Animal Cell Biotechnology: Methods and Protocols / Edition 2

Animal Cell Biotechnology: Methods and Protocols / Edition 2

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by Ralf Portner
     
 

Animal Cell Biotechnology: Methods and Protocols covers new techniques for setting up mammalian cell lines for production of biopharmaceuticals, and optimizing critical parameters for cell culture considering the whole cascade from the lab to the final production. This volume is divided into six parts, each reflecting the process required for differenct stages

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Overview

Animal Cell Biotechnology: Methods and Protocols covers new techniques for setting up mammalian cell lines for production of biopharmaceuticals, and optimizing critical parameters for cell culture considering the whole cascade from the lab to the final production. This volume is divided into six parts, each reflecting the process required for differenct stages of production.

Product Details

ISBN-13:
9781588296603
Publisher:
Springer-Verlag New York, LLC
Publication date:
04/28/2007
Series:
Methods in Biotechnology Series, #24
Edition description:
2nd ed. 2007
Pages:
512
Product dimensions:
6.20(w) x 9.30(h) x 1.10(d)

Meet the Author

Table of Contents


Preface     v
Contributors     xiii
Basic Techniques for Establishing Production Cell Lines
Transduction Technologies   David A. Fluri   Martin Fussenegger     3
Cells for Gene Therapy and Vector Production   Christophe Delenda   Miguel Chillon   Anne-Marie Douar   Otto-Wilhelm Merten     23
Technology and Production of Murine Monoclonal and Recombinant Antibodies and Antibody Fragments   Alexandra Dorn-Beineke   Stefanie Nittka   Michael Neumaier     93
DNA Fingerprinting and Characterization of Animal Cell Lines   Glyn N. Stacey   Ed Byrne   J. Ross Hawkins     123
Basic Cultivation Techniques
Microcarrier Cell Culture Technology   Gerald Bluml     149
Cell Encapsulation   Holger Hubner     179
Tools for High-Throughput Medium and Process Optimization   Martin Jordan   Nigel Jenkins     193
Cell Characterization and Analysis
Cell Counting and Viability Measurements   Michael Butler   Maureen Spearman     205
Monitoring of Growth, Physiology, and Productivity of Animal Cells by Flow Cytometry   Silvia Carroll   Mariam Naeiri   Mohamed Al-Rubeai     223
NuclearMagnetic Resonance Methods for Monitoring Cell Growth and Metabolism in Intensive Bioreactors   Andre A. Neves   Kevin M. Brindle     239
Methods for Off-Line Analysis of Nutrients and Products in Mammalian Cell Culture   Heino Buntemeyer     253
Application of Stoichiometric and Kinetic Analyses to Characterize Cell Growth and Product Formation   Derek Adams   Rashmi Korke   Wei-Shou Hu     269
Measurement of Apoptosis in Cell Culture   Adiba Ishaque   Mohamed Al-Rubeai     285
Metabolic Flux Estimation in Mammalian Cell Cultures   Chetan T. Goudar   Richard Biener   James M. Piret   Konstantin B. Konstantinov     301
Special Cultivation Techniques
Disposable Bioreactors for Inoculum Production and Protein Expression   Regine Eibl   Dieter Eibl     321
Hollow Fiber Cell Culture   John M. Davis     337
Cultivation of Mammalian Cells in Fixed-Bed Reactors   Ralf Portner   Oscar B. J. Platas Barradas     353
Configuration of Bioreactors   Dirk E. Martens   Evert Jan van den End     371
Downstream Techniques
Membrane Filtration in Animal Cell Culture   Peter Czermak   Dirk Nehring   Ranil Wickramasinghe      397
Chromatographic Techniques in the Downstream Processing of (Recombinant) Proteins   Ruth Freitag     421
Special Applications
Vaccine Production: State of The Art and Future Needs in Upstream Processing   Yvonne Genzel   Udo Reichl     457
Retrovirus Production and Characterization   Pedro E. Cruz   Marlene Carmo   Teresa Rodrigues   Paula Alves     475
Insect Cell Cultivation and Generation of Recombinant Baculovirus Particles for Recombinant Protein Production   Sabine Geisse     489
Index     509

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