Basic Cell Culture Protocols / Edition 4

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At some point in their careers, virtually every scientist and technician, as well as many medical professionals, regardless of their area of specialization have a need to utilize cell culture systems. Updating and significantly expanding upon the previous editions, Basic Cell Culture Protocols, Fourth Edition provides the novice cell culturist with sufficient information to perform the basic techniques, to ensure the health and identity of their cell lines, and to be able to isolate and culture specialized primary cell types. The intent of this extensive volume is to generate a valuable resource containing clear methodologies pertinent to current areas of investigation, rather than attempting to educate cell culturists on specific cell types or organ systems. Written in the highly successful Methods in Molecular Biology, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Comprehensive and up-to-date, Basic Cell Culture Protocols, Fourth Edition compiles the essential techniques needed to approach this vital laboratory activity with full success.

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Editorial Reviews

Doody's Review Service
Reviewer: Bruce A. Fenderson, PhD (Thomas Jefferson University)
Description: This book introduces essential laboratory protocols for obtaining primary cultures of mammalian cells, authenticating cells in culture, and isolating specific types of differentiated cells. Its 24 chapters cover topics ranging from the hematopoietic development of embryonic stem cells to the differentiation of neurospheres. The principal focus of each chapter is on cell biology, developmental biology, and cell engineering.
Purpose: According to the editors, the objectives are to "provide the novice cell culturist with sufficient information to perform the basic techniques, to ensure the health and identity of their cell lines, and to be able to isolate and culture specialized primary cell types." Again, according to the editors, this book "is intended to offer clear methodologies pertinent to current areas of investigation, and to provide a valuable resource book for years to come."
Audience: This book is written for clinical and basic science researchers in the life sciences. The authors anticipate that readers will have a basic understanding of modern cell biology and biochemistry, and general familiarity with current research techniques. The authors are experts in this field.
Features: Useful step-by-step instructions for performing cell culture are provided. The editors have carefully selected interesting topics that are at the forefront of research in cellular and developmental biology, such as techniques for analyzing leukocyte adhesion and methods for inducing the differentiation of embryonic stem (ES) cells. Photomicrographs illustrate the morphology of various cell types in culture. The chapters include helpful tables, line drawings, and bibliographies. This book is accompanied by a CD-ROM that contains an e-book version of the information.
Assessment: This book is written by experienced authors who understand the practical needs of bench scientists. All laboratory personnel who perform cell culture on a regular basis will benefit from it. The authors provide summaries, keywords, helpful introductions, lists of necessary materials and equipment, and detailed protocols for culturing a wide-variety of cell types. The chapters on manipulating pluripotent stem cells will be of great value to researchers interested in regenerative medicine and cell engineering. This book is an important and valuable contribution to the field of cell biology and the art of manipulating cells in culture.

4 Stars! from Doody
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Product Details

  • ISBN-13: 9781627031271
  • Publisher: Springer-Verlag New York, LLC
  • Publication date: 11/20/2012
  • Series: Methods in Molecular Biology Series, #946
  • Edition description: 4th ed. 2013
  • Edition number: 4
  • Pages: 550
  • Product dimensions: 7.20 (w) x 10.10 (h) x 1.40 (d)

Table of Contents

1. Detection of Mycoplasma Contaminations

2. Eradication of Mycoplasma Contaminations

3. STR DNA Typing of Human Cell Lines: Detection of Intra- and Interspecies Cross-Contamination

4. Classical and Molecular Cytogenetic Analysis

5. Fluorescent In Situ Hybridization (FISH) of DNA Probes in the Interphase and Metaphase Stages of the Cell Cycle

6. The Development of T Lymphocytes in Fetal Thymus Organ Culture

7. Generation, Isolation, and Engraftment of In Vitro-Derived Human T Cell Progenitors

8. In Vitro Generation of Human T Regulatory Cells: Generation, Culture, and Analysis of FOXP3-Transduced T Cells

9. Simultaneous Cloning and Selection of Hybridomas and Transfected Cell Lines in Semisolid Media

10. Isolation and Characterization of Mouse Side Population Cells

11. Stem Cell Identification by DyeCycle Violet Side Population Analysis

12. Isolation and Characterization of Cancer Stem Cells In Vitro

13. Ex Vivo Differentiation of Cord Blood Stem Cells into Megakaryocytes and Platelets

14. Generation and Characterization of Murine Alternatively Activated Macrophages

15. Human Long Term Culture Initiating Cell (LTC-IC) Assay

16. Long Term Culture Initiating Cell (LTC-IC) Assay for Mouse Cells

17. Colony Forming Cell Assays for Human Hematopoietic Progenitor Cells

18. Studying Leukocyte Recruitment Under Flow Conditions

19. Generation and Establishment of Murine Adherent Cell Lines

20. Isolation, Enumeration, and Expansion of Human Mesenchymal Stem Cells in Culture

21. Isolation and Culture of Mesenchymal Stem Cells from Mouse Compact Bone

22. Generation of a Pool of Human Platelet Lysate (pHPL) and Efficient Use in Cell Culture

23. In Vitro Methods to Culture Primary Human Breast Epithelial Cells

24. Human Prostate Epithelial Cell Cultures

25. Enzymatic Dissociation, Flow Cytometric Analysis, and Culture of Normal Mouse Mammary Tissue

26. Isolation and Characterization of Human Hair Follicle Epithelial Cells

27. Co-Cultivation of Human Oral Keratinocytes and Human Osteoblast-Like Cells

28. Isolation and Culture of Skeletal Muscle Myofibers as a Means to Analyze Satellite Cells

29. Hepatic Differentiation of Embryonic Stem Cells by Murine Fetal Liver Mesenchymal Cells

30. Methods to Culture, Differentiate, and Characterize Neural Stem Cells from the Adult and Embryonic Mouse Central Nervous System

31. Feeder-Independent Culture Systems for Human Pluripotent Stem Cells

32. Formation of Embryoid Bodies from Human Pluripotent Stem Cells Using AggreWell™ Plates

33. Techniques in Embryoid Body Formation from Human Pluripotent Stem Cells

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