Biolistic DNA Delivery: Methods and Protocols

Overview

Biolistic transfection represents a direct physical gene transfer approach in which nucleic acids are precipitated on biologically inert high-density microparticles (usually gold or tungsten) and delivered directly through cell walls and/or membranes into the nucleus of target cells by high-velocity acceleration using a ballistic device such as the gene gun. Biolistic DNA Delivery: Methods and Prools provides a comprehensive collection of detailed prools intended to provide the definitive practical guide for the ...

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Overview

Biolistic transfection represents a direct physical gene transfer approach in which nucleic acids are precipitated on biologically inert high-density microparticles (usually gold or tungsten) and delivered directly through cell walls and/or membranes into the nucleus of target cells by high-velocity acceleration using a ballistic device such as the gene gun. Biolistic DNA Delivery: Methods and Prools provides a comprehensive collection of detailed prools intended to provide the definitive practical guide for the novice as well as for the advanced gene transfer expert on how to introduce nucleic acids into eukaryotic cells using the biolistic technique. Split into six convenient sections, this detailed volume covers biolistic gene transfer into plants, nematodes, and mammalian cells, both in vitro and in vivo, as well as the use of gene gun-mediated DNA vaccination in various experimental animal models of human diseases, and the description of biolistic delivery of molecules other than nucleic acids. Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory prools, and tips on troubleshooting and avoiding known pitfalls.

All-inclusive and cutting-edge, Biolistic DNA Delivery: Methods and Prools brings together the knowledge and the experience of leading experts in the field of gene transfer in order to serve all researchers who wish to further our abilities in this vital field.

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Product Details

  • ISBN-13: 9781627031097
  • Publisher: Springer-Verlag New York, LLC
  • Publication date: 10/29/2012
  • Series: Methods in Molecular Biology Series, #940
  • Edition description: 2013
  • Edition number: 1
  • Pages: 415
  • Product dimensions: 7.00 (w) x 10.00 (h) x 1.20 (d)

Table of Contents

Part I: Biolistic DNA Delivery in Plant Science and Agricultural Biotechnology

1. Comparison Between Agrobacterium-Mediated and Direct Gene Transfer Using the Gene Gun
Caixia Gao and Klaus K. Nielsen

2. Transient Gene Expression in Epidermal Cells of Plant Leaves by Biolistic DNA Delivery
Shoko Ueki, Shimpei Magori, Benoît Lacroix, and Vitaly Citovsky

3. Transformation of Nuclear DNA in Meristematic and Embryogenic Tissues Mulpuri Sujatha and K.B.R.S. Visarada

4. Biolistic DNA Delivery to Leaf Tissue of Plants with the Non-Vacuum Gene Gun (HandyGun) Anssi L. Vuorinen, Arto Nieminen, Victor Gaba, Sidona Sikorskaite, and Jari P.T. Valkonen

5. HandGun-Mediated Inoculation of Plants with Viral Pathogens for Mechanistic Studies Victor Gaba, Moshe Lapidot, and Amit Gal-On

6. Biolistics-Based Gene Silencing in Plants Using a Modified Particle Inflow Gun Kevin M. Davies, Simon C. Deroles, Murray R. Boase, Don A. Hunter, and Kathy E. Schwinn

Part II: Biolistic DNA Delivery in Nematodes

7. Biolistic Transformation of Caenorhabditis elegans Meltem Isik and Eugene Berezikov

8. Improved Vectors for Selection of Transgenic Caenorhabditis elegans Annabel A. Ferguson, Liquan Cai, Luv Kashyap, and Alfred L. Fisher

9. Biolistic Transformation of Brugia malayi Tarig B. Higazi and Thomas R. Unnasch

Part III: Biolistic DNA Delivery in Mammalian Cells In Vitro

10. Biolistic Transfection of Human Embryonic Kidney (HEK) 293 Cells Xiongwei Li, Masaki Uchida, H. Oya Alpar, and Peter Mertens

11. Biolistic Transfection of Tumor Tissue Samples Kandan Aravindaram, Shu-Yi Yin, and Ning-Sun Yang

12. Biolistic Transfection of Freshly Isolated Adult Ventricular Myocytes David F. Steele, Ying Dou, and David Fedida

13. Biolistic Transfection of Neurons in Organotypic Brain Slices John A. O’Brien and Sarah C.R. Lummis

Part IV: Biolistic DNA Delivery in Mammalian Cells In Vivo
14. Biolistic DNA Delivery to Mice with the Low Pressure Gene Gun Meng-Chi Yen and Ming-Derg Lai

15. Chemokine Overexpression in the Skin by Biolistic DNA Delivery Ahmad Jalili

16. Enhancement of Gene Gun-Induced Vaccine-Specific Cytotoxic T Cell Response by Administration of Chemotherapeutic Drugs Steve Pascolo

17. Dendritic Cell-Specific Biolistic Transfection Using the Fascin Gene Promoter Yvonne Höhn, Stephan Sudowe, and Angelika B. Reske-Kunz

18. Particle-Mediated Administration of Plasmid DNA on Corneas of BALB/c Mice Dirk Bauer, Susanne Wasmuth, Mengji Lu, and Arnd Heiligenhaus

Part V: Biolistic DNA Vaccination in Animal Disease Models

19. Optimizing Particle-Mediated Epidermal Delivery of an Influenza DNA Vaccine in Ferrets Eric J. Yager, Cristy Stagnar, Ragisha Gopalakrishnan, James T. Fuller, and Deborah H. Fuller

20. Methods for Monitoring Gene Gun-Induced HBV- and HCV-Specific Immune Responses in Mouse Models Gustaf Ahlén, Matti Sällberg, and Lars Frelin

21. Gene Gun Immunization to Combat Malaria Elke S. Bergmann-Leitner and Wolfgang W. Leitner

22. Identification of T-cell Epitopes of Mycobacterium tuberculosis with Biolistic DNA Vaccination Toshi Nagata and Yukio Koide

