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Doody's Review ServiceReviewer: Omer Iqbal, MD, FACC, FESC (Loyola University Medical Center)
Description: This second edition of a book on angiogenesis protocols comes at a time when the understanding of the biology and physiology of blood and lymphatic vessels has increased. It presents for angiogenesis researchers a range of methods for cell isolation and assessing angiogenesis in vivo and in vitro besides chapters on circulating endothelial progenitor cells, angiogenic signaling pathways, imaging of angiogenesis and measurement of tissue blood flow. The first edition was published in 2003.
Purpose: This is intended as a single source for angiogenesis researchers, providing a range of methods for cell isolation and assessing angiogenesis in vivo and in vitro. The book is very much needed considering advances in the understanding of the biology and physiology of blood and lymphatic vessels.
Audience: According to the authors, the book is intended not only for angiogenesis researchers, but also for all those interested in angiogenesis.
Features: The first of the book's five sections presents review articles and in two chapters on angiogenic signaling pathways and imaging angiogenesis. Section II covers the microscopic assessment in chapters covering the assessment of tumor angiogenesis in histological samples and immunohistochemical methods for measuring tissue lymphangiogenesis. The four chapters in section III cover cell isolation techniques, including techniques for vascular endothelial cells, lymphatic endothelial cells, vascular smooth muscle cells, and circulating endothelial progenitor cells. The six chapters in section IV on in vitro assessment of angiogenesis explore in vitro assays for endothelial function, in vitro matrigel angiogenesis assay, in vitro angiogenesis in the rat aortic ring model, static and dynamic assays for cell adhesion relevant to the vasculature, genetic manipulation of corneal endothelial cells, and in vitro study of angiogenesis in wound healing. Section V on the in vivo techniques of assessment of angiogenesis has eight chapters covering a wide range of topics such as measurement of angiogenesis phenotype by use of two-dimensional mesenteric angiogenesis assays, quantitative estimation of tissue blood flow, matrigel migration and angiogenesis assay, the sponge model of angiogenesis, and the cranial bone window model besides the corneal pocket assay and studying angiogenesis by intravital microscopy. The book has a number of figures, and the addition of color plates enhances the understanding of the intricate details in angiogenesis. The notes section provides tips on troubleshooting and how to avoid known pitfalls. The index would be expanded to make it easier for readers to locate topics of interest.
Assessment: The second edition is timely, considering the significant advances in the understanding of the biology and physiology of blood and lymphatic vessels since the first edition six years. This is a practical handbook for key techniques in angiogenesis and angiogenesis researchers and others interested in this field will find this book very useful.