Basic Cell Culture Protocols / Edition 3

Basic Cell Culture Protocols / Edition 3

by Cheryl D. Helgason
     
 

ISBN-10: 1588292843

ISBN-13: 9781588292841

Pub. Date: 10/15/2004

Publisher: Springer-Verlag New York, LLC

Up-to-date and highly practical, Basic Cell Culture Protocols, Third Edition, offers basic scientists and clinician-researchers powerful tools to isolate, culture, and characterize the promising specialized cell types in demand today.  See more details below

Overview

Up-to-date and highly practical, Basic Cell Culture Protocols, Third Edition, offers basic scientists and clinician-researchers powerful tools to isolate, culture, and characterize the promising specialized cell types in demand today.

Product Details

ISBN-13:
9781588292841
Publisher:
Springer-Verlag New York, LLC
Publication date:
10/15/2004
Series:
Methods in Molecular Biology Series, #290
Edition description:
3rd ed. 2005
Pages:
371
Product dimensions:
5.98(w) x 9.02(h) x 0.35(d)

Table of Contents

1Culture of primary adherent cells and a continuously growing nonadherent cell line1
2Detection of mycoplasma contaminations13
3Eradication of mycoplasma contaminations25
4Authentication of scientific human cell lines : easy-to-use DNA fingerprinting35
5Cytogenetic analysis of cell lines51
6Human and mouse hematopoietic colony-forming cell assays71
7Isolation and culture of murine macrophages91
8Isolation and culture of human macrophages105
9Development of T-lymphocytes in mouse fetal thymus organ culture117
10In vitro generation of lymphocytes from embryonic stem cells135
11Hematopoietic development of human embryonic stem cells in culture149
12Generation of murine stromal cell lines : models for the microenvironment of the embryonic mouse aorta-gonads-mesonephros region163
13Culture of human and mouse mesenchymal cells173
14Isolation, purification, and cultivation of murine and human keratinocytes187
15Isolation and culture of primary human hepatocytes207
16Primary kidney proximal tubule cells231
17Enzymatic dissociation and culture of normal human mammary tissue to detect progenitor activity249
18Generation and differentiation of neurospheres from murine embryonic day 14 central nervous system tissue265
19Isolation and culture of skeletal muscle myofibers as a means to analyze-satellite cells281
20Adult ventricular cardiomyocytes : isolation and culture305
21Isolation and culture of primary endothelial cells315
22Studying leukocyte rolling and adhesion in vitro under flow conditions331
23Isolation and characterization of side population cells343
24Scalable production of embryonic stem cell-derived cells353

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