Bacterial Pathogenesis: Methods and Protocols / Edition 1

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Bacterial infections affect world health today as a leading cause of morbidity and mortality. This book presents in-depth methods and state-of-the-art protocols for investigating specific mechanisms of pathogenesis for a wide range of bacteria. Written by experts in the field, this invaluable collection includes protocols to study host-pathogen interactions, animal models of infection, and novel approaches to identifying therapeutic targets designed to control infections.

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Product Details

  • ISBN-13: 9781617377518
  • Publisher: Springer-Verlag New York, LLC
  • Publication date: 12/9/2010
  • Series: Methods in Molecular Biology Series, #431
  • Edition description: Softcover reprint of hardcover 1st ed. 2008
  • Edition number: 1
  • Pages: 320
  • Product dimensions: 0.69 (w) x 9.21 (h) x 6.14 (d)

Table of Contents

Part I. Mechanisms of Bacterial Pathogenesis

1 Isolation and Characterization of Lipopolysaccharides

Michael A. Apicella

2 Proteomic Analysis of Proteins Secreted by Streptococcus pyogenes

Michelle A. Chaussee, Emily J. McDowell, and Michael S. Chaussee

3 Proteomic Analysis to Investigate Regulatory Networks in Staphylococcus aureus

Susanne Engelmann and Michael Hecker

4 Microarray Comparative Genomic Hybridization for the Analysis of Bacterial Population Genetics and Evolution

Caitriona M. Guinane and J. Ross Fitzgerald

5 Detection and Inhibition of Bacterial Cell-Cell Communication

Scott A. Rice, Diane McDougald, Michael Givskov, and Staffan Kjelleberg

6 A System for Site-Specific Genetic Manipulation of the Relapsing Fever Spirochete Borrelia hermsii

James M. Battisti, Sandra J. Raffel, and Tom G. Schwan

7 Transposon Mutagenesis of the Lyme Disease Agent Borrelia burgdorferi

Philip E. Stewart and Patricia A. Rosa

8 The Biofilm Exopolysaccharide Polysaccharide Intercellular Adhesin (PIA) – A Molecular and Biochemical Approach

Cuong Vuong and Michael Otto

Part II. Host-Pathogen Interaction

9 Analysis of Staphylococcus aureus Gene Expression during PMN Phagocytosis

Jovanka M. Voyich, Dan E. Sturdevant, and Frank R. DeLeo

10 Examining the Vector-Host-Pathogen Interface with Quantitative Molecular Tools

Jason E. Comer, Ellen A. Lorange, and B. Joseph Hinnebusch

11 Intracellular Localization of Brucella abortus and Francisella tularensis in Primary Murine Macrophages

Jean Celli

12 Rate and Extent of Helicobacter pylori Phagocytosis

Lee-Ann H. Allen

13 Culture, Isolation and Labeling of Anaplasma Phagocytophilum for Subsequent Infection of Human Neutrophils

Dori L. Borjesson

14 Ultrastructural Analysis of Bacteria-Host Cell Interactions

David W. Dorward

15 Infection of Human Monocyte-derived Macrophages with Coxiella burnetii

Jeffrey G. Shannon and Robert A. Heinzen

16 Infection of Epithelial Cells with Salmonella enterica

Olivia Steele-Mortimer

17 Determining the Cellular Targets of ROS in Borrelia burgdorferi

Julie A. Boylan and Frank C. Gherardini

Part III. Animal Models of Bacterial Infection

18 Bioluminescent Imaging of Bacterial Biofilm Infections in vivo

Jagath L. Kadurugamuwa and Kevin P. Francis

19 The Cotton Rat as a Model for Staphylococcus aureus Nasal Colonization in Humans (Cotton Rat S. aureus Nasal Colonization Model)

John Kokai-Kun

20 A Non-Human Primate Model of Acute Group A Streptococcus Pharyngitis

Paul Sumby, Anne H. Tart, and James M. Musser

Part IV. Identification of Therapeutic Targets and Typing Methods

21 Target-based Antimicrobial Drug Discovery

Lefa E. Alksne and Paul M. Dunman

22 Sequence Analysis of the Variable Number Tandem Repeat in Staphylococcus aureus Protein A Gene: spa Typing

Barun Mathema, Jose Mediavilla, and Barry N. Kreiswirth

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