Avidin-Biotin Interactions: Methods and Applications / Edition 1

Hardcover (Print)
Buy New
Buy New from BN.com
$90.38
Used and New from Other Sellers
Used and New from Other Sellers
from $3.03
Usually ships in 1-2 business days
(Save 97%)
Other sellers (Hardcover)
  • All (9) from $3.03   
  • New (6) from $14.92   
  • Used (3) from $3.03   

Overview

For researchers today, the avidin-biotin interaction is among the most widely exploited among biomedical scientists. Despite its popularity, avidin-biotin methodology suffers from a lack of clarity associated with popular, low cost, prefabricated kits used by many in the field. In Avidin-Biotin Interactions: Methods and Applications, leaders in avidin-biotin methodology share their knowledge relating to the application of the extremely powerful interaction between the protein avidin or its homologues and the vitamin biotin and some of its homologues. Although avidin-biotin based kits have been produced for quite some time and remain a widely used methodology, many of the chapters in this text describe situations in which the previous methodology failed due to some factor, and the authors developed new and better ways for the avidin-biotin system to be utilized. With excellent descriptions of laboratory protocols written by expert researchers, this volume is equally perfect for the student or the professional laboratory scientist. Each chapter in Avidin-Biotin Interactions: Methods and Applications contains not only excellent descriptions of protocols for the lab, but also the experience of researchers who used specific methodology, found a problem with that methodology in a new setting, and ultimately devised ways to minimize or obviate the limitations of the technology.
Read More Show Less

Editorial Reviews

From the Publisher
Aus den Rezensionen:

“... Das ... editierte Methodenbuch gliedert sich in siebzehn Beiträge, die von auf dem jeweiligen Gebiet ausgewiesenen Autoren verfasst wurden. ... Die angegebenen Protokolle ermöglichen, einem Methodenbuch entsprechend, eine zeitsparende und kostengünstige Präparation ... Positiv ist auch die gute Darstellung der Faktoren, die für Biotinylierungsreaktionen ... kritisch sind. ... Das Buch ist nach Form und Inhalt in jedem Fall geeignet, dem Leser die weitere Vertiefung in die aktuelle Literatur zu ermöglichen und Hinweise für Applikationen zu geben. ... eine umfassende Informationsquelle und Protokolle zur Verfügung ...“ (Dr. rer. nat. H. Walzel, in: Laboratoriums-Medizin: journal of laboratory medicine, 2009, Vol.33, Issue 2, S. 129 f.)

Read More Show Less

Product Details

  • ISBN-13: 9781588295835
  • Publisher: Springer-Verlag New York, LLC
  • Publication date: 2/26/2008
  • Series: Methods in Molecular Biology Series , #418
  • Edition description: 2008
  • Edition number: 1
  • Pages: 232
  • Product dimensions: 6.00 (w) x 9.10 (h) x 0.70 (d)