23. Biolistic DNA Vaccination Against Trypanosoma Infection Marianne Bryan, Siobhan Guyach, and Karen A. Norris

24. Biolistic DNA Vaccination Against Melanoma Julia Steitz and Thomas Tüting

25. Biolistic DNA Vaccination Against Cervical Cancer Michal Šmahel

26. Efficiency of Biolistic DNA Vaccination in Experimental Type I Allergy Verena Raker, Joachim Maxeiner, Angelika B. Reske-Kunz, and Stephan Sudowe

27. Safety Assessment of Biolistic DNA Vaccination Barbara Langer, Matthias Renner, Jürgen Scherer, Silke Schüle, and Klaus Cichutek

Part VI: Related Applications for Biolistic Delivery of Molecules

28. DiOlistics: Delivery of Fluorescent Dyes into Cells Nyssa Sherazee and Veronica A. Alvarez

29. Protein Antigen Delivery by Gene Gun-Mediated Epidermal Antigen Incorporation (EAI) Sandra Scheiblhofer, Uwe Ritter, Josef Thalhamer, and Richard Weiss

Mulpuri Sujatha and K.B.R.S. Visarada

4. Biolistic DNA Delivery to Leaf Tissue of Plants with the Non-Vacuum Gene Gun (HandyGun) Anssi L. Vuorinen, Arto Nieminen, Victor Gaba, Sidona Sikorskaite, and Jari P.T. Valkonen

5. HandGun-Mediated Inoculation of Plants with Viral Pathogens for Mechanistic Studies Victor Gaba, Moshe Lapidot, and Amit Gal-On

6. Biolistics-Based Gene Silencing in Plants Using a Modified Particle Inflow Gun Kevin M. Davies, Simon C. Deroles, Murray R. Boase, Don A. Hunter, and Kathy E. Schwinn

Part II: Biolistic DNA Delivery in Nematodes

7. Biolistic Transformation of Caenorhabditis elegans Meltem Isik and Eugene Berezikov

8. Improved Vectors for Selection of Transgenic Caenorhabditis elegans Annabel A. Ferguson, Liquan Cai, Luv Kashyap, and Alfred L. Fisher

9. Biolistic Transformation of Brugia malayi Tarig B. Higazi and Thomas R. Unnasch

Part III: Biolistic DNA Delivery in Mammalian Cells In Vitro

10. Biolistic Transfection of Human Embryonic Kidney (HEK) 293 Cells Xiongwei Li, Masaki Uchida, H. Oya Alpar, and Peter Mertens

11. Biolistic Transfection of Tumor Tissue Samples Kandan Aravindaram, Shu-Yi Yin, and Ning-Sun Yang

12. Biolistic Transfection of Freshly Isolated Adult Ventricular Myocytes David F. Steele, Ying Dou, and David Fedida

13. Biolistic Transfection of Neurons in Organotypic Brain Slices John A. O’Brien and Sarah C.R. Lummis

Part IV: Biolistic DNA Delivery in Mammalian Cells In Vivo
14. Biolistic DNA Delivery to Mice with the Low Pressure Gene Gun Meng-Chi Yen and Ming-Derg Lai

15. Chemokine Overexpression in the Skin by Biolistic DNA Delivery Ahmad Jalili

16. Enhancement of Gene Gun-Induced Vaccine-Specific Cytotoxic T Cell Response by Administration of Chemotherapeutic Drugs Steve Pascolo

17. Dendritic Cell-Specific Biolistic Transfection Using the Fascin Gene Promoter Yvonne Höhn, Stephan Sudowe, and Angelika B. Reske-Kunz

18. Particle-Mediated Administration of Plasmid DNA on Corneas of BALB/c Mice Dirk Bauer, Susanne Wasmuth, Mengji Lu, and Arnd Heiligenhaus

Part V: Biolistic DNA Vaccination in Animal Disease Models

19. Optimizing Particle-Mediated Epidermal Delivery of an Influenza DNA Vaccine in Ferrets Eric J. Yager, Cristy Stagnar, Ragisha Gopalakrishnan, James T. Fuller, and Deborah H. Fuller

20. Methods for Monitoring Gene Gun-Induced HBV- and HCV-Specific Immune Responses in Mouse Models Gustaf Ahlén, Matti Sällberg, and Lars Frelin

21. Gene Gun Immunization to Combat Malaria Elke S. Bergmann-Leitner and Wolfgang W. Leitner

22. Identification of T-cell Epitopes of Mycobacterium tuberculosis with Biolistic DNA Vaccination Toshi Nagata and Yukio Koide

23. Biolistic DNA Vaccination Against Trypanosoma Infection Marianne Bryan, Siobhan Guyach, and Karen A. Norris

24. Biolistic DNA Vaccination Against Melanoma Julia Steitz and Thomas Tüting

25. Biolistic DNA Vaccination Against Cervical Cancer Michal Šmahel

26. Efficiency of Biolistic DNA Vaccination in Experimental Type I Allergy Verena Raker, Joachim Maxeiner, Angelika B. Reske-Kunz, and Stephan Sudowe

27. Safety Assessment of Biolistic DNA Vaccination Barbara Langer, Matthias Renner, Jürgen Scherer, Silke Schüle, and Klaus Cichutek

Part VI: Related Applications for Biolistic Delivery of Molecules

28. DiOlistics: Delivery of Fluorescent Dyes into Cells Nyssa Sherazee and Veronica A. Alvarez

29. Protein Antigen Delivery by Gene Gun-Mediated Epidermal Antigen Incorporation (EAI) Sandra Scheiblhofer, Uwe Ritter, Josef Thalhamer, and Richard Weiss

Anssi L. Vuorinen, Arto Nieminen, Victor Gaba, Sidona Sikorskaite, and Jari P.T. Valkonen

5. HandGun-Mediated Inoculation of Plants with Viral Pathogens for Mechanistic Studies Victor Gaba, Moshe Lapidot, and Amit Gal-On

6. Biolistics-Based Gene Silencing in Plants Using a Modified Particle Inflow Gun Kevin M. Davies, Simon C. Deroles, Murray R. Boase, Don A. Hunter, and Kathy E. Schwinn

