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Animal Cell Biotechnology: Methods and Protocols / Edition 3
     

Animal Cell Biotechnology: Methods and Protocols / Edition 3

by Ralf Portner
 

ISBN-10: 1627037322

ISBN-13: 9781627037327

Pub. Date: 12/16/2013

Publisher: Springer-Verlag New York, LLC

Animal Cell Biotechnology: Methods and Protocols, Second Edition constitutes a comprehensive manual of state-of-the-art and new techniques for setting up mammalian cell lines for production of biopharmaceuticals, and for optimizing critical parameters for cell culture considering the whole cascade from lab to final production. The volume is divided into five parts

Overview

Animal Cell Biotechnology: Methods and Protocols, Second Edition constitutes a comprehensive manual of state-of-the-art and new techniques for setting up mammalian cell lines for production of biopharmaceuticals, and for optimizing critical parameters for cell culture considering the whole cascade from lab to final production. The volume is divided into five parts that reflect the processes required for different stages of production. In Part I, basic techniques for establishment of production cell lines are addressed, especially transduction techniques, cells for gene therapy, and antibody production. Part II addresses basic cultivation techniques, such as microcarrier culture and encapsulation.

Part III covers cell characterization and analysis, including flow cytometric applications, NMR-based techniques, and biochemical and cytometric techniques. Part IV details cultivation techniques, such as disposable bioreactors, hollow fiber cell culture, fixed bed reactors, and configuration of bioreactors. Part V covers downstream techniques such as membrane filtration techniques, while Part VI describes special applications, including retroviral vectors.

Animal Cell Biotechnology: Methods and Protocols, Second Edition provides a compendium of techniques for scientists in industrial and research laboratories that use mammalian cells for biotechnology purposes. Features: Chapters written by world-renowned experts, Details novel techniques for basic cultivation and complex techniques such as disposable bioreactors, hollow fiber cell culture, and fixed bed reactors, Provides a state-of-the-art manual for setting up mammalian cell lines for production of biopharmaceuticals, Divided intosections to mimic the processes required for different stages of production.

Product Details

ISBN-13:
9781627037327
Publisher:
Springer-Verlag New York, LLC
Publication date:
12/16/2013
Series:
Methods in Molecular Biology Series , #1104
Edition description:
3rd ed. 2014
Pages:
498
Product dimensions:
7.01(w) x 10.00(h) x 0.04(d)

Table of Contents


Preface     v
Contributors     xiii
Basic Techniques for Establishing Production Cell Lines
Transduction Technologies   David A. Fluri   Martin Fussenegger     3
Cells for Gene Therapy and Vector Production   Christophe Delenda   Miguel Chillon   Anne-Marie Douar   Otto-Wilhelm Merten     23
Technology and Production of Murine Monoclonal and Recombinant Antibodies and Antibody Fragments   Alexandra Dorn-Beineke   Stefanie Nittka   Michael Neumaier     93
DNA Fingerprinting and Characterization of Animal Cell Lines   Glyn N. Stacey   Ed Byrne   J. Ross Hawkins     123
Basic Cultivation Techniques
Microcarrier Cell Culture Technology   Gerald Bluml     149
Cell Encapsulation   Holger Hubner     179
Tools for High-Throughput Medium and Process Optimization   Martin Jordan   Nigel Jenkins     193
Cell Characterization and Analysis
Cell Counting and Viability Measurements   Michael Butler   Maureen Spearman     205
Monitoring of Growth, Physiology, and Productivity of Animal Cells by Flow Cytometry   Silvia Carroll   Mariam Naeiri   Mohamed Al-Rubeai     223
NuclearMagnetic Resonance Methods for Monitoring Cell Growth and Metabolism in Intensive Bioreactors   Andre A. Neves   Kevin M. Brindle     239
Methods for Off-Line Analysis of Nutrients and Products in Mammalian Cell Culture   Heino Buntemeyer     253
Application of Stoichiometric and Kinetic Analyses to Characterize Cell Growth and Product Formation   Derek Adams   Rashmi Korke   Wei-Shou Hu     269
Measurement of Apoptosis in Cell Culture   Adiba Ishaque   Mohamed Al-Rubeai     285
Metabolic Flux Estimation in Mammalian Cell Cultures   Chetan T. Goudar   Richard Biener   James M. Piret   Konstantin B. Konstantinov     301
Special Cultivation Techniques
Disposable Bioreactors for Inoculum Production and Protein Expression   Regine Eibl   Dieter Eibl     321
Hollow Fiber Cell Culture   John M. Davis     337
Cultivation of Mammalian Cells in Fixed-Bed Reactors   Ralf Portner   Oscar B. J. Platas Barradas     353
Configuration of Bioreactors   Dirk E. Martens   Evert Jan van den End     371
Downstream Techniques
Membrane Filtration in Animal Cell Culture   Peter Czermak   Dirk Nehring   Ranil Wickramasinghe      397
Chromatographic Techniques in the Downstream Processing of (Recombinant) Proteins   Ruth Freitag     421
Special Applications
Vaccine Production: State of The Art and Future Needs in Upstream Processing   Yvonne Genzel   Udo Reichl     457
Retrovirus Production and Characterization   Pedro E. Cruz   Marlene Carmo   Teresa Rodrigues   Paula Alves     475
Insect Cell Cultivation and Generation of Recombinant Baculovirus Particles for Recombinant Protein Production   Sabine Geisse     489
Index     509

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