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From The CriticsReviewer: Bruce A. Fenderson, PhD (Thomas Jefferson University)
Description: Cell culture is widely used as a model system for research in the life sciences. For example, embryonic stem cells are cultured for preclinical studies in regenerative medicine. This book summarizes state-of-the art methods for analyzing the growth and behavior of cells in culture. Its 11 chapters cover topics ranging from biological safety to light microscopy to optimization of serum-free media. The primary focus is on modern cell biology and biochemistry.
Purpose: According to the editor, the purpose is to provide a "useful and concise introduction to the field" and a handy source of protocols and references to "keep the laboratory running smoothly." A working knowledge of cell biology and biochemistry is assumed. The authors are established researchers with considerable practical experience.
Audience: The book is written for basic science and clinical researchers in both academic institutions and pharmaceutical industry. The broad range of topics and detailed information will also appeal to scientists and technicians interested in cell engineering and biotechnology.
Features: Each chapter provides an introduction, discussion of goal-oriented procedures, practical tips, exciting examples of research findings, and an extensive list of references. Key concepts and claims are documented. The book is carefully edited and easy to read, providing a list of commonly used abbreviations and symbols. The black-and-white illustrations are well done, highlighting essential methods and key points.
Assessment: This softcover book will find a home in the laboratory. It is more than a recipe book, providing background information on principles and innovative approaches. It brings together the collected knowledge and wisdom of nearly four decades of experience using animal cells in culture and presents essential methods that are tried and tested. It will be a great resource for students and established researchers who want to learn more about modern cell culture techniques.