Part II: Biolistic DNA Delivery in Nematodes

7. Biolistic Transformation of Caenorhabditis elegans Meltem Isik and Eugene Berezikov

8. Improved Vectors for Selection of Transgenic Caenorhabditis elegans Annabel A. Ferguson, Liquan Cai, Luv Kashyap, and Alfred L. Fisher

9. Biolistic Transformation of Brugia malayi Tarig B. Higazi and Thomas R. Unnasch

Part III: Biolistic DNA Delivery in Mammalian Cells In Vitro

10. Biolistic Transfection of Human Embryonic Kidney (HEK) 293 Cells Xiongwei Li, Masaki Uchida, H. Oya Alpar, and Peter Mertens

11. Biolistic Transfection of Tumor Tissue Samples Kandan Aravindaram, Shu-Yi Yin, and Ning-Sun Yang

12. Biolistic Transfection of Freshly Isolated Adult Ventricular Myocytes David F. Steele, Ying Dou, and David Fedida

13. Biolistic Transfection of Neurons in Organotypic Brain Slices John A. O’Brien and Sarah C.R. Lummis

Part IV: Biolistic DNA Delivery in Mammalian Cells In Vivo
14. Biolistic DNA Delivery to Mice with the Low Pressure Gene Gun Meng-Chi Yen and Ming-Derg Lai

15. Chemokine Overexpression in the Skin by Biolistic DNA Delivery Ahmad Jalili

16. Enhancement of Gene Gun-Induced Vaccine-Specific Cytotoxic T Cell Response by Administration of Chemotherapeutic Drugs Steve Pascolo

17. Dendritic Cell-Specific Biolistic Transfection Using the Fascin Gene Promoter Yvonne Höhn, Stephan Sudowe, and Angelika B. Reske-Kunz

18. Particle-Mediated Administration of Plasmid DNA on Corneas of BALB/c Mice Dirk Bauer, Susanne Wasmuth, Mengji Lu, and Arnd Heiligenhaus

Part V: Biolistic DNA Vaccination in Animal Disease Models

19. Optimizing Particle-Mediated Epidermal Delivery of an Influenza DNA Vaccine in Ferrets Eric J. Yager, Cristy Stagnar, Ragisha Gopalakrishnan, James T. Fuller, and Deborah H. Fuller

20. Methods for Monitoring Gene Gun-Induced HBV- and HCV-Specific Immune Responses in Mouse Models Gustaf Ahlén, Matti Sällberg, and Lars Frelin

21. Gene Gun Immunization to Combat Malaria Elke S. Bergmann-Leitner and Wolfgang W. Leitner

22. Identification of T-cell Epitopes of Mycobacterium tuberculosis with Biolistic DNA Vaccination Toshi Nagata and Yukio Koide

23. Biolistic DNA Vaccination Against Trypanosoma Infection Marianne Bryan, Siobhan Guyach, and Karen A. Norris

24. Biolistic DNA Vaccination Against Melanoma Julia Steitz and Thomas Tüting

25. Biolistic DNA Vaccination Against Cervical Cancer Michal Šmahel

26. Efficiency of Biolistic DNA Vaccination in Experimental Type I Allergy Verena Raker, Joachim Maxeiner, Angelika B. Reske-Kunz, and Stephan Sudowe

27. Safety Assessment of Biolistic DNA Vaccination Barbara Langer, Matthias Renner, Jürgen Scherer, Silke Schüle, and Klaus Cichutek

Part VI: Related Applications for Biolistic Delivery of Molecules

28. DiOlistics: Delivery of Fluorescent Dyes into Cells Nyssa Sherazee and Veronica A. Alvarez

29. Protein Antigen Delivery by Gene Gun-Mediated Epidermal Antigen Incorporation (EAI) Sandra Scheiblhofer, Uwe Ritter, Josef Thalhamer, and Richard Weiss

Victor Gaba, Moshe Lapidot, and Amit Gal-On

6. Biolistics-Based Gene Silencing in Plants Using a Modified Particle Inflow Gun Kevin M. Davies, Simon C. Deroles, Murray R. Boase, Don A. Hunter, and Kathy E. Schwinn

Part II: Biolistic DNA Delivery in Nematodes

7. Biolistic Transformation of Caenorhabditis elegans Meltem Isik and Eugene Berezikov

8. Improved Vectors for Selection of Transgenic Caenorhabditis elegans Annabel A. Ferguson, Liquan Cai, Luv Kashyap, and Alfred L. Fisher

9. Biolistic Transformation of Brugia malayi Tarig B. Higazi and Thomas R. Unnasch

Part III: Biolistic DNA Delivery in Mammalian Cells In Vitro

10. Biolistic Transfection of Human Embryonic Kidney (HEK) 293 Cells Xiongwei Li, Masaki Uchida, H. Oya Alpar, and Peter Mertens

11. Biolistic Transfection of Tumor Tissue Samples Kandan Aravindaram, Shu-Yi Yin, and Ning-Sun Yang

12. Biolistic Transfection of Freshly Isolated Adult Ventricular Myocytes David F. Steele, Ying Dou, and David Fedida

13. Biolistic Transfection of Neurons in Organotypic Brain Slices John A. O’Brien and Sarah C.R. Lummis

Part IV: Biolistic DNA Delivery in Mammalian Cells In Vivo
14. Biolistic DNA Delivery to Mice with the Low Pressure Gene Gun Meng-Chi Yen and Ming-Derg Lai

15. Chemokine Overexpression in the Skin by Biolistic DNA Delivery Ahmad Jalili

16. Enhancement of Gene Gun-Induced Vaccine-Specific Cytotoxic T Cell Response by Administration of Chemotherapeutic Drugs Steve Pascolo

17. Dendritic Cell-Specific Biolistic Transfection Using the Fascin Gene Promoter Yvonne Höhn, Stephan Sudowe, and Angelika B. Reske-Kunz

18. Particle-Mediated Administration of Plasmid DNA on Corneas of BALB/c Mice Dirk Bauer, Susanne Wasmuth, Mengji Lu, and Arnd Heiligenhaus

Part V: Biolistic DNA Vaccination in Animal Disease Models

19. Optimizing Particle-Mediated Epidermal Delivery of an Influenza DNA Vaccine in Ferrets Eric J. Yager, Cristy Stagnar, Ragisha Gopalakrishnan, James T. Fuller, and Deborah H. Fuller

