Animal Cell Biotechnology: Methods and Protocols

Overview

Animal Cell Biotechnology: Methods and Prools, Third Edition constitutes a comprehensive manual of state-of-the-art and new techniques for setting up mammalian cell lines for production of biopharmaceuticals, and for optimizing critical parameters for cell culture from lab to final production. The volume is divided into five parts that reflect the processes required for different stages of production. In Part I, basic techniques for establishment of production cell lines are addressed, especially high-throughput ...

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Overview

Animal Cell Biotechnology: Methods and Prools, Third Edition constitutes a comprehensive manual of state-of-the-art and new techniques for setting up mammalian cell lines for production of biopharmaceuticals, and for optimizing critical parameters for cell culture from lab to final production. The volume is divided into five parts that reflect the processes required for different stages of production. In Part I, basic techniques for establishment of production cell lines are addressed, especially high-throughput synchronization, insect cell lines, transient gene and protein expression, DNA Profiling and Characterisation. Part II addresses tools for process and medium optimization as well as microcarrier technology while Part III covers monitoring of cell growth, viability and apoptosis, metabolic flux estimation, quenching methods as well as NMR-based techniques. Part IV details cultivation techniques, and Part V describes special applications, including vaccine production, baculovirus protein expression, chromatographic techniques for downstream as well as membrane techniques for virus separation. Written in the successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible prools, and notes on troubleshooting and avoiding known pitfalls.

Animal Cell Biotechnology: Methods and Prools, Third Edition provides a compendium of techniques for scientists in industrial and research laboratories that use mammalian cells for biotechnology purposes.

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Product Details

  • ISBN-13: 9781627037327
  • Publisher: Springer-Verlag New York, LLC
  • Publication date: 12/16/2013
  • Series: Methods in Molecular Biology Series , #1104
  • Edition description: 3rd ed. 2014
  • Edition number: 3
  • Pages: 498
  • Product dimensions: 7.00 (w) x 10.00 (h) x 1.13 (d)

Table of Contents

Part I. Cell Line Development and Selection

1. High-Throughput Synchronization of Mammalian Cell Cultures by Spiral Microfluidic Sorter

Wong Cheng Lee, Ali Asgar. S. Bhagat, and Chwee Teck Lim

2. Insect Cell Line Development Using Flp-Mediated Cassette Exchange Technology

João Vidigal, Fabiana Fernandes, Ana S Coroadinha, Ana P Teixeira, and Paula M. Alves

3. Scalable Transient Gene Expression in Adherent Mammalian Cells Using Polyethylenimine

Lukas Fliedl and Christian Kaisermayer

4. Scalable Transient Protein Expression

Henry C. Chiou, Sanjay Vasu, Chao Yan Liu, Isabel Cisneros, Meredith B. Jones, and Jon Zmuda

5. DNA Profiling and Characterisation of Animal Cell Lines

Glyn N. Stacey, Ed Byrne, and J. Ross Hawkins

Part II. Techniques for Process Development

6. Tools for High-Throughput Process and Medium Optimization

Martin Jordan and Matthieu Stettler

7. Designing Media for Animal Cell Culture – CHO Cells, The Industrial Standard

Karlheinz Landauer

8. Feed Optimization in Fed-Batch Culture

Ana Rita Costa, Maria Elisa Rodrigues, Mariana Henriques, Rosário Oliveira, and Joana Azeredo

9. Screening and Optimization of Chemically-Defined Media and Feeds with Integrated and Statistical Approaches

Zhihua Xiao, Michelle Sabourin, Graziella Piras, and Stephen Gorfien

10. Evaluation of Solid and Porous Microcarriers for Cell Growth and Production of Recombinant Proteins

Maria Elisa Rodrigues, Ana Rita Costa, Mariana Henriques, Joana Azeredo, and Rosário Oliveira

11. Microbioreactors and Scale-Down Models: Growth of CHO Cells Using the Pall Micro24 MicroReactor System

Steve R.C. Warr

Part III. Cell Characterisation and Analysis

12. Monitoring Cell Growth, Viability and Apoptosis

Michael Butler, Maureen Spearman, and Katrin Braasch

13. Metabolic Flux Estimation in Mammalian Cell Cultures

Chetan T. Goudar, Richard K. Biener, James M. Piret, and Konstantin B. Konstantinov

14. Quenching Methods for the Analysis of Intracellular Metabolites

Judith Wahrheit and Elmar Heinzle

15. NMR Methods for Metabolomics of Mammalian Cell Culture Bioreactors

Nelly Aranibar and Michael D. Reily

16. 1H-NMR Prool for Exometabolome Analysis of Cultured Mammalian Cells

Tiago M. Duarte, Nuno Carinhas, Ana Carina Silva, Paula M. Alves, and Ana P. Teixeira

Part IV. Cultivation Techniques

17. Microfluidic Perfusion Culture

Koji Hattori, Shinji Sugiura, and Toshiyuki Kanamori

18. Disposable Bioreactors for Inoculum Production and Protein Expression Regine Eibl, Christian Löffelholz, and Dieter Eibl

19. Configuration of Bioreactors

Dirk E. Martens, E. J. van den End, and Mathieu Streefland

20. Process Analytical Technology (PAT) in Insect and Mammalian Cell Culture Processes – Dielectric Spectroscopy and Focused Beam Reflectance Measurement (FBRM)

Damir Druzinec, Katja Weiss, Christiane Elseberg, Denise Salzig, Matthias Kraume, Ralf Pörtner, and Peter Czermak

21. Cell Assessment by Atline Microscopy

Alexander Babitzky, Patrick Lindner, and Thomas Scheper

22. Seed Train Optimization for Cell Culture

Björn Frahm

Part V. Special Applications

23. Vaccine Production - Upstream Processing with Adherent or Suspension Cell Lines

Yvonne Genzel, Jana Rödig, Erdmann Rapp, and Udo Reichl

24. Efficient Processes for Protein Expression Using Recombinant Baculovirus Particles

Heike Cremer, Ingrid Bechtold, Marion Mahnke, and René Assenberg

25. Chromatographic Techniques in the Downstream Processing of Proteins in Biotechnology

Ruth Freitag

26. Virus Separation Using Membranes

Tanja A. Grein, Ronald Michalsky, and Peter Czermak

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