Bioanalytical Chemistry / Edition 1

Bioanalytical Chemistry / Edition 1

by Susan R. Mikkelsen, Eduardo Corton
     
 

A timely, accessible survey of the multidisciplinary field of bioanalytical chemistry

Bioanalytical chemistry is playing an increasingly central role in the fields of academic and industrial science. It overlaps with a diverse range of disciplines, including biotechnology, biopharmaceuticals, and diagnostics–all of which makes a comprehensive

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Overview

A timely, accessible survey of the multidisciplinary field of bioanalytical chemistry

Bioanalytical chemistry is playing an increasingly central role in the fields of academic and industrial science. It overlaps with a diverse range of disciplines, including biotechnology, biopharmaceuticals, and diagnostics–all of which makes a comprehensive introduction to the subject even more essential for students and researchers.

Featuring many of the techniques used by biochemists and molecular biologists, Bioanalytical Chemistry reviews all relevant aspects of using analytical methodology to solve biological problems. The end result is an authoritative primer to this expanding field–one that will prove invaluable for advanced undergraduate chemistry and biochemistry students, bioanalytical chemistry graduate students, and practitioners. Each chapter in the book begins with coverage of basic concepts, then progresses to applications that involve real samples.

  • Bioanalytical Chemistry covers:
  • Spectroscopic methods for matrix characterization
  • Structural and functional properties of antibodies
  • Principles of electrophoresis
  • Centrifugation methods
  • Mass spectrometry of biomolecules
  • Quantitation of enzymes and their substrates
  • Design and implementation of enzyme assays
  • Isoelectric focussing
  • Chromatography of biomolecules
  • Validation of new bioanalytical methods
  • And much more

This indispensable guide features end-of-chapter problems and selected answers in the appendix, designed to help students assess their knowledge of each topic. Each chapter also includes suggestions for further reading, and mathematical derivations have been kept to a minimum throughout.

More than any other reference, Bioanalytical Chemistry captures the interdisciplinary scope of this emerging field–and uniquely prepares students for collaborative scientific work and research in industrial, government, and academic laboratories.

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Product Details

ISBN-13:
9780471544470
Publisher:
Wiley
Publication date:
03/02/2004
Edition description:
New Edition
Pages:
384
Product dimensions:
6.48(w) x 9.39(h) x 1.11(d)

Table of Contents

Preface.

Acknowledgments.

1. Spectroscopic Methods for Matrix Characterization.

2. Enzymes.

3. Quantitation of Enzymes and Their Substrates.

4. Immobilized Enzymes.

5. Antibodies.

6. Quantitative Immunoassays with Labels.

7. Biosensors.

8. Directed Evolution for the Design of Macromolecular Bioassay Reagents.

9. Principles of Electrophoresis.

10. Applications of Zone Electrophoresis.

11. Isoelectric Focusing.

12. Capillary Electrophoresis.

13. Centrifugation Methods.

14. Chromatography of Biomolecules.

15. Mass Spectrometry of Biomolecules.

16. Validation of New Bioanalytical Methods.

Suggested References.

References.

Answers to Problems.

Index.

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