20. Methods for Monitoring Gene Gun-Induced HBV- and HCV-Specific Immune Responses in Mouse Models Gustaf Ahlén, Matti Sällberg, and Lars Frelin

21. Gene Gun Immunization to Combat Malaria Elke S. Bergmann-Leitner and Wolfgang W. Leitner

22. Identification of T-cell Epitopes of Mycobacterium tuberculosis with Biolistic DNA Vaccination Toshi Nagata and Yukio Koide

23. Biolistic DNA Vaccination Against Trypanosoma Infection Marianne Bryan, Siobhan Guyach, and Karen A. Norris

24. Biolistic DNA Vaccination Against Melanoma Julia Steitz and Thomas Tüting

25. Biolistic DNA Vaccination Against Cervical Cancer Michal Šmahel

26. Efficiency of Biolistic DNA Vaccination in Experimental Type I Allergy Verena Raker, Joachim Maxeiner, Angelika B. Reske-Kunz, and Stephan Sudowe

27. Safety Assessment of Biolistic DNA Vaccination Barbara Langer, Matthias Renner, Jürgen Scherer, Silke Schüle, and Klaus Cichutek

Part VI: Related Applications for Biolistic Delivery of Molecules

28. DiOlistics: Delivery of Fluorescent Dyes into Cells Nyssa Sherazee and Veronica A. Alvarez

29. Protein Antigen Delivery by Gene Gun-Mediated Epidermal Antigen Incorporation (EAI) Sandra Scheiblhofer, Uwe Ritter, Josef Thalhamer, and Richard Weiss

Kevin M. Davies, Simon C. Deroles, Murray R. Boase, Don A. Hunter, and Kathy E. Schwinn

Part II: Biolistic DNA Delivery in Nematodes

7. Biolistic Transformation of Caenorhabditis elegans Meltem Isik and Eugene Berezikov

8. Improved Vectors for Selection of Transgenic Caenorhabditis elegans Annabel A. Ferguson, Liquan Cai, Luv Kashyap, and Alfred L. Fisher

9. Biolistic Transformation of Brugia malayi Tarig B. Higazi and Thomas R. Unnasch

Part III: Biolistic DNA Delivery in Mammalian Cells In Vitro

10. Biolistic Transfection of Human Embryonic Kidney (HEK) 293 Cells Xiongwei Li, Masaki Uchida, H. Oya Alpar, and Peter Mertens

11. Biolistic Transfection of Tumor Tissue Samples Kandan Aravindaram, Shu-Yi Yin, and Ning-Sun Yang

12. Biolistic Transfection of Freshly Isolated Adult Ventricular Myocytes David F. Steele, Ying Dou, and David Fedida

13. Biolistic Transfection of Neurons in Organotypic Brain Slices John A. O’Brien and Sarah C.R. Lummis

Part IV: Biolistic DNA Delivery in Mammalian Cells In Vivo
14. Biolistic DNA Delivery to Mice with the Low Pressure Gene Gun Meng-Chi Yen and Ming-Derg Lai

15. Chemokine Overexpression in the Skin by Biolistic DNA Delivery Ahmad Jalili

16. Enhancement of Gene Gun-Induced Vaccine-Specific Cytotoxic T Cell Response by Administration of Chemotherapeutic Drugs Steve Pascolo

17. Dendritic Cell-Specific Biolistic Transfection Using the Fascin Gene Promoter Yvonne Höhn, Stephan Sudowe, and Angelika B. Reske-Kunz

18. Particle-Mediated Administration of Plasmid DNA on Corneas of BALB/c Mice Dirk Bauer, Susanne Wasmuth, Mengji Lu, and Arnd Heiligenhaus

Part V: Biolistic DNA Vaccination in Animal Disease Models

19. Optimizing Particle-Mediated Epidermal Delivery of an Influenza DNA Vaccine in Ferrets Eric J. Yager, Cristy Stagnar, Ragisha Gopalakrishnan, James T. Fuller, and Deborah H. Fuller

20. Methods for Monitoring Gene Gun-Induced HBV- and HCV-Specific Immune Responses in Mouse Models Gustaf Ahlén, Matti Sällberg, and Lars Frelin

21. Gene Gun Immunization to Combat Malaria Elke S. Bergmann-Leitner and Wolfgang W. Leitner

22. Identification of T-cell Epitopes of Mycobacterium tuberculosis with Biolistic DNA Vaccination Toshi Nagata and Yukio Koide

23. Biolistic DNA Vaccination Against Trypanosoma Infection Marianne Bryan, Siobhan Guyach, and Karen A. Norris

24. Biolistic DNA Vaccination Against Melanoma Julia Steitz and Thomas Tüting

25. Biolistic DNA Vaccination Against Cervical Cancer Michal Šmahel

26. Efficiency of Biolistic DNA Vaccination in Experimental Type I Allergy Verena Raker, Joachim Maxeiner, Angelika B. Reske-Kunz, and Stephan Sudowe

27. Safety Assessment of Biolistic DNA Vaccination Barbara Langer, Matthias Renner, Jürgen Scherer, Silke Schüle, and Klaus Cichutek

Part VI: Related Applications for Biolistic Delivery of Molecules

28. DiOlistics: Delivery of Fluorescent Dyes into Cells Nyssa Sherazee and Veronica A. Alvarez

29. Protein Antigen Delivery by Gene Gun-Mediated Epidermal Antigen Incorporation (EAI) Sandra Scheiblhofer, Uwe Ritter, Josef Thalhamer, and Richard Weiss

Meltem Isik and Eugene Berezikov

8. Improved Vectors for Selection of Transgenic Caenorhabditis elegans Annabel A. Ferguson, Liquan Cai, Luv Kashyap, and Alfred L. Fisher

9. Biolistic Transformation of Brugia malayi Tarig B. Higazi and Thomas R. Unnasch

Part III: Biolistic DNA Delivery in Mammalian Cells In Vitro

10. Biolistic Transfection of Human Embryonic Kidney (HEK) 293 Cells Xiongwei Li, Masaki Uchida, H. Oya Alpar, and Peter Mertens

