BN.com Gift Guide

Aptamers in Bioanalysis, Bioelectrochemistry and Biotechnology / Edition 1

Hardcover (Print)
Used and New from Other Sellers
Used and New from Other Sellers
from $116.88
Usually ships in 1-2 business days
(Save 25%)
Other sellers (Hardcover)
  • All (5) from $116.88   
  • New (3) from $116.88   
  • Used (2) from $127.85   
Close
Sort by
Page 1 of 1
Showing All
Note: Marketplace items are not eligible for any BN.com coupons and promotions
$116.88
Seller since 2008

Feedback rating:

(17866)

Condition:

New — never opened or used in original packaging.

Like New — packaging may have been opened. A "Like New" item is suitable to give as a gift.

Very Good — may have minor signs of wear on packaging but item works perfectly and has no damage.

Good — item is in good condition but packaging may have signs of shelf wear/aging or torn packaging. All specific defects should be noted in the Comments section associated with each item.

Acceptable — item is in working order but may show signs of wear such as scratches or torn packaging. All specific defects should be noted in the Comments section associated with each item.

Used — An item that has been opened and may show signs of wear. All specific defects should be noted in the Comments section associated with each item.

Refurbished — A used item that has been renewed or updated and verified to be in proper working condition. Not necessarily completed by the original manufacturer.

New
Brand New, Perfect Condition, Please allow 4-14 business days for delivery. 100% Money Back Guarantee, Over 1,000,000 customers served.

Ships from: Westminster, MD

Usually ships in 1-2 business days

  • Canadian
  • International
  • Standard, 48 States
  • Standard (AK, HI)
$116.89
Seller since 2009

Feedback rating:

(128)

Condition: New
New Book from multilingual publisher. Shipped from UK within 4 to 14 business days. Please check language within??the description. Established seller since 2000.

Ships from: Fairford, United Kingdom

Usually ships in 1-2 business days

  • Standard, 48 States
  • Standard (AK, HI)
$121.13
Seller since 2008

Feedback rating:

(4534)

Condition: New
New Book. Shipped from UK within 4 to 14 business days. Established seller since 2000.

Ships from: Horcott Rd, Fairford, United Kingdom

Usually ships in 1-2 business days

  • Standard, 48 States
  • Standard (AK, HI)
Page 1 of 1
Showing All
Close
Sort by

Overview

This is the first book to detail bioanalytical technologies and methods that have been developed using aptamers in analytical, medical, environmental, and food science applications. After an introduction to aptamers, aptamer targets, and their general uses, it discusses different applications with particular attention to the comparison between aptamer-based biosensors and methods versus the corresponding immunosensors. Examples of aptamer-based diagnostic techniques include whole-cell protein profiling (proteomics) and medical diagnostics for the distinction of diseased versus healthy states. This is a core reference for analytical chemists, electrochemists, pharmaceutical/medicinal chemists, biotechnologists, and others.

Read More Show Less

Editorial Reviews

From the Publisher
'This book provides descriptions of many applications of aptamers for biosensing, and for this reason, it is very useful and an interesting read.? (JACS , August 2009)
Read More Show Less

Product Details

  • ISBN-13: 9780470148303
  • Publisher: Wiley
  • Publication date: 12/31/2008
  • Edition description: New Edition
  • Edition number: 1
  • Pages: 325
  • Product dimensions: 6.30 (w) x 9.40 (h) x 2.90 (d)

Meet the Author

MARCO MASCINI, PhD, is a Full Professor of Analytical Chemistry in the Department of Chemistry at the University of Firenze, Italy. He has been one of the pioneers of biosensor technology. His research interests are related to electrochemical, piezoelectric, and optical biosensors. He pioneered several practical applications of these devices in industrial prototypes for use in bioanalysis, medicine, the environment, and food quality and safety. Some of these devices are commercialized today. Dr. Mascini is the author of more than 300 publications and has taken part in several European Commission projects and steering committees.

Read More Show Less

Table of Contents

PREFACE.

CONTRIBUTORS.

I INTRODUCTION.

1 APTAMERS: LIGANDS FOR ALL REASONS (Jean-Jacques Toulme , Jean-Pierre Daguer, and Eric Dausse).

1.1 Introduction.

1.2 The Power of Selection and Aptamer Refinement.

1.3 The Chemistry Drives the Shape.

1.4 Aptaregulators.

1.5 Aptasensors.

1.6 Prospects.

References.

2 SELEX AND ITS RECENT OPTIMIZATIONS (Beate Strehlitz and Regina Stoltenburg).

2.1 Introduction.

2.2 Aptamers and Their Selection by SELEX.

2.3 Modifications of SELEX Technology.

2.4 Advantages and Limitations of Aptamers and Their Selection Technology.

2.5 Applications of Aptamers Being Developed for the Market.

2.6 Future Perspectives.

References.

