Animal Cell Culture: Essential Methods / Edition 1

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Overview

This is a comprehensive research guide that describes both the key new techniques and more established methods. Every chapter discusses the merits and limitations of the various approaches and then provides selected tried-and-tested protocols, as well as a plethora of good practical advice, for immediate use at the bench. It presents the most accessible and comprehensive introduction available to the culture and experimental manipulation of animal cells. Detailed protocols for a wide variety of methods provide the core of each chapter, making new methodology easily accessible.
This book is an essential laboratory manual for all undergraduates and graduates about to embark on a cell culture project. It is a book which both experienced researchers and those new to the field will find invaluable.

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Editorial Reviews

From the Publisher
"It will be a great resource for students and established researchers who want to learn more about modern cell culture techniques." (Doody's, 13 January 2012)
Doody's Review Service
Reviewer: Bruce A. Fenderson, PhD (Thomas Jefferson University)
Description: Cell culture is widely used as a model system for research in the life sciences. For example, embryonic stem cells are cultured for preclinical studies in regenerative medicine. This book summarizes state-of-the art methods for analyzing the growth and behavior of cells in culture. Its 11 chapters cover topics ranging from biological safety to light microscopy to optimization of serum-free media. The primary focus is on modern cell biology and biochemistry.
Purpose: According to the editor, the purpose is to provide a "useful and concise introduction to the field" and a handy source of protocols and references to "keep the laboratory running smoothly." A working knowledge of cell biology and biochemistry is assumed. The authors are established researchers with considerable practical experience.
Audience: The book is written for basic science and clinical researchers in both academic institutions and pharmaceutical industry. The broad range of topics and detailed information will also appeal to scientists and technicians interested in cell engineering and biotechnology.
Features: Each chapter provides an introduction, discussion of goal-oriented procedures, practical tips, exciting examples of research findings, and an extensive list of references. Key concepts and claims are documented. The book is carefully edited and easy to read, providing a list of commonly used abbreviations and symbols. The black-and-white illustrations are well done, highlighting essential methods and key points.
Assessment: This softcover book will find a home in the laboratory. It is more than a recipe book, providing background information on principles and innovative approaches. It brings together the collected knowledge and wisdom of nearly four decades of experience using animal cells in culture and presents essential methods that are tried and tested. It will be a great resource for students and established researchers who want to learn more about modern cell culture techniques.
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Product Details

  • ISBN-13: 9780470666586
  • Publisher: Wiley
  • Publication date: 5/10/2011
  • Series: Essential Methods Series , #1
  • Edition number: 1
  • Pages: 374
  • Product dimensions: 6.60 (w) x 9.60 (h) x 0.80 (d)

Meet the Author

Dr John Davis: Visiting Lecturer in Biotechnology, University of Hertfordshire, previously Senior Project Scientist at Bio Products Laboratory, UK.

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Table of Contents

Contributors.

Preface.

Abbreviations.

Protocols.

1 The Cell Culture Laboratory (Sue Clarke and Janette Dillon).

2 Sterilization (Peter L. Roberts).

3 Microscopy of Living Cells (Colin Gray and Daniel Zicha).

4 Basic Techniques and Media, the Maintenance of Cell Lines, and Safety (John M. Davis).

5 Development and Optimization of Serum- and Protein-free Culture Media (Stephen F. Gorfien and David W. Jayme).

6 Cryopreservation and Banking of Cell Lines (Glyn N. Stacey, Ross Hawkins and Roland A. Fleck).

7 Primary Culture of Specific Cell Types and the Establishment of Cell Lines (Kee Woei Ng, Mohan Chothirakottu Abraham, David Tai Wei Leong, Chris Morris and Jan-Thorsten Schantz).

8 Cloning (John Clarke, Alison Porter and John M. Davis).

9 The Quality Control of Animal Cell Lines and the Prevention, Detection and Cure of Contamination (Peter Thraves and Cathy Rowe).

10 Systems for Cell Culture Scale-up (Jennifer Halsall and John M. Davis).

11 Good Laboratory Practice in the Cell Culture Laboratory (Barbara Orton).

Index.

Colour plates.

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