11. Biolistic Transfection of Tumor Tissue Samples Kandan Aravindaram, Shu-Yi Yin, and Ning-Sun Yang

12. Biolistic Transfection of Freshly Isolated Adult Ventricular Myocytes David F. Steele, Ying Dou, and David Fedida

13. Biolistic Transfection of Neurons in Organotypic Brain Slices John A. O’Brien and Sarah C.R. Lummis

Part IV: Biolistic DNA Delivery in Mammalian Cells In Vivo
14. Biolistic DNA Delivery to Mice with the Low Pressure Gene Gun Meng-Chi Yen and Ming-Derg Lai

15. Chemokine Overexpression in the Skin by Biolistic DNA Delivery Ahmad Jalili

16. Enhancement of Gene Gun-Induced Vaccine-Specific Cytotoxic T Cell Response by Administration of Chemotherapeutic Drugs Steve Pascolo

17. Dendritic Cell-Specific Biolistic Transfection Using the Fascin Gene Promoter Yvonne Höhn, Stephan Sudowe, and Angelika B. Reske-Kunz

18. Particle-Mediated Administration of Plasmid DNA on Corneas of BALB/c Mice Dirk Bauer, Susanne Wasmuth, Mengji Lu, and Arnd Heiligenhaus

Part V: Biolistic DNA Vaccination in Animal Disease Models

19. Optimizing Particle-Mediated Epidermal Delivery of an Influenza DNA Vaccine in Ferrets Eric J. Yager, Cristy Stagnar, Ragisha Gopalakrishnan, James T. Fuller, and Deborah H. Fuller

20. Methods for Monitoring Gene Gun-Induced HBV- and HCV-Specific Immune Responses in Mouse Models Gustaf Ahlén, Matti Sällberg, and Lars Frelin

21. Gene Gun Immunization to Combat Malaria Elke S. Bergmann-Leitner and Wolfgang W. Leitner

22. Identification of T-cell Epitopes of Mycobacterium tuberculosis with Biolistic DNA Vaccination Toshi Nagata and Yukio Koide

23. Biolistic DNA Vaccination Against Trypanosoma Infection Marianne Bryan, Siobhan Guyach, and Karen A. Norris

24. Biolistic DNA Vaccination Against Melanoma Julia Steitz and Thomas Tüting

25. Biolistic DNA Vaccination Against Cervical Cancer Michal Šmahel

26. Efficiency of Biolistic DNA Vaccination in Experimental Type I Allergy Verena Raker, Joachim Maxeiner, Angelika B. Reske-Kunz, and Stephan Sudowe

27. Safety Assessment of Biolistic DNA Vaccination Barbara Langer, Matthias Renner, Jürgen Scherer, Silke Schüle, and Klaus Cichutek

Part VI: Related Applications for Biolistic Delivery of Molecules

28. DiOlistics: Delivery of Fluorescent Dyes into Cells Nyssa Sherazee and Veronica A. Alvarez

29. Protein Antigen Delivery by Gene Gun-Mediated Epidermal Antigen Incorporation (EAI) Sandra Scheiblhofer, Uwe Ritter, Josef Thalhamer, and Richard Weiss

Annabel A. Ferguson, Liquan Cai, Luv Kashyap, and Alfred L. Fisher

9. Biolistic Transformation of Brugia malayi Tarig B. Higazi and Thomas R. Unnasch

Part III: Biolistic DNA Delivery in Mammalian Cells In Vitro

10. Biolistic Transfection of Human Embryonic Kidney (HEK) 293 Cells Xiongwei Li, Masaki Uchida, H. Oya Alpar, and Peter Mertens

11. Biolistic Transfection of Tumor Tissue Samples Kandan Aravindaram, Shu-Yi Yin, and Ning-Sun Yang

12. Biolistic Transfection of Freshly Isolated Adult Ventricular Myocytes David F. Steele, Ying Dou, and David Fedida

13. Biolistic Transfection of Neurons in Organotypic Brain Slices John A. O’Brien and Sarah C.R. Lummis

Part IV: Biolistic DNA Delivery in Mammalian Cells In Vivo
14. Biolistic DNA Delivery to Mice with the Low Pressure Gene Gun Meng-Chi Yen and Ming-Derg Lai

15. Chemokine Overexpression in the Skin by Biolistic DNA Delivery Ahmad Jalili

16. Enhancement of Gene Gun-Induced Vaccine-Specific Cytotoxic T Cell Response by Administration of Chemotherapeutic Drugs Steve Pascolo

17. Dendritic Cell-Specific Biolistic Transfection Using the Fascin Gene Promoter Yvonne Höhn, Stephan Sudowe, and Angelika B. Reske-Kunz

18. Particle-Mediated Administration of Plasmid DNA on Corneas of BALB/c Mice Dirk Bauer, Susanne Wasmuth, Mengji Lu, and Arnd Heiligenhaus

Part V: Biolistic DNA Vaccination in Animal Disease Models

19. Optimizing Particle-Mediated Epidermal Delivery of an Influenza DNA Vaccine in Ferrets Eric J. Yager, Cristy Stagnar, Ragisha Gopalakrishnan, James T. Fuller, and Deborah H. Fuller

20. Methods for Monitoring Gene Gun-Induced HBV- and HCV-Specific Immune Responses in Mouse Models Gustaf Ahlén, Matti Sällberg, and Lars Frelin

21. Gene Gun Immunization to Combat Malaria Elke S. Bergmann-Leitner and Wolfgang W. Leitner

22. Identification of T-cell Epitopes of Mycobacterium tuberculosis with Biolistic DNA Vaccination Toshi Nagata and Yukio Koide

23. Biolistic DNA Vaccination Against Trypanosoma Infection Marianne Bryan, Siobhan Guyach, and Karen A. Norris

24. Biolistic DNA Vaccination Against Melanoma Julia Steitz and Thomas Tüting

25. Biolistic DNA Vaccination Against Cervical Cancer Michal Šmahel

26. Efficiency of Biolistic DNA Vaccination in Experimental Type I Allergy Verena Raker, Joachim Maxeiner, Angelika B. Reske-Kunz, and Stephan Sudowe

