Binding Constants: The Measurement of Molecular Complex Stability / Edition 1

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Overview

This unique book presents a systematic review of the methods for the determination of binding constants of complex formation in solution. Collects material that has been scattered throughout the literature of several separate fields. Offered here are methods from the areas of acid-base chemistry, metal-ion coordination compounds, hydrogen-bonding, charge-transfer complexation, hydrophobic interaction, and protein-binding. Discusses the relevant thermodynamics, modelling, statistics and regression analysis, and interpretation of data. Includes fresh discussions of random association (contact complexes), selection of standard states, and comparison of results. Treats all of the experimental methods useful for measuring these equilibrium constants, including those based on spectrophotometry, nuclear magnetic resonance, reaction kinetics, potentiometry, solubility, liquid-liquid partitioning, dialysis, chromatography, flourimetry, and many others.

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Product Details

  • ISBN-13: 9780471830832
  • Publisher: Wiley
  • Publication date: 5/4/1987
  • Edition number: 1
  • Pages: 432
  • Product dimensions: 6.12 (w) x 9.31 (h) x 1.11 (d)

Table of Contents

GENERAL MATTERS.

Molecular Association.

Binding Constants.

Statistical Treatment of Data.

THE METHODS.

Optical Absorption Spectroscopy.

Magnetic Resonance Spectroscopy.

Reaction Kinetics.

Potentiometry.

Solubility Measurement.

Interpretation of Solubility Diagrams.

Liquid-Liquid Partitioning.

Dialysis.

Chromatography.

Additional Methods.

The Method of Choice.

Appendixes.

Index.

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