Apoptosis Methods and Protocols (Methods in Molecular Biology Series, Volume 282)

Apoptosis Methods and Protocols (Methods in Molecular Biology Series, Volume 282)

by Hugh J. M. Brady
     
 

A collection of cutting-edge techniques for detecting and quantifying apoptosis, understanding its biochemistry, and for identifying the genes and proteins that regulate and carry it out. Described in step-by-step detail, these readily reproducible methods range from flow cytometry and immunohistochemical procedures to kinase activity assays, yeast two-hybrid… See more details below

Overview

A collection of cutting-edge techniques for detecting and quantifying apoptosis, understanding its biochemistry, and for identifying the genes and proteins that regulate and carry it out. Described in step-by-step detail, these readily reproducible methods range from flow cytometry and immunohistochemical procedures to kinase activity assays, yeast two-hybrid screening, and the cloning of novel genes by differential expression. The protocols follow the successful Methods in Molecular Biology™ series format, each one offering step-by-step laboratory instructions, an introduction outlining the principle behind the technique, lists of equipment and reagents, and tips on troubleshooting and avoiding known pitfalls. Apoptosis Methods and Protocols constitutes a key technical reference to the significant methodologies used in the field, as well as offering novice and experienced researchers alike powerful tools to illuminate the phenomenon of programmed cell death.

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Product Details

ISBN-13:
9780896038738
Publisher:
Springer-Verlag New York, LLC
Publication date:
01/28/2004
Series:
Methods in Molecular Biology Series, #282
Pages:
320
Product dimensions:
6.34(w) x 9.22(h) x 1.17(d)

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