Bacterial Pathogenesis: Methods and Protocols / Edition 1

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Bacterial infections affect world health today as a leading cause of morbidity and mortality. Pathogenic bacteria routinely command a broad spectrum of niches in the human host, making an understanding of pathogenesis mechanisms crucial to the development of prophylactics and treatment for bacterial diseases. A variety of in vitro methods, in vivo animal model systems and cutting-edge genomics assays have arisen in the effort to study bacterial pathogenesis and identify potential therapeutic targets. In Bacterial Pathogenesis, in-depth methods and state-of-the-art protocols are presented for investigating specific mechanisms of pathogenesis for a wide range of bacteria. This invaluable collection includes protocols to study host-pathogen interactions, animal models of infection, and novel approaches to identifying therapeutic targets designed to control infections. Up-to-date molecular typing methods for Staphylococcus aureus and a new model of streptococcal pharyngitis in non-human primates are also included. Bacterial Pathogenesis will prove an invaluable collection for microbiologists, immunologists, cell biologists and infectious disease clinicians - and indispensable to all science researchers interested in studying pathogenic bacteria and related disease processes. Written for: Microbiologists, immunologists, cell biologists, infectious disease clinicians, pathogenic bacteria researchers

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Editorial Reviews

From the Publisher
From the reviews:

"An eclectic collection of papers ranges from those with limited general applications, to those with a much broader interest. … The additional notes in each paper are most useful, and may help those making use of these techniques avoid some of the frustrations associated with following methodologies in published journal papers. … a volume to dip into seeking inspiration for new techniques to apply to one’s own favourite organism." (Jonathan Fletcher, Microbiology Today, July, 2008)

"This book was written to bring scientists working in the field of bacterial pathogenesis up-to-date with both traditional tried-and-tested methods that have been in use for decades, and more recent technological advances and breakthroughs that have led to the development of new prools. … On the whole this book is very informative and includes an extensive set of complex and detailed prools. … I would recommend to anyone in the field or thinking of entering the field of bacterial pathogenesis." (Andrew Vaughan, Immunology News, August, 2008)

"The 431st volume of Methods in Molecular Biology published by Humana Press is dedicated to the problems of bacterial pathogenesis. ... Each chapter is concluded by an exhaustive list of references. ... By its high level of interest and schematic approach the book is suitable not only for scientists dealing with bacterial pathogenesis ... but it can be recommended also for graduate students in microbiology who are about to start their scientific career." (K. Tyllovâ, Folia Microbiologica, Vol. 53, November, 2008)

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Product Details

  • ISBN-13: 9781588297402
  • Publisher: Springer-Verlag New York, LLC
  • Publication date: 1/28/2008
  • Series: Methods in Molecular Biology Series, #431
  • Edition description: 2008
  • Edition number: 1
  • Pages: 320
  • Product dimensions: 6.30 (w) x 9.30 (h) x 1.00 (d)

Table of Contents

Part I. Mechanisms of Bacterial Pathogenesis

1 Isolation and Characterization of Lipopolysaccharides

Michael A. Apicella

2 Proteomic Analysis of Proteins Secreted by Strepoccus pyogenes

Michelle A. Chaussee, Emily J. McDowell, and Michael S. Chaussee

3 Proteomic Analysis to Investigate Regulatory Networks in Staphylococcus aureus

Susanne Engelmann and Michael Hecker

4 Microarray Comparative Genomic Hybridization for the Analysis of Bacterial Population Genetics and Evolution

Caitriona M. Guinane and J. Ross Fitzgerald

5 Detection and Inhibition of Bacterial Cell-Cell Communication

Scott A. Rice, Diane McDougald, Michael Givskov, and Staffan Kjelleberg

6 A System for Site-Specific Genetic Manipulation of the Relapsing Fever Spirochete Borrelia hermsii

James M. Battisti, Sandra J. Raffel, and Tom G. Schwan

7 Transposon Mutagenesis of the Lyme Disease Agent Borrelia burgdorferi

Philip E. Stewart and Patricia A. Rosa

8 The Biofilm Exopolysaccharide Polysaccharide Intercellular Adhesin (PIA) – A Molecular and Biochemical Approach

Cuong Vuong and Michael Otto

Part II. Host-Pathogen Interaction

9 Analysis of Staphylococcus aureus Gene Expression during PMN Phagocytosis

Jovanka M. Voyich, Dan E. Sturdevant, and Frank R. DeLeo

10 Examining the Vector-Host-Pathogen Interface with Quantitative Molecular Tools

Jason E. Comer, Ellen A. Lorange, and B. Joseph Hinnebusch

11 Intracellular Localization of Brucella abortus and Francisella tularensis in Primary Murine Macrophages

Jean Celli

12 Rate and Extent of Helicobacter pylori Phagocytosis

Lee-Ann H. Allen

13 Culture, Isolation and Labeling of Anaplasma Phagocytophilum for Subsequent Infection of Human Neutrophils

Dori L. Borjesson

14 Ultrastructural Analysis of Bacteria-Host Cell Interactions

David W. Dorward

15 Infection of Human Monocyte-derived Macrophages with Coxiella burnetii

Jeffrey G. Shannon and Robert A. Heinzen

16 Infection of Epithelial Cells with Salmonella enterica

Olivia Steele-Mortimer

17 Determining the Cellular Targets of ROS in Borrelia burgdorferi

Julie A. Boylan and Frank C. Gherardini

Part III. Animal Models of Bacterial Infection

18 Bioluminescent Imaging of Bacterial Biofilm Infections in vivo

Jagath L. Kadurugamuwa and Kevin P. Francis

19 The Cotton Rat as a Model for Staphylococcus aureus Nasal Colonization in Humans (Cotton Rat S. aureus Nasal Colonization Model)

John Kokai-Kun

20 A Non-Human Primate Model of Acute Group A Strepoccus Pharyngitis

Paul Sumby, Anne H. Tart, and James M. Musser

Part IV. Identification of Therapeutic Targets and Typing Methods

21 Target-based Antimicrobial Drug Discovery

Lefa E. Alksne and Paul M. Dunman

22 Sequence Analysis of the Variable Number Tandem Repeat in Staphylococcus aureus Protein A Gene: spa Typing

Barun Mathema, Jose Mediavilla, and Barry N. Kreiswirth

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