Bioconjugate Techniques / Edition 2

Bioconjugate Techniques / Edition 2

5.0 2
by Greg T. Hermanson

ISBN-10: 0123705010

ISBN-13: 9780123705013

Pub. Date: 04/01/2008

Publisher: Elsevier Science

Essential guide to the modification and crosslinking of biomolecules for use in research, diagnostics, and therapeutics.  See more details below


Essential guide to the modification and crosslinking of biomolecules for use in research, diagnostics, and therapeutics.

Product Details

Elsevier Science
Publication date:
Edition description:
Older Edition
Product dimensions:
7.50(w) x 9.20(h) x 1.80(d)

Table of Contents

Pt. I Bioconjugate Chemistry

1 Functional Targets 3

2 The Chemistry of Reactive Groups 169

Pt. II Bioconjugate Reagents

3 Zero-Length Crosslinkers 215

4 Homobifunctional Crosslinkers 234

5 Heterobifunctional Crosslinkers 276

6 Trifunctional Crosslinkers 336

7 Dendrimers and Dendrons 346

8 Cleavable Reagent Systems 391

9 Fluorescent Probes 396

10 Brfunctional Chelating Agents and Radioimmunoconjugates 498

11 Biotinylation Reagents 506

12 Iodination Reagents 546

13 Silane Coupling Agents 562

14 Microparticles and Nanoparticles 582

15 Buckyballs, Fullerenes, and Carbon Nanotubes 627

16 Mass Tags and Isotope Tags 649

17 Chemoselective Ligation: Bioorthogonal Reagents 666

18 Discrete PEG Reagents 707

Pt. III Bioconjugate Applications

19 Preparation of Hapten-Carrier lmmunogen Conjugates 745

20 Antibody Modification and Conjugation 783

21 Immunotoxin Conjugation Techniques 824

22 Preparation of Liposome Conjugates and Derivatives 858

23 Avidin-Biotin Systems 900

24 Preparation of Colloidal Gold-Labeled Proteins 924

25 Modification with Synthetic Polymers 936

26 Enzyme Modification and Conjugation 961

27 Nucleic Acid and Oligonucleotide Modification and Conjugation 969

28 Bioconjugation in the Study of Protein Interactions 1003

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Bioconjugate Techniques 5 out of 5 based on 0 ratings. 2 reviews.
Anonymous More than 1 year ago
I use this book at least weekly, and often more frequently than that. Greg Hermanson has written the best and most useful reference in the area of bioconjugation that I have found. His discussion of the chemistry is well-researched, clearly presented, accurate, and easy to understand. His laboratory protocols are excellent in themselves and easy to adapt to virtually any need. The references alone are almost worth the price of the book. Overall, if you are involved in bioconjugation, this book is your MUST HAVE reference. I just wish this book was available as an e-book through Barnes and Noble.
Anonymous More than 1 year ago
This second edition covers all reagents for chemical syntheses and more than 1800 references, constituting a comprehensive source for original designs of many varieties of bioconjugates. Extensive interactions between vital function groups and biological molecules are exceptionally complicated but are well deciphered in Parts II and III and thereafter are capable generating infinite novel entities to fulfill unmet therapeutic applications by assorted professionals. Well-schemed chemical reactions followed by protocols across all subjects are neat and intuitive, presenting two exceptional values for readers: generating inspiration and hands-on modus operandi. This book would definitely stimulate people who plan or already pursue careers in medical, biosensor, pharmaceutics, bioengineering, diagnostics, biochemistry, and microbiological industries.