Animal Cell Culture: Essential Methods / Edition 1

Paperback (Print)
Used and New from Other Sellers
Used and New from Other Sellers
from $55.43
Usually ships in 1-2 business days
(Save 24%)
Other sellers (Paperback)
  • All (6) from $55.43   
  • New (5) from $55.43   
  • Used (1) from $55.43   


This is a comprehensive research guide that describes both the key new techniques and more established methods. Every chapter discusses the merits and limitations of the various approaches and then provides selected tried-and-tested protocols, as well as a plethora of good practical advice, for immediate use at the bench. It presents the most accessible and comprehensive introduction available to the culture and experimental manipulation of animal cells. Detailed protocols for a wide variety of methods provide the core of each chapter, making new methodology easily accessible. This book is an essential laboratory manual for all undergraduates and graduates about to embark on a cell culture project. It is a book which both experienced researchers and those new to the field will find invaluable.

Read More Show Less

Editorial Reviews

From the Publisher
"It will be a great resource for students and established researchers who want to learn more about modern cell culture techniques." (Doody's, 13 January 2012)
Doody's Review Service
Reviewer: Bruce A. Fenderson, PhD (Thomas Jefferson University)
Description: Cell culture is widely used as a model system for research in the life sciences. For example, embryonic stem cells are cultured for preclinical studies in regenerative medicine. This book summarizes state-of-the art methods for analyzing the growth and behavior of cells in culture. Its 11 chapters cover topics ranging from biological safety to light microscopy to optimization of serum-free media. The primary focus is on modern cell biology and biochemistry.
Purpose: According to the editor, the purpose is to provide a "useful and concise introduction to the field" and a handy source of protocols and references to "keep the laboratory running smoothly." A working knowledge of cell biology and biochemistry is assumed. The authors are established researchers with considerable practical experience.
Audience: The book is written for basic science and clinical researchers in both academic institutions and pharmaceutical industry. The broad range of topics and detailed information will also appeal to scientists and technicians interested in cell engineering and biotechnology.
Features: Each chapter provides an introduction, discussion of goal-oriented procedures, practical tips, exciting examples of research findings, and an extensive list of references. Key concepts and claims are documented. The book is carefully edited and easy to read, providing a list of commonly used abbreviations and symbols. The black-and-white illustrations are well done, highlighting essential methods and key points.
Assessment: This softcover book will find a home in the laboratory. It is more than a recipe book, providing background information on principles and innovative approaches. It brings together the collected knowledge and wisdom of nearly four decades of experience using animal cells in culture and presents essential methods that are tried and tested. It will be a great resource for students and established researchers who want to learn more about modern cell culture techniques.
Read More Show Less

Product Details

  • ISBN-13: 9780470666586
  • Publisher: Wiley
  • Publication date: 5/10/2011
  • Series: Essential Methods Series , #1
  • Edition number: 1
  • Pages: 374
  • Product dimensions: 6.60 (w) x 9.60 (h) x 0.80 (d)

Meet the Author

Dr John Davis: Visiting Lecturer in Biotechnology, University of Hertfordshire, previously Senior Project Scientist at Bio Products Laboratory, UK.

Read More Show Less

Table of Contents





1 The Cell Culture Laboratory (Sue Clarke and Janette Dillon).

2 Sterilization (Peter L. Roberts).

3 Microscopy of Living Cells (Colin Gray and Daniel Zicha).

4 Basic Techniques and Media, the Maintenance of Cell Lines, and Safety (John M. Davis).

5 Development and Optimization of Serum- and Protein-free Culture Media (Stephen F. Gorfien and David W. Jayme).

6 Cryopreservation and Banking of Cell Lines (Glyn N. Stacey, Ross Hawkins and Roland A. Fleck).

7 Primary Culture of Specific Cell Types and the Establishment of Cell Lines (Kee Woei Ng, Mohan Chothirakottu Abraham, David Tai Wei Leong, Chris Morris and Jan-Thorsten Schantz).

8 Cloning (John Clarke, Alison Porter and John M. Davis).

9 The Quality Control of Animal Cell Lines and the Prevention, Detection and Cure of Contamination (Peter Thraves and Cathy Rowe).

10 Systems for Cell Culture Scale-up (Jennifer Halsall and John M. Davis).

11 Good Laboratory Practice in the Cell Culture Laboratory (Barbara Orton).


Colour plates.

Read More Show Less

Customer Reviews

Be the first to write a review
( 0 )
Rating Distribution

5 Star


4 Star


3 Star


2 Star


1 Star


Your Rating:

Your Name: Create a Pen Name or

Barnes & Review Rules

Our reader reviews allow you to share your comments on titles you liked, or didn't, with others. By submitting an online review, you are representing to Barnes & that all information contained in your review is original and accurate in all respects, and that the submission of such content by you and the posting of such content by Barnes & does not and will not violate the rights of any third party. Please follow the rules below to help ensure that your review can be posted.

Reviews by Our Customers Under the Age of 13

We highly value and respect everyone's opinion concerning the titles we offer. However, we cannot allow persons under the age of 13 to have accounts at or to post customer reviews. Please see our Terms of Use for more details.

What to exclude from your review:

Please do not write about reviews, commentary, or information posted on the product page. If you see any errors in the information on the product page, please send us an email.

Reviews should not contain any of the following:

  • - HTML tags, profanity, obscenities, vulgarities, or comments that defame anyone
  • - Time-sensitive information such as tour dates, signings, lectures, etc.
  • - Single-word reviews. Other people will read your review to discover why you liked or didn't like the title. Be descriptive.
  • - Comments focusing on the author or that may ruin the ending for others
  • - Phone numbers, addresses, URLs
  • - Pricing and availability information or alternative ordering information
  • - Advertisements or commercial solicitation


  • - By submitting a review, you grant to Barnes & and its sublicensees the royalty-free, perpetual, irrevocable right and license to use the review in accordance with the Barnes & Terms of Use.
  • - Barnes & reserves the right not to post any review -- particularly those that do not follow the terms and conditions of these Rules. Barnes & also reserves the right to remove any review at any time without notice.
  • - See Terms of Use for other conditions and disclaimers.
Search for Products You'd Like to Recommend

Recommend other products that relate to your review. Just search for them below and share!

Create a Pen Name

Your Pen Name is your unique identity on It will appear on the reviews you write and other website activities. Your Pen Name cannot be edited, changed or deleted once submitted.

Your Pen Name can be any combination of alphanumeric characters (plus - and _), and must be at least two characters long.

Continue Anonymously

    If you find inappropriate content, please report it to Barnes & Noble
    Why is this product inappropriate?
    Comments (optional)