27. Safety Assessment of Biolistic DNA Vaccination Barbara Langer, Matthias Renner, Jürgen Scherer, Silke Schüle, and Klaus Cichutek

Part VI: Related Applications for Biolistic Delivery of Molecules

28. DiOlistics: Delivery of Fluorescent Dyes into Cells Nyssa Sherazee and Veronica A. Alvarez

29. Protein Antigen Delivery by Gene Gun-Mediated Epidermal Antigen Incorporation (EAI) Sandra Scheiblhofer, Uwe Ritter, Josef Thalhamer, and Richard Weiss

Tarig B. Higazi and Thomas R. Unnasch

Part III: Biolistic DNA Delivery in Mammalian Cells In Vitro

10. Biolistic Transfection of Human Embryonic Kidney (HEK) 293 Cells Xiongwei Li, Masaki Uchida, H. Oya Alpar, and Peter Mertens

11. Biolistic Transfection of Tumor Tissue Samples Kandan Aravindaram, Shu-Yi Yin, and Ning-Sun Yang

12. Biolistic Transfection of Freshly Isolated Adult Ventricular Myocytes David F. Steele, Ying Dou, and David Fedida

13. Biolistic Transfection of Neurons in Organotypic Brain Slices John A. O’Brien and Sarah C.R. Lummis

Part IV: Biolistic DNA Delivery in Mammalian Cells In Vivo
14. Biolistic DNA Delivery to Mice with the Low Pressure Gene Gun Meng-Chi Yen and Ming-Derg Lai

15. Chemokine Overexpression in the Skin by Biolistic DNA Delivery Ahmad Jalili

16. Enhancement of Gene Gun-Induced Vaccine-Specific Cytotoxic T Cell Response by Administration of Chemotherapeutic Drugs Steve Pascolo

17. Dendritic Cell-Specific Biolistic Transfection Using the Fascin Gene Promoter Yvonne Höhn, Stephan Sudowe, and Angelika B. Reske-Kunz

18. Particle-Mediated Administration of Plasmid DNA on Corneas of BALB/c Mice Dirk Bauer, Susanne Wasmuth, Mengji Lu, and Arnd Heiligenhaus

Part V: Biolistic DNA Vaccination in Animal Disease Models

19. Optimizing Particle-Mediated Epidermal Delivery of an Influenza DNA Vaccine in Ferrets Eric J. Yager, Cristy Stagnar, Ragisha Gopalakrishnan, James T. Fuller, and Deborah H. Fuller

20. Methods for Monitoring Gene Gun-Induced HBV- and HCV-Specific Immune Responses in Mouse Models Gustaf Ahlén, Matti Sällberg, and Lars Frelin

21. Gene Gun Immunization to Combat Malaria Elke S. Bergmann-Leitner and Wolfgang W. Leitner

22. Identification of T-cell Epitopes of Mycobacterium tuberculosis with Biolistic DNA Vaccination Toshi Nagata and Yukio Koide

23. Biolistic DNA Vaccination Against Trypanosoma Infection Marianne Bryan, Siobhan Guyach, and Karen A. Norris

24. Biolistic DNA Vaccination Against Melanoma Julia Steitz and Thomas Tüting

25. Biolistic DNA Vaccination Against Cervical Cancer Michal Šmahel

26. Efficiency of Biolistic DNA Vaccination in Experimental Type I Allergy Verena Raker, Joachim Maxeiner, Angelika B. Reske-Kunz, and Stephan Sudowe

27. Safety Assessment of Biolistic DNA Vaccination Barbara Langer, Matthias Renner, Jürgen Scherer, Silke Schüle, and Klaus Cichutek

Part VI: Related Applications for Biolistic Delivery of Molecules

28. DiOlistics: Delivery of Fluorescent Dyes into Cells Nyssa Sherazee and Veronica A. Alvarez

29. Protein Antigen Delivery by Gene Gun-Mediated Epidermal Antigen Incorporation (EAI) Sandra Scheiblhofer, Uwe Ritter, Josef Thalhamer, and Richard Weiss

Xiongwei Li, Masaki Uchida, H. Oya Alpar, and Peter Mertens

11. Biolistic Transfection of Tumor Tissue Samples Kandan Aravindaram, Shu-Yi Yin, and Ning-Sun Yang

12. Biolistic Transfection of Freshly Isolated Adult Ventricular Myocytes David F. Steele, Ying Dou, and David Fedida

13. Biolistic Transfection of Neurons in Organotypic Brain Slices John A. O’Brien and Sarah C.R. Lummis

Part IV: Biolistic DNA Delivery in Mammalian Cells In Vivo
14. Biolistic DNA Delivery to Mice with the Low Pressure Gene Gun Meng-Chi Yen and Ming-Derg Lai

15. Chemokine Overexpression in the Skin by Biolistic DNA Delivery Ahmad Jalili

16. Enhancement of Gene Gun-Induced Vaccine-Specific Cytotoxic T Cell Response by Administration of Chemotherapeutic Drugs Steve Pascolo

17. Dendritic Cell-Specific Biolistic Transfection Using the Fascin Gene Promoter Yvonne Höhn, Stephan Sudowe, and Angelika B. Reske-Kunz

18. Particle-Mediated Administration of Plasmid DNA on Corneas of BALB/c Mice Dirk Bauer, Susanne Wasmuth, Mengji Lu, and Arnd Heiligenhaus

Part V: Biolistic DNA Vaccination in Animal Disease Models

19. Optimizing Particle-Mediated Epidermal Delivery of an Influenza DNA Vaccine in Ferrets Eric J. Yager, Cristy Stagnar, Ragisha Gopalakrishnan, James T. Fuller, and Deborah H. Fuller

20. Methods for Monitoring Gene Gun-Induced HBV- and HCV-Specific Immune Responses in Mouse Models Gustaf Ahlén, Matti Sällberg, and Lars Frelin