II BIOSENSORS.

3 ELECTROCHEMICAL APTASENSORS (Itamar Willner and Maya Zayats).

3.1 Introduction.

3.2 Electrochemical Aptasensor Based on Redox-Active Aptamer Monolayers Linked to Electrodes.

3.3 Enzyme-Based Amplified Electrochemical Aptasensors.

3.4 Amplified Electrochemical Aptasensors Based on Nanoparticles.

3.5 Label-Free Electrochemical Aptasensors.

3.6 Field-Effect Transistor–Based Aptasensors.

3.7 Conclusions and Perspectives.

References.

4 APTAMERS: HYBRIDS BETWEEN NATURE AND TECHNOLOGY (Moritz K. Beissenhirtz, Eik Leupold, Walter Stocklein, Ulla Wollenberger, Oliver Panke, Fred Lisdat, and Frieder W. Scheller).

4.1 Introduction.

4.2 Specific Features of Aptamers.

4.3 Electrochemical Detection of Nucleic Acids.

4.4 Cytochrome c Binding by Aptamers.

4.5 DNA Machines and Aptamers.

References.

5 DETECTION OF PROTEIN–APTAMER INTERACTIONS BY MEANS OF ELECTROCHEMICAL INDICATORS AND TRANSVERSE SHEAR MODE METHOD (Tibor Hianik).

5.1 Introduction.

5.2 Immobilization of Aptamers on a Solid Support.

5.3 Detection of Aptamer–Ligand Interactions.

5.3.1 Electrochemical Methods.

5.3.2 Acoustic Methods.

5.4 Conclusions.

References.

6 BIOSENSORS USING THE APTAMERIC ENZYME SUBUNIT: THE USE OF APTAMERS IN THE ALLOSTERIC CONTROL OF ENZYMES (Kazunori Ikebukuro, Wataru Yoshida, and Koji Sode).

6.1 Aptamers as Molecular Recognition Elements of Biosensors.

6.1.1 Comparing Aptamers to Antibodies.

6.1.2 Signaling Aptamers.

6.2 Homogeneous Sensing.

6.2.1 Biosensor Systems That Do Not Require Bound–Free Separation.

6.2.2 Aptameric Enzyme Subunit.

6.3 Evolution-mimicking Algorithm for the Improvement of Aptamers.

References.

7 NANOMATERIAL-BASED LABEL-FREE APTASENSORS (Kagan Kerman and Eiichi Tamiya).

7.1 Introduction.

7.2 Label-Free Electrochemical Aptasensors.

7.3 Field-Effect Transistor–Based Aptasensors.

7.4 Label-Free Aptasensors Based on Localized Surface Plasmon Resonance.

7.5 Forthcoming Challenges and Concluding Remarks.

References.

8 APTAMER-BASED BIOANALYTICAL ASSAYS: AMPLIFICATION STRATEGIES (Sara Tombelli, Maria Minunni, and Marco Mascini).

8.1 Introduction.

8.2 Bioanalytical Assays Based on Aptamer-Functionalized Nanoparticles.

8.3 Aptamers and Quantum Dot–Based Assays.

8.4 Aptazymes and Aptamer-Based Machines.

8.5 Polymerase Chain Reaction as an Amplification Method in Aptamer-Based Assays.

8.6 Conclusions.

References.

III APPLICATIONS.

9 KINETIC CAPILLARY ELECTROPHORESIS FOR SELECTION, CHARACTERIZATION, AND ANALYTICAL UTILIZATION OF APTAMERS (Sergey N. Krylov).

9.1 Introduction.

9.1.1 Kinetic Capillary Electrophoresis.

9.1.2 The Concept of NECEEM and ECEEM.

9.2 Selection of Aptamers Using KCE Methods for Partitioning and Affinity Control.

9.2.1 NECEEM-Based Selection of Aptamers.

9.2.2 ECEEM-Based Selection of Aptamers.

9.2.3 Optimization of PCR.

9.2.4 Future of KCE Methods for Aptamer Selection.

9.3 Measurements of Binding Parameters of Target–Aptamer Interaction by KCE Methods.

9.3.1 Foundation.

9.3.2 Temperature Control Inside the Capillary.

9.3.3 Examples.

9.4 Quantitative Affinity Analysis of a Target Using Aptamer as an Affinity Probe.

9.4.1 Foundation.

9.4.2 Example.

9.5 Conclusions.

References.

