
  • Publisher: Longman Publishing Group
  • Publication date: 1/1/1993
  • Edition description: 2nd ed
  • Edition number: 2
  • Pages: 528

Table of Contents

Preface to the second edition
Preface to the first edition
Abbreviations
1 General principles of analytical biochemistry 1
The selection of a valid method of analysis 1
The quality of data 5
The presentation of data 23
2 Spectroscopy 28
Interaction of radiation with matter 28
Molecular absorptiometry 40
Absorptiometer design 51
Molecular Fluorescence techniques 65
Atomic spectroscopy techniques 67
Magnetic resonance spectroscopy 77
3 Separation methods 83
Principles of separation techniques 84
Methods based on polarity 90
Methods based on ionic nature 121
Methods based on size 138
Methods based on shape 159
4 Electroanalytical methods 164
Potentiometry 165
Conductimetry 177
Coulometry 181
Voltammetry 185
Biosensors 189
5 Radio-isotopes 193
Nature of radioactivity 193
Detection and measurement of radioactivity 198
Biochemical uses of isotopes 202
6 Automated methods of analysis 206
Discrete analysers 208
Flow analysis 213
Robotics 230
7 Immunological methods 233
General processes of the immune response 233
Antigen - antibody reactions 241
Analytical techniques - precipitation reactions 244
Analytical techniques - immunoassay 249
8 Enzymes 261
The nature of enzymes 262
Monitoring techniques 277
Enzyme assay methods 296
Substrate assay methods 303
Immobilized enzymes 308
Treatment of cells and tissues for enzyme assay 312
9 Carbohydrates 318
General structure and function 318
Chemical methods of carbohydrate analysis 333
Enzymic methods of carbohydrate analysis 342
Separation and identification of carbohydrate mixtures 351
10 Amino acids 358
General structure and properties 359
General reactions 372
N-terminal analysis 376
Reactions of specific amino acids 379
Separation of amino acid mixtures 384
Amino acid analyser 391
11 Proteins 399
Protein structure 399
General methods of quantitation 404
Separation of proteins 413
12 Lipids 422
Fatty acids 423
Simple lipids 424
Complex lipids 431
Lipid - protein structures 438
Sample preparation and handling 440
Quantitative methods 442
Separation of lipid mixtures 446
13 Nucleic acids 461
Nucleic acid composition and structure 461
Isolation and purification of nucleic acids 467
Methods of nucleic acid analysis 473
Vectors 480
DNA sequencing 485
Appendix: Numbering and classification of enzymes 490
Index 497
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