Angiogenesis Protocols / Edition 2

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As experimentation and clinical trials with first generation anti-angiogenic agents have yielded results and our understanding of the biology and physiology of blood and lymphatic vessels has increased, a new angiogenesis volume swiftly became a necessity. Angiogenesis Prools, Second Edition remains true to its original vision of providing a single source for angiogenesis researchers, irrespective of levels of resource and expertise, by collecting a range of methods for cell isolation and assessing angiogenesis in vivo or in vitro. This information, however, is expanded to include chapters on circulating endothelial progenitor cells, angiogenic signalling pathways, imaging of angiogenesis, and measurement of tissue blood flow. Written in the Methods in Molecular Biology™ series format, the chapters provide step-by-step laboratory prools, lists of necessary materials and reagents, and a Notes section, which details tips on troubleshooting and avoiding known pitfalls.

Extensive and cutting-edge, Angiogenesis Prools, Second Edition is not only a practical handbook for key techniques, but also an informative and enjoyable read for all those interested, no matter how directly, in angiogenesis.

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Editorial Reviews

Doody's Review Service
Reviewer: Omer Iqbal, MD, FACC, FESC (Loyola University Medical Center)
Description: This second edition of a book on angiogenesis protocols comes at a time when the understanding of the biology and physiology of blood and lymphatic vessels has increased. It presents for angiogenesis researchers a range of methods for cell isolation and assessing angiogenesis in vivo and in vitro besides chapters on circulating endothelial progenitor cells, angiogenic signaling pathways, imaging of angiogenesis and measurement of tissue blood flow. The first edition was published in 2003.
Purpose: This is intended as a single source for angiogenesis researchers, providing a range of methods for cell isolation and assessing angiogenesis in vivo and in vitro. The book is very much needed considering advances in the understanding of the biology and physiology of blood and lymphatic vessels.
Audience: According to the authors, the book is intended not only for angiogenesis researchers, but also for all those interested in angiogenesis.
Features: The first of the book's five sections presents review articles and in two chapters on angiogenic signaling pathways and imaging angiogenesis. Section II covers the microscopic assessment in chapters covering the assessment of tumor angiogenesis in histological samples and immunohistochemical methods for measuring tissue lymphangiogenesis. The four chapters in section III cover cell isolation techniques, including techniques for vascular endothelial cells, lymphatic endothelial cells, vascular smooth muscle cells, and circulating endothelial progenitor cells. The six chapters in section IV on in vitro assessment of angiogenesis explore in vitro assays for endothelial function, in vitro matrigel angiogenesis assay, in vitro angiogenesis in the rat aortic ring model, static and dynamic assays for cell adhesion relevant to the vasculature, genetic manipulation of corneal endothelial cells, and in vitro study of angiogenesis in wound healing. Section V on the in vivo techniques of assessment of angiogenesis has eight chapters covering a wide range of topics such as measurement of angiogenesis phenotype by use of two-dimensional mesenteric angiogenesis assays, quantitative estimation of tissue blood flow, matrigel migration and angiogenesis assay, the sponge model of angiogenesis, and the cranial bone window model besides the corneal pocket assay and studying angiogenesis by intravital microscopy. The book has a number of figures, and the addition of color plates enhances the understanding of the intricate details in angiogenesis. The notes section provides tips on troubleshooting and how to avoid known pitfalls. The index would be expanded to make it easier for readers to locate topics of interest.
Assessment: The second edition is timely, considering the significant advances in the understanding of the biology and physiology of blood and lymphatic vessels since the first edition six years. This is a practical handbook for key techniques in angiogenesis and angiogenesis researchers and others interested in this field will find this book very useful.
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Product Details

  • ISBN-13: 9781588299079
  • Publisher: Springer-Verlag New York, LLC
  • Publication date: 10/24/2008
  • Series: Methods in Molecular Biology Series , #467
  • Edition description: 2nd ed. 2009
  • Edition number: 2
  • Pages: 358
  • Product dimensions: 7.60 (w) x 10.30 (h) x 0.90 (d)

Table of Contents

Section I: Review Articles

Chapter 1. Angiogenic Signalling Pathways
Zubair Ahmed and Roy Bicknell

Chapter 2. Imaging Angiogenesis
Natalie Charnley, Stephanie Donaldson, and Pat Price

Section II: Microscopic Assessment

Chapter 3. Assessing Tumor Angiogenesis in Histological Samples
Stephen B Fox

Chapter 4. Immunohishemical Methods for Measuring Tissue Lymphangiogenesis
Steven Clasper and David G. Jackson

Section III: Cell Isolation Techniques

Chapter 5. Vascular Endothelial Cells from Human Micro- and Macro-vessels: Isolation, Characterisation and Culture
Peter W Hewett

Chapter 6. Lymphatic Endothelial Cells: Establishment of Primaries and Characterization of Established Lines
Riccardo E. Nisato and Michael S. Pepper

Chapter 7. Isolation, Culture and Characterisation of Vascular Smooth Muscle Cells
Adrian T Churchman and Richard CM Siow

Chapter 8. Circulating Endothelial Progenitor Cells (CEPC)
Michael C. Schmid and Judith A. Varner

Section IV: In vitro Techniques

Chapter 9. In vitro Assays for Endothelial Cell Functions Related to Angiogenesis: Proliferation, Motility, Tubular Differentiation and Proteolysis
Suzanne A Eccles, William Court, Lisa Patterson and Sharon Sanderson

Chapter 10. Tube Formation: an In Vitro Matrigel Angiogenesis Assay
M. Lourdes Ponce

Chapter 11. Three-dimensional In vitro Angiogenesis in the Rat Aortic Ring Model
David C. West and Mike F. Burbridge

Chapter 12. Static and Dynamic Assays of Cell Adhesion Relevant to the Vasculature
Lynn M. Butler, Helen M. McGettrick, and Gerard B. Nash

Chapter 13. Genetic Manipulation of Corneal Endothelial Cells: Transfection and Viral Transduction
Eckart Bertelmann

Chapter 14. Creation of Human Skin Equivalents for the In vitro Study of Angiogenesis in Wound Healing
Ira M. Herman and Alice Leung

Section V: In vivo Techniques

Chapter 15. Measurement of Angiogenic Phenotype by Use of a Two Dimensional Mesenteric Angiogenesis Assay
Andrew V Benest and David O Bates

Chapter 16. Quantitative Estimation of Tissue Blood Flow Rate
Gillian M Tozer, Vivien E Prise and Vincent J Cunningham

Chapter 17. In Vivo Matrigel Migration and Angiogenesis Assay
Katherine M. Malinda

Chapter 18. The Sponge Implant Model of Angiogenesis
Silvia Passos Andrade and Mônica Alves Neves Diniz Ferreira

Chapter 19. The Dorsal Skinfold Chamber: Studying Angiogenesis by Intravital Microscopy
Axel Sckell and Michael Leunig

Chapter 20. The Corneal Pocket Assay
Marina Ziche and Lucia Morbidelli

Chapter 21. Use of the Hollow Fibre Assay for Studies on Tumor Neovasculature
Steven D. Shnyder

Chapter 22. The Cranial Bone Window Model: Studying Angiogenesis of Primary and Secondary Bone Tumors by Intravital Microscopy
Axel Sckell and Frank M. Klenke

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