21. Gene Gun Immunization to Combat Malaria Elke S. Bergmann-Leitner and Wolfgang W. Leitner

22. Identification of T-cell Epitopes of Mycobacterium tuberculosis with Biolistic DNA Vaccination Toshi Nagata and Yukio Koide

23. Biolistic DNA Vaccination Against Trypanosoma Infection Marianne Bryan, Siobhan Guyach, and Karen A. Norris

24. Biolistic DNA Vaccination Against Melanoma Julia Steitz and Thomas Tüting

25. Biolistic DNA Vaccination Against Cervical Cancer Michal Šmahel

26. Efficiency of Biolistic DNA Vaccination in Experimental Type I Allergy Verena Raker, Joachim Maxeiner, Angelika B. Reske-Kunz, and Stephan Sudowe

27. Safety Assessment of Biolistic DNA Vaccination Barbara Langer, Matthias Renner, Jürgen Scherer, Silke Schüle, and Klaus Cichutek

Part VI: Related Applications for Biolistic Delivery of Molecules

28. DiOlistics: Delivery of Fluorescent Dyes into Cells Nyssa Sherazee and Veronica A. Alvarez

29. Protein Antigen Delivery by Gene Gun-Mediated Epidermal Antigen Incorporation (EAI) Sandra Scheiblhofer, Uwe Ritter, Josef Thalhamer, and Richard Weiss

Kandan Aravindaram, Shu-Yi Yin, and Ning-Sun Yang

12. Biolistic Transfection of Freshly Isolated Adult Ventricular Myocytes David F. Steele, Ying Dou, and David Fedida

13. Biolistic Transfection of Neurons in Organotypic Brain Slices John A. O’Brien and Sarah C.R. Lummis

Part IV: Biolistic DNA Delivery in Mammalian Cells In Vivo
14. Biolistic DNA Delivery to Mice with the Low Pressure Gene Gun Meng-Chi Yen and Ming-Derg Lai

15. Chemokine Overexpression in the Skin by Biolistic DNA Delivery Ahmad Jalili

16. Enhancement of Gene Gun-Induced Vaccine-Specific Cytotoxic T Cell Response by Administration of Chemotherapeutic Drugs Steve Pascolo

17. Dendritic Cell-Specific Biolistic Transfection Using the Fascin Gene Promoter Yvonne Höhn, Stephan Sudowe, and Angelika B. Reske-Kunz

18. Particle-Mediated Administration of Plasmid DNA on Corneas of BALB/c Mice Dirk Bauer, Susanne Wasmuth, Mengji Lu, and Arnd Heiligenhaus

Part V: Biolistic DNA Vaccination in Animal Disease Models

19. Optimizing Particle-Mediated Epidermal Delivery of an Influenza DNA Vaccine in Ferrets Eric J. Yager, Cristy Stagnar, Ragisha Gopalakrishnan, James T. Fuller, and Deborah H. Fuller

20. Methods for Monitoring Gene Gun-Induced HBV- and HCV-Specific Immune Responses in Mouse Models Gustaf Ahlén, Matti Sällberg, and Lars Frelin

21. Gene Gun Immunization to Combat Malaria Elke S. Bergmann-Leitner and Wolfgang W. Leitner

22. Identification of T-cell Epitopes of Mycobacterium tuberculosis with Biolistic DNA Vaccination Toshi Nagata and Yukio Koide

23. Biolistic DNA Vaccination Against Trypanosoma Infection Marianne Bryan, Siobhan Guyach, and Karen A. Norris

24. Biolistic DNA Vaccination Against Melanoma Julia Steitz and Thomas Tüting

25. Biolistic DNA Vaccination Against Cervical Cancer Michal Šmahel

26. Efficiency of Biolistic DNA Vaccination in Experimental Type I Allergy Verena Raker, Joachim Maxeiner, Angelika B. Reske-Kunz, and Stephan Sudowe

27. Safety Assessment of Biolistic DNA Vaccination Barbara Langer, Matthias Renner, Jürgen Scherer, Silke Schüle, and Klaus Cichutek

Part VI: Related Applications for Biolistic Delivery of Molecules

28. DiOlistics: Delivery of Fluorescent Dyes into Cells Nyssa Sherazee and Veronica A. Alvarez

29. Protein Antigen Delivery by Gene Gun-Mediated Epidermal Antigen Incorporation (EAI) Sandra Scheiblhofer, Uwe Ritter, Josef Thalhamer, and Richard Weiss

David F. Steele, Ying Dou, and David Fedida

13. Biolistic Transfection of Neurons in Organotypic Brain Slices John A. O’Brien and Sarah C.R. Lummis

Part IV: Biolistic DNA Delivery in Mammalian Cells In Vivo
14. Biolistic DNA Delivery to Mice with the Low Pressure Gene Gun Meng-Chi Yen and Ming-Derg Lai

15. Chemokine Overexpression in the Skin by Biolistic DNA Delivery Ahmad Jalili

16. Enhancement of Gene Gun-Induced Vaccine-Specific Cytotoxic T Cell Response by Administration of Chemotherapeutic Drugs Steve Pascolo

17. Dendritic Cell-Specific Biolistic Transfection Using the Fascin Gene Promoter Yvonne Höhn, Stephan Sudowe, and Angelika B. Reske-Kunz

18. Particle-Mediated Administration of Plasmid DNA on Corneas of BALB/c Mice Dirk Bauer, Susanne Wasmuth, Mengji Lu, and Arnd Heiligenhaus

Part V: Biolistic DNA Vaccination in Animal Disease Models

19. Optimizing Particle-Mediated Epidermal Delivery of an Influenza DNA Vaccine in Ferrets Eric J. Yager, Cristy Stagnar, Ragisha Gopalakrishnan, James T. Fuller, and Deborah H. Fuller

20. Methods for Monitoring Gene Gun-Induced HBV- and HCV-Specific Immune Responses in Mouse Models Gustaf Ahlén, Matti Sällberg, and Lars Frelin

21. Gene Gun Immunization to Combat Malaria Elke S. Bergmann-Leitner and Wolfgang W. Leitner

22. Identification of T-cell Epitopes of Mycobacterium tuberculosis with Biolistic DNA Vaccination Toshi Nagata and Yukio Koide

