Bioconjugate Techniques / Edition 2

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Overview

Essential guide to the modification and crosslinking of biomolecules for use in research, diagnostics, and therapeutics.

Audience: Researchers in biochemistry, molecular biology, immunology, pharmacology, cell biology, and microbiology will be interested.

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Editorial Reviews

Walter D. Niles
Bioconjugation is the covalent linking together of biomolecules to produce a hybrid molecule with properties of both its precursors. This book is a compendium of methodologies, recipes, and applications of bioconjugation that have produced useful molecules. This is a useful book with lots of practical knowledge, a good index, and an adequate list of references to the primary literature. Bioconjugated molecules have found places in nearly all fields of the modern life science, ranging from new reagents for understanding basic mechanisms, to diagnostic tools and exciting new therapeutics at the very edge of biotechnological research and development. The author has succeeded in the formidable task of collecting and presenting, in accessible and practical ways, the common chemical foundations for a diverse assortment of bioconjugations. This book will find a place in the library or on the reading stand of all people interested in this area including bench scientists (students or seasoned veterans) in academic and industrial research, patent lawyers, perhaps, or anyone seeking to get a handle on this burgeoning field. Even the senior professional who has contributed to the primary literature may appreciate having a fairly large amount of information in one place. The author himself is in the thick of bioconjugation products at Pierce Chemical Company in Rockford, IL, with a track record in haptogen creation and in biotinylation, and he has provided a great service by relating the do's, don'ts, and possible blind alleys and sidetracks gained from both personal experience as well as collective wisdom. The book is not particularly biased toward Pierce's products; the BODIPY line of newfluorescent dyes produced by at least one competitor, Molecular Probes, Inc. of Beaverton, OR, is covered. Beginning with an overview of the structures of proteins, nucleic acids, lipids, and carbohydrates and their reactive functional groups, the first part of the book emphasizes reaction mechanisms. The second section covers a broad assortment of available reactive compounds. These reagents are considered both from the perspective of the functional groups they modify and then from the types of reaction products they generate, in an especially comprehensive section. Containing not only detailed protocols useful for particular conjugation reactions, it also considers possible pitfalls and side reactions and offers cautionary procedures for improving chances of desired results. The final part of the book covers some very specific applications such as the generation of immunogenic haptens, hybrid antibody molecule formation with a detailed treatment of immunotoxins, attachment of a variety of functional moieties to lipids to make special liposomes for drug delivery, and the labeling of nucleic wills with fluorescent and active probes. The only potential problems with this book might be currency (nearly all available fluorescent probes are covered, with one notable exception -- the cyanine dyes that are rapidly growing their utility in generating fluorescent antibodies), the odd reference that is not especially helpful, or an omitted reference that demystifies a field (such as liposome formation). These are trivial quibbles, of course, with a generally magnificent work that covers such a broad area and packs in a great many useful details. I am glad that someone finally wrote this book. Bravo!
From The Critics
Reviewer: Jed N. Lampe, Ph.D.(University of Kansas Medical Center)
Description: This book has served as the de facto bible for bioconjugate chemists since the first edition in 1996. This third edition, updated with all new color figures, continues to set the standard as a comprehensive guide for researchers wishing to conjugate their favorite protein or nucleic acid to a label or chemical handle.
Purpose: The author's goal is to distill the vast field of bioconjugate chemistry down to three core areas — chemistry, reagent systems, principal applications — and then cover each of them in depth. While providing a practical overview, this book also serves as a comprehensive methods manual that both veteran and novice researchers can use effectively. The complete, easy-to-follow protocols make this a wonderful bench top laboratory reference in its own right.
Audience: It is directed at any research chemist, biochemist, or student who has ever had the problem of wanting to track a protein or nucleic acid in a biological milieu or cell extract but has lacked the tools and knowledge to do so. Here they will find all the information necessary to prepare, conjugate, and purify a molecule of interest with a minimal expenditure of time and resources. The author has poured a career worth of experience into a tidy, efficient bench top manual.
Features: New in this edition are entirely updated color figures, an introductory overview chapter on bioconjugation, and a chapter with an extensive discussion of immobilization chemistry, critical for purifying any conjugated biomolecule. Readers will enjoy the succinct, straightforward protocols as well as the brief introductions at the beginning of each chapter and liberal references for each technique.
Assessment: This is an incomparable and essential guide for any scientist involved in conjugating biologically active molecules. The new edition does not disappoint with its liberal use of color (especially in reaction schemes), expanded pages, and newly added chapters. For those interested in bioconjugate chemistry, you may soon find that this is the only book on your research library shelf!
From the Publisher