Table of Contents

Table of Contents

Preface………………………………………………………………………...Robert J. McMahon Preparation of avidin conjugates……………………… Rosaria Haugland and Mahesh Bhalgat Coupling of antibodies with biotin…………………………...Rosaria Haugland and Wendy You Optimization of detection and quantification of proteins on membranes in very high and very low abundance using avidin and streptavidin…………………….Sara R. Zwart and Brandon J. Lewis Use of the Avidin (Imino)biotin System as a general approach to affinity precipitation………………………………………………………..Ruth Freitag and Frank Hilbrig Capturing biotinylated proteins and peptides by avidin functional affinity electrophoresis.........................................................................................Bao-Shiang Lee, Sangeeth Krishnanchettiar, Syed Salman Lateef, and Shalini Gupta Functionality Screening of Streptavidin Mutants by Non-Denaturing SDS-PAGE Using Biotin-4-Fluorescein………………………………………………..Nicolas Humbert and Thomas R. Ward Application of biotin-4-fluorescein in homogeneous fluorescence assays for avidin, streptavidin, and biotin or biotin derivatives…………………………………………..Andreas Ebner, Markus Marek, Karl Kaiser, Gerald Kada, Christoph D. Hahn, Bernd Lackner, and Hermann J. Gruber Quantitative recovery of biotinylated proteins from streptavidin-based affinity chromatography resins…………………………..Christoph Rosli, Jascha-N. Rybak, Dario Neri, and Giuliano Elia High yield production and purification of recombinant T7-tag-mature-streptavidin in glucose stressed E. coli….Nicolas Humbert, Peter Schurmann, Andrea Zocchi, Jean-Marc Neuhaus and Thomas R. Ward Endogenous biotin in rat brain: implications for false-positive results with avidin- and streptavidin-biotin techniques..Bruce E. McKay, Michael L. Molineux, and Ray W. Turner Pseudo-Immunolabelling with the Avidin-Biotin-Peroxidase Complex (ABC) due to the presence of Endogenous Biotin in the Retina…..Willem Kamphuis and Jan Klooster Use of synthetic peptides for identifying biotinylation sites in human histones…………Gabriela Camporeale, Yap Ching Chew, Alice Keuh, Gautam Sarath and Janos Zempleni Detection and quantitation of DNA methylation using a biotin/avidin microplate assay…Kirsten Liebert and Albert Jeltsch Anti-biotin antibodies offer superior organelle-specific labeling of mihondria over avidin or streptavidin……………………………Elisabeth D. Coene, Michael K. Shaw, and David J. Vaux Mammalian lectin as a tool in glycol- and hishemistry with relevance for diagnostic procedure……………………………………………………Karel Smetana, Jr. and Sabine Andre A multiplex approach to isotyping antigen-specific antibodies using biotinylated antigen/streptavidin-phycoerythrin…………………………… Anna Bogusiewicz

Read More Show Less

Customer Reviews

Be the first to write a review
( 0 )
Rating Distribution

5 Star

(0)

4 Star

(0)

3 Star

(0)

2 Star

(0)

1 Star

(0)

Your Rating:

Your Name: Create a Pen Name or

Barnes & Noble.com Review Rules

Our reader reviews allow you to share your comments on titles you liked, or didn't, with others. By submitting an online review, you are representing to Barnes & Noble.com that all information contained in your review is original and accurate in all respects, and that the submission of such content by you and the posting of such content by Barnes & Noble.com does not and will not violate the rights of any third party. Please follow the rules below to help ensure that your review can be posted.

Reviews by Our Customers Under the Age of 13

We highly value and respect everyone's opinion concerning the titles we offer. However, we cannot allow persons under the age of 13 to have accounts at BN.com or to post customer reviews. Please see our Terms of Use for more details.

What to exclude from your review:

Please do not write about reviews, commentary, or information posted on the product page. If you see any errors in the information on the product page, please send us an email.

Reviews should not contain any of the following:

  • - HTML tags, profanity, obscenities, vulgarities, or comments that defame anyone
  • - Time-sensitive information such as tour dates, signings, lectures, etc.
  • - Single-word reviews. Other people will read your review to discover why you liked or didn't like the title. Be descriptive.
  • - Comments focusing on the author or that may ruin the ending for others
  • - Phone numbers, addresses, URLs
  • - Pricing and availability information or alternative ordering information
  • - Advertisements or commercial solicitation

Reminder:

  • - By submitting a review, you grant to Barnes & Noble.com and its sublicensees the royalty-free, perpetual, irrevocable right and license to use the review in accordance with the Barnes & Noble.com Terms of Use.
  • - Barnes & Noble.com reserves the right not to post any review -- particularly those that do not follow the terms and conditions of these Rules. Barnes & Noble.com also reserves the right to remove any review at any time without notice.
  • - See Terms of Use for other conditions and disclaimers.
Search for Products You'd Like to Recommend

Recommend other products that relate to your review. Just search for them below and share!

Create a Pen Name

Your Pen Name is your unique identity on BN.com. It will appear on the reviews you write and other website activities. Your Pen Name cannot be edited, changed or deleted once submitted.

 
Your Pen Name can be any combination of alphanumeric characters (plus - and _), and must be at least two characters long.

Continue Anonymously

    If you find inappropriate content, please report it to Barnes & Noble
    Why is this product inappropriate?
    Comments (optional)