Analytical Techniques

Analytical Techniques

by T.P. Mommsen, P.W. Ed. Hochachka
     
 

This third volume in the series covers such topics as anaesthetics, cannulation and injection techniques, and surgery. The book will be invaluable to fisheries scientists, aquaculturists, and animal biochemists, physiologists and endocrinologists; it will provide researchers and students with a pertinent information source from theoretical and experimental angles.See more details below

Overview

This third volume in the series covers such topics as anaesthetics, cannulation and injection techniques, and surgery. The book will be invaluable to fisheries scientists, aquaculturists, and animal biochemists, physiologists and endocrinologists; it will provide researchers and students with a pertinent information source from theoretical and experimental angles.

Product Details

ISBN-13:
9780444820327
Publisher:
Elsevier Science
Publication date:
10/25/1994
Series:
Biochemistry and Molecular Biology of Fishes Series
Pages:
720
Product dimensions:
1.63(w) x 9.21(h) x 6.14(d)

Related Subjects

Meet the Author

Table of Contents

Preface
Contributors
Abbreviations
1Anaesthetics1
2Cannulation techniques17
3Surgery: selective islet removal and total pancreatectomy37
4Hypophysectomy of fish49
5Surgical extirpation of the Stannius corpuscles: stanniectomy59
6Microsurgery: gonadectomy63
7Hepatectomy77
8Injection techniques85
9Techniques for recording from electric organs93
10Intact and demembranated muscle fibres107
11Isolated perfused fish hearts119
12Methods for assessing kidney and urinary bladder function in fish127
13Cell lines: availability, propagation and isolation145
14Extracorporeal circulation161
15Perfusion of pancreatic islets169
16Perfusion of pancreatic endocrine tissue of teleost fish179
17The isolated rete191
18Teleost pituitary cells: isolation, culture and use205
19Photophores: the analysis of bioluminescent systems215
20Opercular membranes and skin231
21Isolation of gill cells239
22Testes cells: isolation and culture249
23Primary culture of rainbow trout corpuscles of Stannius273
24Isolated cardiac myocytes283
25Oogenesis291
26Adipose tissue305
27Enzymes of lipogenesis313
28Isolation and culture of head kidney macrophages327
29In vitro culture of fish leukocytes341
30Hepatocytes: isolation, maintenance and utilization355
31Organ culture of fish tissues375
32Perfusion of hepatocytes387
33Measurement of intracellular pH in fish hepatocytes399
34Measurement of intracellular calcium in fish liver cells407
35Xenobiotics: designing an in vitro system to study enzymes and metabolism417
36Peptide receptor assays: insulin receptor431
37Thyroid hormone: deiodinase and receptor assays447
38Steroid receptors in fish: membrane and intracellular preparations457
39Membrane, cell preparation and receptor assay - catecholamines469
40Membrane preparation and receptor assays - adenosine477
41Fish mitochondria487
42Peroxisomes and peroxisomal [beta]-oxidation503
43Use of membrane vesicle techniques to characterize nutrient transport processes of the teleost gastrointestinal tract513
44Incorporation of [superscript 35]S-sulfate into branchial cartilage: a biological model to study hormonal regulation of skeletal growth in fish525
45Development of radioimmunoassay for a model peptide hormone: insulin535
46Radioimmunoassay of fish growth hormone, prolactin, and somatolactin545
47Hormone measurement by ELISA: application to fish gonadotrophin557
48Developing an ELISA for a model protein - vitellogenin567
49Bolus-injection of radiolabels in the study of steady state glucose metabolism579
50In vivo nuclear magnetic resonance spectroscopy of aquatic animals591
51Analysis of enzyme regulation via reversible phosphorylation and enzyme binding interactions with macromolecules603
52Metabolite transport in fish red blood cells and hepatocytes615
53Ca[superscript 2+] transport across plasma membranes625
54Analyzing phosphatidylinositol cycling and protein kinase C involvement639
Appendix: Buffered salt solutions, culture media and tissue homogenization buffers649
Species index659
Subject Index663

Read More

Customer Reviews

Average Review:

Write a Review

and post it to your social network

     

Most Helpful Customer Reviews

See all customer reviews >