Bioconjugation Protocols: Strategies and Methods / Edition 1

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Expert laboratorians update the classic bioconjugation methods and introduce valuable new techniques that go beyond pure biomedical applications to include elements from advanced organic synthesis, molecular biology, and materials science. These readily reproducible methods cover the preparation of protein conjugates using covalent and noncovalent conjugation, the synthesis of nucleic acid conjugates using a variety of labeling techniques, and approaches to semisynthetic conjugates of proteins. Additional chapters address the biofunctionalization of inorganic surfaces, including the on-chip synthesis of peptide nucleic acids to generate microarrays for the high-throughput analysis of RNA and DNA, gold nanaoparticles, and carbon nanotube probes for atomic force microscopy.

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Product Details

  • ISBN-13: 9781588290984
  • Publisher: Springer-Verlag New York, LLC
  • Publication date: 7/16/2004
  • Series: Methods in Molecular Biology Series, #283
  • Edition number: 1
  • Pages: 344
  • Product dimensions: 6.22 (w) x 9.30 (h) x 0.95 (d)

Table of Contents

Part I. Antibody and Enzyme Conjugates

Streptavidin-Biotin Crosslinking of Therapeutic Enzymes With Carrier Antibodies: Nanoconjugates for Protection Against Endothelial Oxidative Stress

Vladimir V. Shuvaev, Thomas Dziubla, Rainer Wiewrodt, and Vladimir R. Muzykantov

Characterization of Endothelial Internalization and Targeting of Antibody-Enzyme Conjugates in Cell Cultures and in Laboratory Animals

Silvia Muro, Vladimir R. Muzykantov, and Juan-Carlos Murciano

Smart Polymer-Streptavidin Conjugates

Patrick S. Stayton, Zhongli Ding, and Allan S. Hoffman

Conjugates of Peptides and Proteins to Polyethylene Glycols

Margherita Morpurgo and Francesco M. Veronese

Chemical Production of Bispecific Antibodies

Robert F. Graziano and Paul Guptill

Preparation of Immunoconjugates Using Antibody Oligosaccharide Moieties

Carl-Wilhelm Vogel

Synthesis of Hapten-Protein Conjugates Using Microbial Transglutaminase

Markus Meusel

Part II. Nucleic Acid Conjugates

Fluorescent Sample Labeling for DNA Microarray Analyses

Verena Beier, Andrea Bauer, Michael Baum, and Jörg D. Hoheisel

High-Density Labeling of DNA for Single Molecule Sequencing

Susanne Brakmann

Sequence-Specific DNA Labeling Using Methyltransferases

Goran Pljevaljcic, Falk Schmidt, Alexander Peschlow, and Elmar Weinhold

Hapten Labeling of Nucleic Acids for Immuno-Polymerase Chain Reaction Applications

Michael Adler

Covalent Coupling of DNA Oligonucleotides and Streptavidin

Florian Kukolka, Marina Lovrinovic, Ron Wacker, and Christof M. Niemeyer

Synthesis of Oligonucleotide-Peptide and Oligonucleotide-Protein Conjugates

David R. Corey

Synthesis of Peptide Nucleic Acid-Peptide Conjugates

Kunihiro Kaihatsu and David R. Corey

Part III. Glycosyl and Lipid Conjugates

Protein Lipidation

Jürgen Kuhlmann

Synthesis of Lipidated Peptides

Ines Heinemann, Martin Völkert, and Herbert Waldmann

In Vitro Semisynthesis and Applications of C-Terminally Modified Rab Proteins

Thomas Durek, Roger S. Goody, and Kirill Alexandrov

Generation and Characterization of Ras Lipoproteins Based on Chemical Coupling

Melanie Wagner and Jürgen Kuhlmann

Conjugation of Glycopeptide Thioesters to Expressed Protein Fragments: Semisynthesis of Glycosylated Interleukin-2

Thomas J. Tolbert and Chi-Huey Wong

Subtilisin-Catalyzed Glycopeptide Condensation

Thomas J. Tolbert and Chi-Huey Wong

Part IV. Biofunctionalization of Surfaces

Peptide Nucleic Acid Microarrays

Anette Jacob, Ole Brandt, Achim Stephan, and Jörg D. Hoheisel

Synthesis and Characterization of Deoxyribonucleic Acid-Conjugated Gold Nanoparticles

Pompi Hazarika, Tatiana Giorgi, Martina Reibner, Buelent Ceyhan, and Christof M. Niemeyer

Biofunctionalization of Carbon Nanotubes for Atomic Force Microscopy Imaging

Adam T. Woolley


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