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Analytical Ultracentrifugation

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This volume contains the contributions of the 10th Symposium on Analytical Ultracentrifugation, held in Regensburg, Germany, on March 13th to 14th, 1997. Topics in the following fields were presented: Technical innovations; innovations in data analysis; modeling; biological systems; ions and polyelectrolytes; polymers, colloids, supramolecular systems; emulsions, gels, and dispersions; interacting systems and assemblies.

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Analytical Ultracentrifugation IV (Progress in Colloid and Polymer Science, Vol. 107). Hardcover, ex-library (private corporate library. Pages clean, binding tight. Tear to ... top of binding. ISBN scraped off back cover. Read more Show Less

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Overview

This volume contains the contributions of the 10th Symposium on Analytical Ultracentrifugation, held in Regensburg, Germany, on March 13th to 14th, 1997. Topics in the following fields were presented: Technical innovations; innovations in data analysis; modeling; biological systems; ions and polyelectrolytes; polymers, colloids, supramolecular systems; emulsions, gels, and dispersions; interacting systems and assemblies.

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Product Details

Table of Contents

Preface
Direct analysis of sedimentation equilibrium distributions reflecting macromolecular interactions 1
Alternative strategies for the characterization of associations in multi-component solutions via measurement of sedimentation equilibrium 11
Non-linear curve-fitting methods for data from the XL-A analytical ultra-centrifuge 20
Rapid molecular mass determination by sedimentation velocity experiments and direct fitting of the concentration profiles 27
A computer program based on the psi function for model-independent analysis of sedimentation equilibrium distributions reflecting macro-molecular interactions 36
Calculation of hydrodynamic parameters of biopolymers from scattering data using whole-body approaches 43
Calculation of hydrodynamic parameters of proteins from crystallographic data using multibody approaches 58
Analytical ultracentrifugation with fluorescence detection and biosafety containment and its application to the prion protein 72
Dye-labelling as a means to study ternary protein complexes by analytical ultracentrifugation: The band 3/ankyrin/aldolase complex from erythrocyte membranes 77
Studies of ligand-mediated conformational changes in enzymes by difference sedimentation velocity in the Optima XL-A ultracentrifuge 82
Quaternary structure and interaction parameters of bovine [alpha]-crystallin: Influence of isolation conditions 88
Investigation of irradiated eye-lens proteins by analytical ultracentrifugation and other techniques 94
Sedimentation analysis of SDS and albumin-SDS complexes 102
The self-association of basic helix-loop-helix peptides 115
Nuclear DNA fractions with grossly different base ratios in the genome of the marine sponge Geodia cydonium 122
Sedimentation equilibrium studies of synthetic polyelectrolytes by means of interference optical methods 127
Investigation of quantum size colloids using the XL-I ultracentrifuge 136
Influence of pressure and solvent composition on the density gradient in the analytical ultracentrifuge. I. Extended Hermans-Ende equation for the equilibrium density gradient 148
Influence of pressure and solvent composition on the density gradient in the analytical ultracentrifuge. II. Direct refractometric determination of the equilibrium and non-equilibrium density gradient 154
First experiences with the new XL-I AUC: Applications in polymer and colloid science 159
Analytical ultracentrifugation as a tool in supramolecular chemistry: A feasibility study using a metal coordination array 166
The sedimentation velocity of a gel 172
New contributions of analytical ultracentrifugation to the investigation of dispersions 180
Determination of hydrodynamic radius: A comparison of ultracentrifuge methods with dynamic light scattering 189
Author Index 193
Subject Index 194
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