"Most of the chapters address specific systems and reagents such as (strept) avidin-biotin, silane coupling, buckyballs & fullerenes, antibodies, liposome conjugates, and modified nucleotides-as well as a few chapters on observation techniques such as fluorescent probes and isotopic labeling. Also new since the last edition is a chapter addressing immobilization in chromatography."--ProtoView.com, February 2014 "This is an incomparable and essential guide for any scientist involved in conjugating biologically active molecules. The new edition does not disappoint with its liberal use of color (especially in reaction schemes), expanded pages, and newly added chapters. For those interested in bioconjugate chemistry, you may soon find that this is the only book on your research library shelf!" Rating: 5 Stars--Doody.com, February 7, 2014


4 Stars! from Doody
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Product Details

  • ISBN-13: 9780123705013
  • Publisher: Elsevier Science
  • Publication date: 4/1/2008
  • Edition description: Older Edition
  • Edition number: 2
  • Pages: 1323
  • Product dimensions: 7.50 (w) x 9.20 (h) x 1.80 (d)

Table of Contents

Pt. I Bioconjugate Chemistry

1 Functional Targets 3

2 The Chemistry of Reactive Groups 169

Pt. II Bioconjugate Reagents

3 Zero-Length Crosslinkers 215

4 Homobifunctional Crosslinkers 234

5 Heterobifunctional Crosslinkers 276

6 Trifunctional Crosslinkers 336

7 Dendrimers and Dendrons 346

8 Cleavable Reagent Systems 391

9 Fluorescent Probes 396

10 Brfunctional Chelating Agents and Radioimmunoconjugates 498

11 Biotinylation Reagents 506

12 Iodination Reagents 546

13 Silane Coupling Agents 562

14 Microparticles and Nanoparticles 582

15 Buckyballs, Fullerenes, and Carbon Nanotubes 627

16 Mass Tags and Isotope Tags 649

17 Chemoselective Ligation: Bioorthogonal Reagents 666

18 Discrete PEG Reagents 707

Pt. III Bioconjugate Applications

19 Preparation of Hapten-Carrier lmmunogen Conjugates 745

20 Antibody Modification and Conjugation 783

21 Immunotoxin Conjugation Techniques 824

22 Preparation of Liposome Conjugates and Derivatives 858

23 Avidin-Biotin Systems 900

24 Preparation of Colloidal Gold-Labeled Proteins 924

25 Modification with Synthetic Polymers 936

26 Enzyme Modification and Conjugation 961

27 Nucleic Acid and Oligonucleotide Modification and Conjugation 969

28 Bioconjugation in the Study of Protein Interactions 1003

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Customer Reviews

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Sort by: Showing all of 2 Customer Reviews
  • Anonymous

    Posted October 16, 2010

    The Best Book of Its Kind

    I use this book at least weekly, and often more frequently than that. Greg Hermanson has written the best and most useful reference in the area of bioconjugation that I have found. His discussion of the chemistry is well-researched, clearly presented, accurate, and easy to understand. His laboratory protocols are excellent in themselves and easy to adapt to virtually any need. The references alone are almost worth the price of the book. Overall, if you are involved in bioconjugation, this book is your MUST HAVE reference. I just wish this book was available as an e-book through Barnes and Noble.

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  • Anonymous

    Posted February 12, 2009

    I Also Recommend:

    Chemistry and advanced biotechnology collide to create innovative bioconjagates of many types.

    This second edition covers all reagents for chemical syntheses and more than 1800 references, constituting a comprehensive source for original designs of many varieties of bioconjugates. Extensive interactions between vital function groups and biological molecules are exceptionally complicated but are well deciphered in Parts II and III and thereafter are capable generating infinite novel entities to fulfill unmet therapeutic applications by assorted professionals. Well-schemed chemical reactions followed by protocols across all subjects are neat and intuitive, presenting two exceptional values for readers: generating inspiration and hands-on modus operandi. This book would definitely stimulate people who plan or already pursue careers in medical, biosensor, pharmaceutics, bioengineering, diagnostics, biochemistry, and microbiological industries.

    Was this review helpful? Yes  No   Report this review
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