Biochemistry / Edition 1

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Ideal for USMLE preparation and course review, the streamlined, easy-to-follow hierarchical outline format guides students through the most important aspects of each topic. The text is extensively illustrated to convey difficult-to-understand concepts. Clinical correlations, numerous tables and charts, and USMLE-style questions in clinical vignette format help students evaluate their strengths and weaknesses.

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Product Details

  • ISBN-13: 9780071442053
  • Publisher: McGraw-Hill Companies, The
  • Publication date: 7/15/2007
  • Series: LANGE USMLE Road Maps Series
  • Edition description: New Edition
  • Edition number: 1
  • Pages: 224
  • Product dimensions: 7.38 (w) x 9.26 (h) x 0.35 (d)

Meet the Author

Richard G. MacDonald, PhD


Department of Biochemistry and Molecular Biology

University of Nebraska Medical Center

Omaha, NE

William G. Chaney, PhD

Associate Professor

Department of Biochemistry and Molecular Biology

University of Nebraska Medical Center

Omaha, NE

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Table of Contents

1. Physiological Chemistry and Bonding
A. Water as biochemical solvent
B. Hydrogen bonding
C. pH and buffers
D. Other non-covalent interactions
E. Fat-water interfaces
2. Protein Structure and Function
A. Amino acids
B. Primary structure—protein sequence
C. Secondary structures—-helix and -sheet
D. Tertiary structure—domains
E. Quaternary structure—subunits
F. Fibrous proteins—collagen and elastin
G. Globular proteins—myoglobin and hemoglobin
H. Folding and chaperones
3. Enzyme Structure and Function
A. Types of enzymes—classification
B. Kinetics—Michaelis-Menten enzymes
C. Inhibition
D. Allosteric regulation
E. Mechanisms—chymotrypsin and ribonuclease
4. DNA Structure and Function
A. Purine and pyrimidine bases
B. The DNA molecule
C. Packaging into chromatin
D. Replication
E. Recombination and repair
5. RNA Structure and Function
A. RNA structures—mRNA, rRNA, tRNA
B. Transcription
C. Splicing
D. Ribonucleoprotein complexes
6. DNA and Chromosome Analysis
A. Hybridization
B. Nucleic acid electrophoresis
C. Northern and Southern blotting
D. RFLP analysis
E. Polymerase chain reaction
F. Allele-specific oligonucleotides
G. Fluorescence in situ hybridization
7. Control of Gene Expression
A. The lac operon
B. The Trp operon
C. Eukaryotic promoters, enhancers and repressors
D. Transcription factors
E. Methylation and acetylation
8. Protein Biosynthesis, degradation and trafficking
A. Translation
B. Post-translational modification
C. Secretion
D. Intracellular targeting
E. Lysosomal degradation
F. Proteosomal degradation
9. Biological Membranes
A. The lipid bilayer
B. Transport
C. Junctions and intercellular communication
10. Cytoskeleton and Extracellular Matrix
A. Microtubules and microfilaments
B. Integrins and focal adhesions
C. Extracellular matrix proteins
D. Proteoglycans and glycosaminoglycans
E. Motility
11. Overview of Metabolic Relationships
A. Digestion of nutrients
B. Catabolism vs. anabolism
C. Carbohydrates
D. Lipids
12. Carbohydrate Metabolism
A. Glycolysis
B. Anaerobic glycolysis
C. Aerobic glycolysis
D. Pentose Phosphate Pathway
13. Hexose Regulation
A. Gluconeogenesis
B. Glycogen biosynthesis and glycogenolysis
C. Hexose metabolism
14. Pyruvate Oxidation
A. Biosynthesis of Acetyl-CoA
B. Steps of the TCA Cycle
C. Regulation of Pyruvate dehydrogenase
D. Regulation of the TCA cycle
15. Oxidative Phosphorylation
A. Transfer of electrons to Complex I
B. Delivery of electrons through Complex II
C. Ubiquinone and common steps in electron transfer
D. Creation of a pH gradient during electron transfer
E. Utilization of the pH gradient to form ATP
F. Inhibitors of ATP biosynthesis
G. Stoichiometry of ATP biosynthesis
16. Lipid Metabolism
A. Fatty acid biosynthesis
B. Fatty acid degradation
C. Formation of triglycerides
D. Glycolipid biosynthesis
E. Eicosanoid biosynthesis
F. Cholesterol Biosynthesis
G. Lipoproteins
17. Amino Acid Metabolism
A. Nitrogen metabolism
B. Essential amino acids
C. Catabolism of amino acids
D. Biosynthesis of amino acids
E. Biosynthesis of porphyrins
18. Nucleic Acid Metabolism
A. Biosynthesis of purines
B. Biosynthesis of pyrimidines
C. Biosynthesis of deoxynucleotides
D. Salvage pathways of bases
E. Degradation of purines
F. Degradation of pyrimidines
19. Nutritional Biochemistry
A. General Nutritional Requirements
B. Vitamins
1. Water-soluble
2. Fat-soluble
C. Essential inorganic nutrients
D. Essential amino acids
E. Essential lipids
20. Receptors and Signal Transduction
A. Overview of receptors and ligands
B. Types of receptors
C. Nuclear receptor superfamily
D. Membrane receptors
1. G protein-coupled receptors
2. Growth factor receptors
E. Nitric oxide
F. Protein kinase A
G. Protein kinase C
H. Calcium-calmodulin signaling
I. Kinase cascades
21. Cancer Biology
A. Overview of cancer as a genetic disease
B. Definitions
C. Chemical carcinogenesis
D. Radiation-induced DNA damage
1. Ionizing radiation—x-rays, gamma rays
2. Ultraviolet radiation
E. Oncogene activation
F. Tumor suppressors
G. Programmed cell death—apoptosis

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