10 APTAMERS FOR SEPARATION OF ENANTIOMERS (Corinne Ravelet and Eric Peyrin).

10.1 Introduction.

10.2 Generation and Properties of Enantioselective Aptamers.

10.3 Immobilized Aptamers for Enantiomeric Separation by Liquid Chromatography.

10.3.1 Stationary-Phase Preparation and Column Packing.

10.3.2 DNA Aptamer-Based CSPs.

10.3.3 RNA Aptamer-Based CSPs and the Mirror-Image Strategy.

10.3.4 Class-Specific Aptamer-Based CSPs.

10.4 Aptamers for Analysis of Enantiomers by Capillary Electrophoresis.

10.4.1 Aptamers as Chiral Additives in the Background Electrolyte for CE Enantiomeric Separation.

10.4.2 Aptamers for the Design of an Affinity CE-Based Enantioselective Competitive Assay.

10.5 Conclusions.

References.

11 APTAMER-MODIFIED SURFACES FOR AFFINITY CAPTURE AND DETECTION OF PROTEINS IN CAPILLARY ELECTROPHORESIS AND MALDI–MASS SPECTROMETRY (Linda B. McGown).

11.1 Introduction.

11.2 Aptamer-Modified Capillaries in Affinity Capillary Electrophoresis.

11.3 Aptamer-Modified Surfaces for Affinity MALDI-MS.

11.3.1 Overview.

11.3.2 Affinity MALDI-MS of Thrombin.

11.3.3 Affinity MALDI-MS of IgE.

11.3.4 Summary.

11.4 Beyond Aptamers: Genome-Inspired DNA Binding Ligands.

References.

12 STRATEGY FOR USE OF SMART ROUTES TO PREPARE LABEL-FREE APTASENSORS FOR BIOASSAY USING DIFFERENT TECHNIQUES (Bingling Li, Hui Wei, and Shaojun Dong).

12.1 Introduction.

12.2 Electrochemical Aptasensors.

12.2.1 POSOALF Mode.

12.2.2 PFSOALF Mode.

12.2.3 Electrochemical Impedimetric Aptasensors.

12.2.4 Electrochemical Aptasensors with Nonlabeled Redox Probes.

12.3 Fluorescent Molecular Switches.

12.3.1 POSFALF Mode.

12.3.2 PFSFALF Mode.

12.4 Colorimetry.

12.4.1 POSFALF Mode.

12.4.2 PFSFALF Mode.

12.5 Hemin–Aptamer DNAzyme-Based Aptasensor.

12.6 Liquid Chromatography, Electrochromatography, and Capillary Electrophoresis Applications.

12.7 Other Aptasensors.

12.8 Conclusions.

References.

INDEX.

Read More Show Less

Customer Reviews

Be the first to write a review
( 0 )
Rating Distribution

5 Star

(0)

4 Star

(0)

3 Star

(0)

2 Star

(0)

1 Star

(0)

Your Rating:

Your Name: Create a Pen Name or

Barnes & Noble.com Review Rules

Our reader reviews allow you to share your comments on titles you liked, or didn't, with others. By submitting an online review, you are representing to Barnes & Noble.com that all information contained in your review is original and accurate in all respects, and that the submission of such content by you and the posting of such content by Barnes & Noble.com does not and will not violate the rights of any third party. Please follow the rules below to help ensure that your review can be posted.

Reviews by Our Customers Under the Age of 13

We highly value and respect everyone's opinion concerning the titles we offer. However, we cannot allow persons under the age of 13 to have accounts at BN.com or to post customer reviews. Please see our Terms of Use for more details.

What to exclude from your review:

Please do not write about reviews, commentary, or information posted on the product page. If you see any errors in the information on the product page, please send us an email.

Reviews should not contain any of the following:

  • - HTML tags, profanity, obscenities, vulgarities, or comments that defame anyone
  • - Time-sensitive information such as tour dates, signings, lectures, etc.
  • - Single-word reviews. Other people will read your review to discover why you liked or didn't like the title. Be descriptive.
  • - Comments focusing on the author or that may ruin the ending for others
  • - Phone numbers, addresses, URLs
  • - Pricing and availability information or alternative ordering information
  • - Advertisements or commercial solicitation

Reminder:

  • - By submitting a review, you grant to Barnes & Noble.com and its sublicensees the royalty-free, perpetual, irrevocable right and license to use the review in accordance with the Barnes & Noble.com Terms of Use.
  • - Barnes & Noble.com reserves the right not to post any review -- particularly those that do not follow the terms and conditions of these Rules. Barnes & Noble.com also reserves the right to remove any review at any time without notice.
  • - See Terms of Use for other conditions and disclaimers.
Search for Products You'd Like to Recommend

Recommend other products that relate to your review. Just search for them below and share!

Create a Pen Name

Your Pen Name is your unique identity on BN.com. It will appear on the reviews you write and other website activities. Your Pen Name cannot be edited, changed or deleted once submitted.

 
Your Pen Name can be any combination of alphanumeric characters (plus - and _), and must be at least two characters long.

Continue Anonymously

    If you find inappropriate content, please report it to Barnes & Noble
    Why is this product inappropriate?
    Comments (optional)