Analytical Techniques

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Overview

This third volume in the series covers such topics as anaesthetics, cannulation and injection techniques, and surgery. The book will be invaluable to fisheries scientists, aquaculturists, and animal biochemists, physiologists and endocrinologists; it will provide researchers and students with a pertinent information source from theoretical and experimental angles.

This third volume in the series covers such topics as anaesthetics, cannulation and injection techniques, and surgery. The book will be invaluable to fisheries scientists, aquaculturists, and animal biochemists, physiologists and endocrinologists; it will provide researchers and students with a pertinent information source from theoretical and experimental angles.

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Editorial Reviews

From the Publisher
H. Goos
...presents an overwhelming amount of very useful technical information and it will be a really useful guide not only for all researchers in fish physiology. But also for aquaculturists, who will find some useful techniques, such as blood sampling, anaesthetics and operation techniques.
Aquaculture
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Product Details

Table of Contents

Preface
Contributors
Abbreviations
1 Anaesthetics 1
2 Cannulation techniques 17
3 Surgery: selective islet removal and total pancreatectomy 37
4 Hypophysectomy of fish 49
5 Surgical extirpation of the Stannius corpuscles: stanniectomy 59
6 Microsurgery: gonadectomy 63
7 Hepatectomy 77
8 Injection techniques 85
9 Techniques for recording from electric organs 93
10 Intact and demembranated muscle fibres 107
11 Isolated perfused fish hearts 119
12 Methods for assessing kidney and urinary bladder function in fish 127
13 Cell lines: availability, propagation and isolation 145
14 Extracorporeal circulation 161
15 Perfusion of pancreatic islets 169
16 Perfusion of pancreatic endocrine tissue of teleost fish 179
17 The isolated rete 191
18 Teleost pituitary cells: isolation, culture and use 205
19 Photophores: the analysis of bioluminescent systems 215
20 Opercular membranes and skin 231
21 Isolation of gill cells 239
22 Testes cells: isolation and culture 249
23 Primary culture of rainbow trout corpuscles of Stannius 273
24 Isolated cardiac myocytes 283
25 Oogenesis 291
26 Adipose tissue 305
27 Enzymes of lipogenesis 313
28 Isolation and culture of head kidney macrophages 327
29 In vitro culture of fish leukocytes 341
30 Hepatocytes: isolation, maintenance and utilization 355
31 Organ culture of fish tissues 375
32 Perfusion of hepatocytes 387
33 Measurement of intracellular pH in fish hepatocytes 399
34 Measurement of intracellular calcium in fish liver cells 407
35 Xenobiotics: designing an in vitro system to study enzymes and metabolism 417
36 Peptide receptor assays: insulin receptor 431
37 Thyroid hormone: deiodinase and receptor assays 447
38 Steroid receptors in fish: membrane and intracellular preparations 457
39 Membrane, cell preparation and receptor assay - catecholamines 469
40 Membrane preparation and receptor assays - adenosine 477
41 Fish mitochondria 487
42 Peroxisomes and peroxisomal [beta]-oxidation 503
43 Use of membrane vesicle techniques to characterize nutrient transport processes of the teleost gastrointestinal tract 513
44 Incorporation of [superscript 35]S-sulfate into branchial cartilage: a biological model to study hormonal regulation of skeletal growth in fish 525
45 Development of radioimmunoassay for a model peptide hormone: insulin 535
46 Radioimmunoassay of fish growth hormone, prolactin, and somatolactin 545
47 Hormone measurement by ELISA: application to fish gonadotrophin 557
48 Developing an ELISA for a model protein - vitellogenin 567
49 Bolus-injection of radiolabels in the study of steady state glucose metabolism 579
50 In vivo nuclear magnetic resonance spectroscopy of aquatic animals 591
51 Analysis of enzyme regulation via reversible phosphorylation and enzyme binding interactions with macromolecules 603
52 Metabolite transport in fish red blood cells and hepatocytes 615
53 Ca[superscript 2+] transport across plasma membranes 625
54 Analyzing phosphatidylinositol cycling and protein kinase C involvement 639
Appendix: Buffered salt solutions, culture media and tissue homogenization buffers 649
Species index 659
Subject Index 663
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