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Doody's Review ServiceReviewer: Bruce A. Fenderson, PhD (Thomas Jefferson University)
Description: This book introduces essential laboratory protocols for obtaining primary cultures of mammalian cells, authenticating cells in culture, and isolating specific types of differentiated cells. Its 24 chapters cover topics ranging from the hematopoietic development of embryonic stem cells to the differentiation of neurospheres. The principal focus of each chapter is on cell biology, developmental biology, and cell engineering.
Purpose: According to the editors, the objectives are to "provide the novice cell culturist with sufficient information to perform the basic techniques, to ensure the health and identity of their cell lines, and to be able to isolate and culture specialized primary cell types." Again, according to the editors, this book "is intended to offer clear methodologies pertinent to current areas of investigation, and to provide a valuable resource book for years to come.
Audience: This book is written for clinical and basic science researchers in the life sciences. The authors anticipate that readers will have a basic understanding of modern cell biology and biochemistry, and general familiarity with current research techniques. The authors are experts in this field.
Features: Useful step-by-step instructions for performing cell culture are provided. The editors have carefully selected interesting topics that are at the forefront of research in cellular and developmental biology, such as techniques for analyzing leukocyte adhesion and methods for inducing the differentiation of embryonic stem (ES) cells. Photomicrographs illustrate the morphology of various cell types in culture. The chapters include helpful tables, line drawings, and bibliographies. This book is accompanied by a CD-ROM that contains an e-book version of the information.
Assessment: This book is written by experienced authors who understand the practical needs of bench scientists. All laboratory personnel who perform cell culture on a regular basis will benefit from it. The authors provide summaries, keywords, helpful introductions, lists of necessary materials and equipment, and detailed protocols for culturing a wide-variety of cell types. The chapters on manipulating pluripotent stem cells will be of great value to researchers interested in regenerative medicine and cell engineering. This book is an important and valuable contribution to the field of cell biology and the art of manipulating cells in culture.