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From The CriticsReviewer: Richard L. Clancy, PhD (University of Kansas Medical Center)
Description: This book describes techniques for preparing myocardial sarcoplasmic reticulum, sarcolemma, and cardiac myocytes. The remaining chapters are devoted to illustrating how these preparations are used to measure transmembrane ion fluxes.
Purpose: The objective is to provide a "recipe book" for the investigator who has little or no experience in using these types of cardiac cell and subcellular preparations. The fact that methodology sections of most peer-reviewed articles are by necessity brief makes this a valuable book. The completeness of the descriptions will aid the investigator in developing and using any one of these techniques.
Audience: The descriptions are readily comprehensible for the laboratory assistant, the student, and the senior investigator. All the authors have extensive experience in using these myocyte preparations.
Features: Flow charts illustrating techniques for isolating cell and subcellular systems and tables detailing composition of solutions and assays are used very effectively. Each chapter has a sizable number of current and relevant references. Of concern are several pages in which the print is out of focus.
Assessment: This book is very useful to the investigator who has not had any experience using any one of these cardiac cell preparations. The book provides a comprehensive yet succinct description of how to isolate myocardial cells and cellular components, the equipment, supplier and solutions needed for performing experiments using these preparations, and the advantages and disadvantages of each type of preparation. Biomedical libraries should be encouraged to purchase a copy of this book.