Antibody Phage Display: Methods and Protocols / Edition 2

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Since its introduction almost 20 years ago, phage display technology has revolutionized approaches to the analysis of biomedical problems, quickly impacting the fields of immunology, cell biology, biotechnology, pharmacology, and drug discovery. In Antibody Phage Display: Methods and Protocols, Second Edition, expert researchers explore the latest in this cutting-edge technology, providing an invaluable resource that will guide readers in the design and execution of experiments based around antibody phage display. Chapters present a wide range of methods of isolating recombinant antibodies from phage display libraries, examine how the targets recognized by antibodies of interest can be identified, discuss the identification and exploitation of antibodies that can enter cells and bind to cytosolic targets, and include novel approaches to the expression of recombinant antibodies. Composed in the highly successful Methods in Molecular BiologyT series format, each chapter contains a brief introduction, step-by-step methods, a list of necessary materials, and a Notes section which shares tips on troubleshooting and avoiding known pitfalls. Detailed and innovative, Antibody Phage Display: Methods and Protocols, Second Edition is a critical handbook on phage display technology which is certain to stimulate the reader's imagination as much as it will guide future practice in the laboratory.

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Editorial Reviews

Doody's Review Service
Reviewer: Marion C. Cohen, PhD (SUNY Downstate Medical Center)
Description: Phage display technology has had an important impact on immunology, cell biology, biotechnology, pharmacology, and drug discovery. This book presents a wide range of methods of isolating recombinant antibodies from phage display libraries, examining how the targets of these antibodies can be identified, and how they can be used. Each chapter is presented in the standard style of the Methods in Molecular Biology series and includes a brief introduction, step-by-step methods, a list of materials and reagents, and finally a notes section that describes ways of troubleshooting problems as well as potential pitfalls. The first edition was published in 2002.
Purpose: The goal of both editions is to provide a resource to help investigators design and execute experiments based on antibody phage display techniques. A second aim is to suggest possibilities that will lead others to consider how they might use this technology for their particular research problems. Given the innovations that have occurred in the use of phage display, a second edition is certainly warranted.
Audience: This is clearly a book meant for individuals in research laboratories. However, those researchers need not only be those interested in basic research; they could also include those who are focused on novel therapeutic antibodies for clinical applications. The editor clearly intends to provide a resource for innovative investigators to help them use this methodology to answer their questions. He is an expert in this technique, as are the authors of the individual chapters.
Features: Although there are probably some who would quibble about a lack of a specific fact in some assay, the overall approach to experimental design is very detailed. At the least, if one has a problem with a technique, it should be possible to contact the author(s) for some help. The choice of topics is based on the idea that among the wide range of methods presented, there is an approach that will be suitable for most questions. Nonetheless, it is always possible that there is something that should have been included, but that does not detract from the utility of the book for most researchers. As always, the very detailed experimental methods and the notes section with its caveats are the high points of each chapter.
Assessment: There are many research articles and reviews describing the methodology involved in phage display technology, but few give the level of procedural detail found in each chapter of this book. The ability to compare diverse assays in one volume is likely to be particularly helpful to someone who is new to these protocols. Finally, the description of different assays in terms of their applications may suggest new directions for some investigators.
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Product Details

  • ISBN-13: 9781617794933
  • Publisher: Springer-Verlag New York, LLC
  • Publication date: 12/1/2011
  • Series: Methods in Molecular Biology Series, #562
  • Edition description: Softcover reprint of hardcover 2nd ed. 2009
  • Edition number: 2
  • Pages: 240
  • Sales rank: 1,206,545
  • Product dimensions: 7.66 (w) x 10.25 (h) x 0.56 (d)

Table of Contents

Table of Contents


Antibody phage display – overview of a powerful technology that has quickly translated to the clinic

Beatrix Kotlan and Mark C. Glassy

Design and construction of synthetic phage-displayed Fab libraries

Jenny Bostrom and Germaine Fuh

Affinity isolation of antigen-specific circulating B cells for generation of phage display-derived human monoclonal antibodies

Henrik J. Ditzel

Construction of phage antibody repertoires from the blood of West Nile virus infected donors

Mark Throsby and John de Kruif

Anti-ß2GP-I and anti-prothrombin antibodies generated by phage display

Javier Cabiedes and Antonio R. Cabral

The generation and selection of single-domain, V region libraries from nurse sharks

Martin F. Flajnik and Helen Dooley

The isolation of scFvs against small target molecules

Iain Shaw and Marian Kane

Display and selection of scFv antibodies on HEK-293T cells

Mitchell Ho and Ira Pastan

Isolation of scFvs that inhibit the NS3 protease of Hepatitis C virus by a combination of phage display and a bacterial genetic screen

Limor Nahary, Alla Trahtenherts and Itai Benhar

Guided selection methods through chain shuffling

Bao Guo-qiang and He Xian-Li

Sequential antigen panning for selection of broadly cross-reactive HIV-1 neutralizing human monoclonal antibodies

Mei-Yun Zhang and Dimiter S. Dimitrov

Selection of antibodies able to rapidly enter mammalian cells

Marie-Alix Poul

Identification of the specificity of isolated phage display single chain antibodies using yeast 2-hybrid screens

Nicolaj Rasmussen and Henrik J.Ditzel

Generation of bispecific and tandem diabodies

Sergey M. Kipriyanov

Intrabody expression in eukaryotic cells

Laurence Guglielmi and Pierre Martineau

High level periplasmic expression and purification of scFvs

Sergey M. Kipriyanov

Expression of single-chain Fv fragments in E. coli cytoplasm

Laurence Guglielmi and Pierre Martineau

High-level expression of a phage display-derived scFv in Pichia pastoris

Leonardo M. Damasceno, Frank Lee, Gerd Ritter, Lloyd Old, and Carl Batt

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