Arachidonate Related Lipid Mediators: Volume 187: Arachidonate Related Lipid Mediators


This volume includes information on arachidonic acid metabolites and platelet activating factor. Assays for the quantitative measurement of prostaglandins, leukotrienes, and platelet-activating factor, including enzyme immunoassays and mass spectrophotometric techniques, are presented. Preparation and analysis of cytochrome P-450 metabolites of arachidonic acid are discussed. The isolation, purification, and assay of enzymes important in the biosynthesis and metabolism of these ...

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This volume includes information on arachidonic acid metabolites and platelet activating factor. Assays for the quantitative measurement of prostaglandins, leukotrienes, and platelet-activating factor, including enzyme immunoassays and mass spectrophotometric techniques, are presented. Preparation and analysis of cytochrome P-450 metabolites of arachidonic acid are discussed. The isolation, purification, and assay of enzymes important in the biosynthesis and metabolism of these lipids are included.

Audience: Biochemists, pharmacologists, immunologists, hematologists, endocrinologists, physiologists, oncologists, and other biomedical researchers.

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Editorial Reviews

From the Publisher
Praise for the Volume
"This volume of Methods in Enzymology brings up-to-date the new techniques in the measurement of eicosanoids, the application of molecular biological techniques to eicosanoids, the application of molecular biological techniques to eicosanoid research, and the developments in the study of the action and formation of eicosanoids in cell and tissue systems. The authors are to be commended for bringing together such a truly outstanding compendium, with top-rate articles by most of the leading scientists in the field. The book provides detailed information about individual aspects of eicosanoid research while, at the same time, encompassing the great breadth of the area. It is recommended for all individuals with an interest with eicosanoids and lipid mediators, from graduate students to eminent scientists.
"Seemingly immortal series... the chapters are well written and the protocols...can be followed without further reference. This is only to be expected from a standard methods text."
Praise for the Series
"The Methods in Enzymology series represents the gold-standard."
"Incomparably useful."
"It is a true 'methods' series, including almost every detail from basic theory to sources of equipment and reagents, with timely documentation provided on each page."
"The series has been following the growing, changing and creation of new areas of science. It should be on the shelves of all libraries in the world as a whole collection."
"The appearance of another volume in that excellent series, Methods in Enzymology, is always a cause for appreciation for those who wish to successfully carry out a particular technique or prepare an enzyme or metabolic intermediate without the tiresome prospect of searching through unfamiliar literature and perhaps selecting an unproven method which is not easily reproduced."
"If we had some way to find the work most often consulted in the laboratory, it could well be the multi-volume series Methods in Enzymology...a great work."
"A series that has established itself as a definitive reference for biochemists."
Supplements the earlier Prostaglandins and arachidonate metabolites (v.86 of the series) with the addition of new techniques in the measurement of eicosanoids, of new molecular biological techniques applied to eicosanoid research, and of descriptions of methods used to study the action and formation of eicosanoids in isolated cell and tissue systems. Annotation c. Book News, Inc., Portland, OR (
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Product Details

