Apoptosis Methods in Pharmacology and Toxicology: Approaches to Measurement and Quantification

by Myrtle A. Davis (Editor)

Overview

Dr. Myrtle A. Davis has assembled a panel of cutting-edge scientists to describe their best methods for detecting, illuminating, and quantifying apoptotic mechanisms in a way that is useful for the design of toxicology and pharmacology studies. These state-of-the-art techniques include flow cytometric, fluorometric, and laser scanning methods for quantifying and characterizing apoptosis, as well as prools for the use of DNA microarray technology, high throughput screens, and ELISA. Immunocyhemical methods for measuring biochemical and molecular endpoints in tissue sections will be highly useful for those carrying out studies in whole animal models as opposed to cell culture systems.

Product Details

ISBN-13:	9780896038905
Publisher:	Springer-Verlag New York, LLC
Publication date:	06/25/2002
Series:	Methods in Pharmacology and Toxicology
Edition description:	2002
Pages:	156
Product dimensions:	6.10(w) x 9.25(h) x 0.02(d)

Table of Contents

Flow Cytometric and Fluorometric Methods of Quantifying and Characterizing Apoptotic Cell Death.- Analysis of Apoptosis by Laser-Scanning Cytometry.- Specific Methods for Detection and Quantification of Apoptosis in Tissue Sections.- DNA Microarrays.- Microarray Analysis of Apoptosis.- ELISAs for Quantification of Bcl-2 Family Activities and Active Caspases.

Editorial Reviews

Davis (U. of Maryland School of Medicine) presents seven contributions exploring the mechanisms of chemical-induced programmed cell death (apoptosis) in toxicological and pharmacological research. Striving for methods of detection and quantification that are broadly applicable to numerous tissue and cell types, authors discuss cytometric and fluorometric methods, the application of laser scanning cytometry, DNA microarray analysis and measurement, and relatively new ELISA (enzyme-linked immunosorbent assays) techniques. Annotation c. Book News, Inc., Portland, OR (booknews.com)

"The book emphasizes techniques that can be used to quantify apoptosis, such as cell morphology, flow cytometry, laser-scanning cytometry, ELISA, and DNA microarray. The flow cytometry and ELISA sections are the best, as they are applicable to a wide range of laboratories because of their ease of use do not require any extraordinary facilities. . .The book is well written and instructive, and the techniques described can be applied easily to topics in pharmacology and toxicology as well as in general apoptosis research. A strong point is that the authors emphasize the need to combine morphological assessment of the cell with other apoptotic parameters in order to fully characterize a cell as apoptotic."-Doody's Health Science Book Review Journal

From the Publisher