Basic Cell Culture Protocols / Edition 3

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Up-to-date and highly practical, Basic Cell Culture Protocols, Third Edition, offers basic scientists and clinician-researchers powerful tools to isolate, culture, and characterize the promising specialized cell types in demand today.
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Editorial Reviews

Doody's Review Service
Reviewer: Bruce A. Fenderson, PhD (Thomas Jefferson University)
Description: This book introduces essential laboratory protocols for obtaining primary cultures of mammalian cells, authenticating cells in culture, and isolating specific types of differentiated cells. Its 24 chapters cover topics ranging from the hematopoietic development of embryonic stem cells to the differentiation of neurospheres. The principal focus of each chapter is on cell biology, developmental biology, and cell engineering.
Purpose: According to the editors, the objectives are to "provide the novice cell culturist with sufficient information to perform the basic techniques, to ensure the health and identity of their cell lines, and to be able to isolate and culture specialized primary cell types." Again, according to the editors, this book "is intended to offer clear methodologies pertinent to current areas of investigation, and to provide a valuable resource book for years to come."
Audience: This book is written for clinical and basic science researchers in the life sciences. The authors anticipate that readers will have a basic understanding of modern cell biology and biochemistry, and general familiarity with current research techniques. The authors are experts in this field.
Features: Useful step-by-step instructions for performing cell culture are provided. The editors have carefully selected interesting topics that are at the forefront of research in cellular and developmental biology, such as techniques for analyzing leukocyte adhesion and methods for inducing the differentiation of embryonic stem (ES) cells. Photomicrographs illustrate the morphology of various cell types in culture. The chapters include helpful tables, line drawings, and bibliographies. This book is accompanied by a CD-ROM that contains an e-book version of the information.
Assessment: This book is written by experienced authors who understand the practical needs of bench scientists. All laboratory personnel who perform cell culture on a regular basis will benefit from it. The authors provide summaries, keywords, helpful introductions, lists of necessary materials and equipment, and detailed protocols for culturing a wide-variety of cell types. The chapters on manipulating pluripotent stem cells will be of great value to researchers interested in regenerative medicine and cell engineering. This book is an important and valuable contribution to the field of cell biology and the art of manipulating cells in culture.

4 Stars! from Doody
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Product Details

  • ISBN-13: 9781588295453
  • Publisher: Springer-Verlag New York, LLC
  • Publication date: 10/15/2004
  • Series: Methods in Molecular Biology Series, #290
  • Edition description: 3rd ed. 2005
  • Edition number: 3
  • Pages: 384
  • Product dimensions: 6.46 (w) x 8.94 (h) x 0.86 (d)

Table of Contents

1 Culture of primary adherent cells and a continuously growing nonadherent cell line 1
2 Detection of mycoplasma contaminations 13
3 Eradication of mycoplasma contaminations 25
4 Authentication of scientific human cell lines : easy-to-use DNA fingerprinting 35
5 Cytogenetic analysis of cell lines 51
6 Human and mouse hematopoietic colony-forming cell assays 71
7 Isolation and culture of murine macrophages 91
8 Isolation and culture of human macrophages 105
9 Development of T-lymphocytes in mouse fetal thymus organ culture 117
10 In vitro generation of lymphocytes from embryonic stem cells 135
11 Hematopoietic development of human embryonic stem cells in culture 149
12 Generation of murine stromal cell lines : models for the microenvironment of the embryonic mouse aorta-gonads-mesonephros region 163
13 Culture of human and mouse mesenchymal cells 173
14 Isolation, purification, and cultivation of murine and human keratinocytes 187
15 Isolation and culture of primary human hepatocytes 207
16 Primary kidney proximal tubule cells 231
17 Enzymatic dissociation and culture of normal human mammary tissue to detect progenitor activity 249
18 Generation and differentiation of neurospheres from murine embryonic day 14 central nervous system tissue 265
19 Isolation and culture of skeletal muscle myofibers as a means to analyze-satellite cells 281
20 Adult ventricular cardiomyocytes : isolation and culture 305
21 Isolation and culture of primary endothelial cells 315
22 Studying leukocyte rolling and adhesion in vitro under flow conditions 331
23 Isolation and characterization of side population cells 343
24 Scalable production of embryonic stem cell-derived cells 353
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