Meet the Author

Table of Contents

W.L. Smith, P. Borgeat, M. Hamberg, L.J. Roberts II, A.L. Willis, S. Yamamoto, P.W. Ramwell, J. Rokach, B. Samuelsson, E.J. Corey, and C.R. Pace-Asciak, Nomenclature.
Assays: Prostaglandins:
I.A. Blair, Electron-Capture Negative-Ion Chemical Ionization Mass Spectrometry of Lipid Mediators.
P. Pradelles, J. Grassi, and J. Maclouf, Enzyme Immunoassays of Eicosanoids Using Acetylcholinesterase.
G. Ciabattoni, P. Patrignani, and C. Patrono, Radioimmunoassay of 11-Dehydrothromboxane B2.
F. Catella and G.A. FitzGerald, Measurement of Thromboxane Metabolites by Gas Chromatography-Mass Spectrometry.
D.F. Wendelborn, J.D. Morrow, and L.J. Roberts II, Quantification of Prostaglandin F2 Stable Isotope Dilution Mass Spectrometric Assay.
J.J. Vrbanac, J.W. Cox, T.D. Eller, and D.R. Knapp, Immunoaffinity Purification-Chromatographic Quantitative Analysis of Arachidonic Acid Metabolites.
J.-P. Lellouche, J.P. Beaucourt, and A. Vanhove, Preparation of Tetradeuterated Leukotriene A4 Methyl Ester: Methyl-[11,12,14,15-2H4]-(5S,6S)-Oxide-(7E,9E,11Z,14Z)-Eicosatetraenoate.
W.R. Mathews, Quantitative Gas Chromatography-Mass Spectrometry Analysis of Leukotriene B4.
P. Pradelles, C. Antoine, J.-P. Lellouche, and J. Maclouf, Enzyme Immunoassays for Leukotrienes C4 and E4 Using Acetylcholinesterase.
R.C. Murphy and A. Sala, Quantitation of Sulfidopeptide Leukotrienes in Biological Fluids by Gas Chromatography-Mass Spectrometry.
P. Borgeat, S. Picard, P. Vallerand, S. Bourgoin, A. Odeimat, P. Sirois, and P.E. Poubelle, Automated On-Line Extraction and Profiling of Lipoxygenase Products of Arachidonic Acid by High-Performance Liquid Chromatography.
E.C. Hayes, Preparation of Antibodies Directed against Leukotrienes.
Platelet-Activating Factor:
M.J. Bossant, E. Ninio, D. Delautier, and J. Benveniste, Bioassay of Platelet-Activating Factor Acether by Rabbit Platelet Aggregation.
P.M. Henson, Bioassay of Platelet-Activating Factor by Release of [3H]Serotonin.
K.L. Clay, Quantitation of Platelet-Activating Factor by Gas Chromatography-Mass Spectrometry.
W.C. Pickett and C.S. Ramesha, Quantitative Analysis of Platelet-Activating Factor by Gas Chromatography-Negative-Ion Chemical Ionization Mass Spectrometry.
D.J. Hanahan, Isolation of Platelet-Activating Factor and Purification by Thin-Layer Chromatography.
F.H. Chilton, Separation and Characterization of Arachidonate-Containing Phosphoglycerides.
Other Oxidative Products:
C.N. Serhan, High-Performance Liquid Chromatography Separation and Determination of Lipoxins.
J. Turk, W.T. Stump, W. Conrad-Kessel, R. Seabold, and B. Wolf, Quantitation of Epoxy- and Dihydroxyeicosatrienoic Acids by Stable Isotope-Dilution Mass Spectrometry.
A.R. Brash and D.J. Hawkins, High-Performance Liquid Chromatography for Chiral Analysis of Eicosanoids.
Phospholipase A2 and Phospholipids:
G.M. Patton and S.J. Robins, Extraction of Phospholipids and Analysis of Phospholipid Molecular Species.
C.C. Leslie, Macrophage Phospholipase A2 Specific for sn-2-Arachidonic Acid.
J.E. Bleasdale, J.C. McGuire, and G.A. Bala, Measurement of Phosphoinositide-Specific Phospholipase C Activity.
M. Laposata, Solubilization of Arachidonate CoA Ligase from Cell Membranes, Chromatographic Separation from Nonspecific Long-Chain-Fatty-Acid-CoA Ligase, and Isolation of Mutant Cell Line Defective in Arachidonate CoA Ligase.
Biosynthesis, Enzymology, and Chemical Synthesis: Prostaglandins:
D. Venton, G. Le Breton, and E. Hall, Preparation of Prostaglandin H2: Extended Purification/Analysis Scheme.
A.S. Muerhoff, D.E. Williams, and B.S. Masters, Purification and Properties of Pregnancy-Inducible Rabbit Lung Cytochrome P-450 Prostaglandin w-Hydroxylase. Leukotrienes.
P. Reddanna, J. Whelan, K.R. Maddipati, and C.C. Reddy, Purification of Arachidonate 5-Lipoxygenase from Potato Tubers.
M.A. Shirley and D.O. Stene, Leukotriene Metabolism by Isolated Rat Hepatocytes.
N. Ohishi, T. Izumi, Y. Seyama, and T. Shimizu, Purification and Characterization of Human Lung Leukotriene A4 Hydrolase.
T. Shimizu, Z.-i. Honda, I. Miki, Y. Seyama, T. Izumi, O. Radmark, and B. Samuelsson, Potato Arachidonate 5-Lipoxygenase: Purification, Characterization, and Preparation of 5(S)-Hydroperoxyeicosatetraenoic Acid.
