Benson's Microbiological Applications: Laboratory Manual in General Microbiology, Complete Version / Edition 11

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Overview

The classic resource for undergraduate microbiology laboratory courses just keeps getting better. The 78 self-contained, clearly illustrated exercises and full-color format makes Microbiological Applications: Laboratory Manual in General Microbiology, the ideal lab manual. Appropriate for either a majors or non-majors lab course, this manual assumes no prior organic chemistry course has been taken.
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Product Details

  • ISBN-13: 9780073522555
  • Publisher: McGraw-Hill Companies, The
  • Publication date: 1/16/2008
  • Format: Spiral Bound
  • Edition description: Older Edition
  • Edition number: 11
  • Pages: 560
  • Product dimensions: 9.40 (w) x 10.90 (h) x 1.20 (d)

Meet the Author

Professor and Chair, Consultant Environmental Microbiology
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Table of Contents

Part 1 Microscopy

Exercise 1 Brightfield Microscopy


Exercise 2 Darkfield Microscopy


Exercise 3 Phase-Contrast Microscopy


Exercise 4 Fluorescence Microscopy


Exercise 5 Microscopic Measurements

Part 2 Survey of Microorganisms


Exercise 6 Protozoans, Algae, and Cyanobacteria


Exercise 7 Ubiquity of Bacteria


Exercise 8 The Fungi: Yeasts and Molds

Part 3 Manipulation of Microorganisms


Exercise 9 Aseptic Technique


Exercise 10 Pure Culture Techniques

Part 4 Staining and Observation of Microorganisms


Exercise 11 Smear Preparation


Exercise 12 Simple Staining


Exercise 13 Negative Staining


Exercise 14 Capsular Staining


Exercise 15 Gram Staining


Exercise 16 Spore Staining: Two Methods


Exercise 17 Acid-Fast Staining: Ziehl-Neelsen Method


Exercise 18 Motility Determination

Part 5 Culture Methods


Exercise 19 Culture Media Preparation


Exercise 20 Preparation of Stock Cultures

Exercise 21 Cultivation of Anaerobes


Exercise 22 Enumeration of Bacteria: The Standard Plate Count


Exercise 23 Slime Mold Culture


Exercise 24 Slide Culture: Molds

Part 6 Bacterial Viruses


Exercise 25 Determination of a Bacteriophage Titer


Exercise 26 Burst Size Determination: A One-Step Growth Curve

Exercise 27 Isolation of Phage from Flies


Exercise 28 Phage Typing

Part 7 Enviornmental Influences and Control of Microbial Growth


Exercise 29 Temperature: Effects on Growth


Exercise 30 Temperature: Lethal Effects


Exercise 31 pH and Microbial Growth


Exercise 32 Water Activity and Osmotic Pressure


Exercise 33 Ultraviolet Light: Lethal Effects


Exercise 34 The Effects of Lysozyme on Bacterial Cells


Exercise 35 Evaluation of Alcohol: Its Effectiveness as an Antiseptic


Exercise 36 Antimicrobic Sensitivity Testing: The Kirby-Bauer Method


Exercise 37 Evaluation of Antiseptics: The Filter Paper Disk Method


Exercise 38 Effectiveness of Hand Scrubbing

Part 8 Identification of Unknown Bacteria


Exercise 39 Morphological Study of Unknown Bacterium


Exercise 40 Cultural Characteristics


Exercise 41 Physiological Characteristics: Oxidation and Fermentation Tests


Exercise 42 Physiological Characteristics: Hydrolytic and Degradative Reactions


Exercise 43 Physiological Characteristics: Multiple Test Media


Exercise 44 Use of Bergey's Manual

Part 9 Miniaturized Multitest Systems


Exercise 45 Enterobacteriaceae Identification: The API 20E System


Exercise 46 Enterobacteriaceae Identification: The Enterotube II System


Exercise 47 O/F Gram-Negative Rods Identification: The Oxi/Ferm Tube II System


Exercise 48 Staphyococcus Identification: The API Staph-Ident System

Part 10 Diversity and Environmental Microbiology


Exercise 49 Isolation of an Antibiotic Producer: The Actinomyces

Exercise 50 Nitrogen Cycle: Ammonification

Exercise 51 Symbiotic Nitrogen Fixation: Rhizobium

Exercise 52 Free-Living Nitrogen Fixation: Azotobacter

Exercise 53 Denitrification: Paracoccus Denitrificans

Exercise 54 The Winogradsky Column

Exercise 55 Purple Nonsulfer Photosynthetic Bacteria

Exercise 56 Sulfate Reducing Bacteria: Desulfovibrio

Exercise 57 Bacterial Commensalism

Exercise 58 Bacterial Synergism

Exercise 59 Microbial Antagonism
Part 11 Applied Microbiology

Exercise 60 Bacterial Counts of Foods


Exercise 61 Bacteriological Examination of Water: Qualitative Tests


Exercise 62 The Membrane Filter Method


Exercise 63 Reductase Test


Exercise 64 Microbial Spoilage of Canned Food


Exercise 65 Microbiology of Alcohol Fermentation

Part 12 Bacterial Genetics and Biotechnology


Exercise 66 Mutant Isolation by Replica Plating

Exercise 67 Bacterial Transformation

Exercise 68 Polymerase Chain Reaction for Amplifying DNA

Exercise 69 Plasmid Isolation
Part 13 Medical Microbiology and Immunology

Exercise 70 The Staphylococci: Isolation and Identification


Exercise 71 The Streptococci: Isolation and Identification


Exercise 72 Gram-Negative Intestinal Pathogens


Exercise 73 Slide Agglutination Test: Serological Typing


Exercise 74 Slide Agglutination (Latex) Test: For S. aureus Identification


Exercise 75 Tube Agglutination Test: The Heterophile Antibody Test


Exercise 76 White Blood Cell Study: The Differential WBC Count


Exercise 77 Blood Grouping


Exercise 78 A Synthetic Epidemic


Appendix A Tables

Appendix B Indicators, Stains, Reagents

Appendix C Media

Appendix D Identification Charts

Appendix E The Streptococci: Classification, Habitat, Pathology, and Biochemical Characteristics
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