Basic and Clinical Applications of Flow Cytometry: Proceeding of the 24th Annual Detroit Cancer Symposium Detroit, Michigan, USA - April 30, May 1 and 2, 1992 / Edition 1

by Frederick A. Valeriote, Alexander Nakeff, Manuel Valdivieso

Overview

The focus of this symposium was on the present and future capabilities of flow cytometry for both medical and biological applications in cancer. This technology began with quite modest instrumentation, with limited capabilities to answer biological questions. Today, both the clinical workhorses and the powerful multi-laser, multi-detector, sorting machinery, coupled with sophisticated computers and storage devices and the increasing storehouse of markers and dyes, are taking us to the limit and beyond in finding answers to the cause and cure of cancer.
In the past, both normal hematopoietic tissue and leukemias have been the tissue samples of choice in the application of flow cytometry, and some of the most recent applications with these tissues are presented here. However, the book also discusses the increasingly sophisticated disaggregation techniques which allow investigators the possibility to train their lasers on solid tumors. Not only can we use flow cytometry with associated fluorescent markers to understand the biology of cancer, but also the wide array of existing and developing markers provides us with important diagnostic tools in the detection of cancer early in either the malignant or relapse process. And the field comes full circle, with the use of the technology for gene mapping and other genetic studies to unlock the basic malignant process.

Product Details

ISBN-13:	9780792338093
Publisher:	Springer US
Publication date:	12/31/1995
Series:	Developments in Oncology , #77
Edition description:	1996
Pages:	318
Product dimensions:	6.10(w) x 9.25(h) x 0.03(d)

Table of Contents

1. Cytometry 2000: Laser Probing the Future.- 2. Activation and Proliferation of Purified Hemato-Poietic Precursor Cells from Human Bone Marrow.- 3. Flow Cytometric Proliferative Fraction Analysis in Solid Tumors.- 4. Mitogenic and Non-Mitogenic Induction of Lympho-Cytic Invasion: Dual Parameter flow Cytometric Analysis.- 5. Flow Cytometric Monitoring of Drug Resistance in Human Solid Tumors.- 6. Flow Cytometry in Oncology: Some Lessons from History.- 7. DNA Flow Cytometry Application to Clinical Trials in Breast Cancer.- 8. Processing of Solid Tumors for DNA analysis by Flow Cytometry.- 9. SJL Model for Lymphoma Treatment.- 10. Gene Mapping and Molecular Cytogenetics Using Flow-Sorted Chromosomes.- 11. The Clinical Potential of DNA Content Parameters in Human Pediatric and Adult Solid Tumors.- 12. Differentiation of Human B-Cell Tumors: A Preclinical Model for Differentiation Therapy.- 13. Probing Immune Responses: The Role of Intra-Cellular Glutathione.- 14. DNA Topoisomerase II Poisons and the Cell Cycle.- 15. In Situ Hybridization of Centromeric DNA as Visualized on the Nuclear Matrix by Laser Confocal Cytometry.- 16. Megakaryocytopoiesis 2000.- 17. The Molecular Basis for the Control of Mammalian Cell growth.- 18. Isolation of Rare Cells by High-Speed Flow Cytometry and High-Resolution Cell Sorting for Subsequent Molecular Characterization - Application in Prenatal Diagnosis, Breast Cancer and Autologous Bone Marrow Transplantation.

Editorial Reviews

Reviewer: Charles Goolsby, PhD (Northwestern University Feinberg School of Medicine)
Description: This book is a partial compilation of presentations given at the 24th Detroit Cancer Symposium.
Purpose: The purpose of the book and the symposium is to examine the present application of analytical cytometry in the study of the basic biology and treatment of cancer.
Audience: This book does not in any way give a comprehensive review of the basic and clinical applications of flow cytometry techniques or clinical applications. This book is not for the novice or new researcher in the analytical cytometry arena looking for a text to provide a broad an overview of flow cytometry or the technical basics of flow cytometry applications. Many clinical applications are not even touched upon. However, for the experienced flow cytometry investigator using these techniques in cancer research, the book discusses a number of practical, theoretical, and philosophical problems and issues in selected areas of flow cytometry testing.
Features: In some instances, the treatment of the subject is somewhat brief and cursory, although in others it is thoughtful and pertinent. The black-and-white figures are adequate and clear.
Assessment: The book is a worthwhile contribution and is written by well-respected and competent researchers in the field. Although lacking in detail and breadth, the book tries to provide a historical perspective of flow cytometry studies in the cancer field, with lessons for the future in a nice chapter by Shapiro. The most interesting and exciting part of the book deals with the future in flow cytometry research and includes chapters on drug resistance measurements in solid tumors, gene mapping and molecular cytogenetics using flow sorted chromosomes, measurements of glutathione in immune response, in situ hybridization, cell cycle control, cell cycle poisons, and rare event analysis.

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