M. Söderstr*adom, B. Mannervik, and S. Hammarström, Leukotriene C-Synthase: Characterization in Mouse Mastocytoma Cells.
C.A. Rouzer and B. Samuelsson, Leukocyte Arachidonate 5-Lipoxygenase: Isolation and Characterization.
R.J. Soberman, Cytochrome P-450 LTB and Inactivation of Leukotriene B4.
J.Z. Haeggström, Cytosolic Liver Enzymes Catalyzing Hydrolysis of Leukotriene A4 Leukotriene B4 and 5,6-Dihydroxyeicosatetraenoic Acid.
R.J. Soberman, Purification and Properties of Leukotriene C4 Synthase from Guinea Pig Lung Microsomes.
N. Ueda and S. Yamamoto, Immunoaffinity Purification of Arachidonate 5-Lipoxygenase from Porcine Leukocytes.
Platelet-Activating Factor:
D.M. Stafforini, T.M. McIntyre, and S.M. Prescott, Platelet-Activating Factor Acetylhydrolase from Human Plasma.
Other Oxidative Products:
J.R. Falck, P. Yadagiri, and J. Capdevila, Synthesis of Epoxyeicosatrienoic Acids and Heteroatom Analogs.
M.A. Carroll, E.D. Drugge, C.E. Dunn, and J.C. McGiff, Isolation of Rabbit Renomedullary Cells and Arachidonate Metabolism.
M.L. Schwartzman and N.G. Abraham, Ocular Cytochrome P-450 Metabolism of Arachidonate: Synthesis and Bioassay.
J.H. Capdevila, J.R. Falck, E. Dishman, and A. Karara, Cytochrome P-450 Arachidonate Oxygenase.
Pharmacology: Antagonist and Synthesis Inhibitors: Prostaglandins:
P.V. Haluska, T.A. Morinelli, and D.E. Mais, Radioligand Binding Assays for Thromboxane A2/Prostaglandin H2 Receptors.
G.C. Le Breton, C.T. Lim, C.M. Vaidya, and D.L. Venton, Thromboxane A2/Prostaglandin H2 Receptor Antagonists.
D. Aharony, Assessment of Leukotriene D4 Receptor Antagonists.
S. Mong, Sulfidopeptide Leukotriene Receptor Binding Assays.
Platelet-Activating Factor:
D.J. Hosford, M.T. Domingo, P.E. Chabrier, and P. Braquet, Ginkgolides and Platelet-Activating Factor Binding Sites.
T.Y. Shen and I.M. Hussaini, Kadsurenone and Other Related Ligdans as Antagonists of Platelet-Activating Factor Receptor.
J. Casals-Stenzel and H.O. Heuer, Use of WEB 2086 and WEB 2170 as Platelet-Activating Factor Antagonists.
Molecular Biology: Prostaglandins:
D.L. DeWitt and W.L. Smith, Cloning of Sheep and Mouse Prostaglandin Endoperoxide Synthases.
R. Odenwaller, Y.-N.P. Chen, and L.J. Marnett, Preparation and Proteolytic Cleavage of Apoprostaglandin Endoperopide Synthase.
O. Rädmark, C. Funk, J.Y. Fu, T. Matsumoto, H. Jörnvall, B. Samuelsson, M. Minami, S. Ohno, H. Kawasaki, Y. Seyama, K. Suzuki, and T. Shimizu, Cloning of Leukotriene A4 Hydrolase cDNA.
C.D. Funk, T. Matsumoto, and B. Samuelsson, Molecular Biology and Cloning of Arachidonate 5-Lipoxygenase.
Cell Models of Lipid Mediator Production: Isolated Cell Preparations:
N.A. Payne and J.G. Gerber, Parietal Cell Preparation and Arachidonate Metabolism.
E. Razin, Culture of Bone Marrow-Derived Mast Cells: A Model for Studying Oxidative Metabolism of Arachidonic Acid and Synthesis of Other Molecules Derived from Membrane.
G.A. Zimmerman, R.E. Whatley, T.M. McIntyre, D.M. Benson, and S.M. Prescott, Endothelial Cells for Studies of Platelet-Activating Factor and Arachidonate Metabolites.
R.G. Gorman, M.J. Bienkowski, and C.W. Benjamin, Use of Cultured Cells to Study Arachidonic Acid Metabolism.
M.S. Simonson and M.J. Dunn, Eicosanoid Biochemistry in Cultured Glomerular Mesangial Cells.
Cell-Cell Interactions:
D.A. Jones and F.A. Fitzpatrick, Leukotriene B4 Biosynthesis by Erythrocyte-Neutrophil Interactions.
S.J. Feinmark, Leukotriene C4 Biosynthesis during Polymorphonuclear Leukocyte-Vascular Cell Interactions.
C.A. Dahinden and U. Wirthmüller, Release and Metabolism of Leukotriene in Neutrophil-Mast Cell Interactions.
K.K. Wu and A.C. Papp, Interaction between Platelets and Lymphocytes in Biosynthesis of Prostacyclin.
A.J. Marcus, Eicosanoid Interactions between Platelets, Endothelial Cells, and Neutrophils.
In Vitro Tissue Preparations:
S.-W. Chang and N.F. Voelkel, Isolated Perfused Rat Lung in Arachidonate Studies.
R. Levi and K.M. Mullane, Isolated Coronary-Perfused Mammalian Heart: Assessment of Eicosanoid and Platelet-Activating Factor Release and Effects.
T. Vigano, M.T. Crivellari, M. Mezzetti, and G.C. Folco, Preparation of Human and Animal Lung Tissue for Eicosanoid Research.
Each chapter includes references.
Author Index.
Subject Index.

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