23. Biolistic DNA Vaccination Against Trypanosoma Infection Marianne Bryan, Siobhan Guyach, and Karen A. Norris

24. Biolistic DNA Vaccination Against Melanoma Julia Steitz and Thomas Tüting

25. Biolistic DNA Vaccination Against Cervical Cancer Michal Šmahel

26. Efficiency of Biolistic DNA Vaccination in Experimental Type I Allergy Verena Raker, Joachim Maxeiner, Angelika B. Reske-Kunz, and Stephan Sudowe

27. Safety Assessment of Biolistic DNA Vaccination Barbara Langer, Matthias Renner, Jürgen Scherer, Silke Schüle, and Klaus Cichutek

Part VI: Related Applications for Biolistic Delivery of Molecules

28. DiOlistics: Delivery of Fluorescent Dyes into Cells Nyssa Sherazee and Veronica A. Alvarez

29. Protein Antigen Delivery by Gene Gun-Mediated Epidermal Antigen Incorporation (EAI) Sandra Scheiblhofer, Uwe Ritter, Josef Thalhamer, and Richard Weiss

John A. O’Brien and Sarah C.R. Lummis

Part IV: Biolistic DNA Delivery in Mammalian Cells In Vivo
14. Biolistic DNA Delivery to Mice with the Low Pressure Gene Gun Meng-Chi Yen and Ming-Derg Lai

15. Chemokine Overexpression in the Skin by Biolistic DNA Delivery Ahmad Jalili

16. Enhancement of Gene Gun-Induced Vaccine-Specific Cytotoxic T Cell Response by Administration of Chemotherapeutic Drugs Steve Pascolo

17. Dendritic Cell-Specific Biolistic Transfection Using the Fascin Gene Promoter Yvonne Höhn, Stephan Sudowe, and Angelika B. Reske-Kunz

18. Particle-Mediated Administration of Plasmid DNA on Corneas of BALB/c Mice Dirk Bauer, Susanne Wasmuth, Mengji Lu, and Arnd Heiligenhaus

Part V: Biolistic DNA Vaccination in Animal Disease Models

19. Optimizing Particle-Mediated Epidermal Delivery of an Influenza DNA Vaccine in Ferrets Eric J. Yager, Cristy Stagnar, Ragisha Gopalakrishnan, James T. Fuller, and Deborah H. Fuller

20. Methods for Monitoring Gene Gun-Induced HBV- and HCV-Specific Immune Responses in Mouse Models Gustaf Ahlén, Matti Sällberg, and Lars Frelin

21. Gene Gun Immunization to Combat Malaria Elke S. Bergmann-Leitner and Wolfgang W. Leitner

22. Identification of T-cell Epitopes of Mycobacterium tuberculosis with Biolistic DNA Vaccination Toshi Nagata and Yukio Koide

23. Biolistic DNA Vaccination Against Trypanosoma Infection Marianne Bryan, Siobhan Guyach, and Karen A. Norris

24. Biolistic DNA Vaccination Against Melanoma Julia Steitz and Thomas Tüting

25. Biolistic DNA Vaccination Against Cervical Cancer Michal Šmahel

26. Efficiency of Biolistic DNA Vaccination in Experimental Type I Allergy Verena Raker, Joachim Maxeiner, Angelika B. Reske-Kunz, and Stephan Sudowe

27. Safety Assessment of Biolistic DNA Vaccination Barbara Langer, Matthias Renner, Jürgen Scherer, Silke Schüle, and Klaus Cichutek

Part VI: Related Applications for Biolistic Delivery of Molecules

28. DiOlistics: Delivery of Fluorescent Dyes into Cells Nyssa Sherazee and Veronica A. Alvarez

29. Protein Antigen Delivery by Gene Gun-Mediated Epidermal Antigen Incorporation (EAI) Sandra Scheiblhofer, Uwe Ritter, Josef Thalhamer, and Richard Weiss

14. Biolistic DNA Delivery to Mice with the Low Pressure Gene Gun Meng-Chi Yen and Ming-Derg Lai

15. Chemokine Overexpression in the Skin by Biolistic DNA Delivery Ahmad Jalili

16. Enhancement of Gene Gun-Induced Vaccine-Specific Cytotoxic T Cell Response by Administration of Chemotherapeutic Drugs Steve Pascolo

17. Dendritic Cell-Specific Biolistic Transfection Using the Fascin Gene Promoter Yvonne Höhn, Stephan Sudowe, and Angelika B. Reske-Kunz

18. Particle-Mediated Administration of Plasmid DNA on Corneas of BALB/c Mice Dirk Bauer, Susanne Wasmuth, Mengji Lu, and Arnd Heiligenhaus

Part V: Biolistic DNA Vaccination in Animal Disease Models

19. Optimizing Particle-Mediated Epidermal Delivery of an Influenza DNA Vaccine in Ferrets Eric J. Yager, Cristy Stagnar, Ragisha Gopalakrishnan, James T. Fuller, and Deborah H. Fuller

20. Methods for Monitoring Gene Gun-Induced HBV- and HCV-Specific Immune Responses in Mouse Models Gustaf Ahlén, Matti Sällberg, and Lars Frelin

21. Gene Gun Immunization to Combat Malaria Elke S. Bergmann-Leitner and Wolfgang W. Leitner

22. Identification of T-cell Epitopes of Mycobacterium tuberculosis with Biolistic DNA Vaccination Toshi Nagata and Yukio Koide

23. Biolistic DNA Vaccination Against Trypanosoma Infection Marianne Bryan, Siobhan Guyach, and Karen A. Norris

24. Biolistic DNA Vaccination Against Melanoma Julia Steitz and Thomas Tüting

25. Biolistic DNA Vaccination Against Cervical Cancer Michal Šmahel

26. Efficiency of Biolistic DNA Vaccination in Experimental Type I Allergy Verena Raker, Joachim Maxeiner, Angelika B. Reske-Kunz, and Stephan Sudowe

27. Safety Assessment of Biolistic DNA Vaccination Barbara Langer, Matthias Renner, Jürgen Scherer, Silke Schüle, and Klaus Cichutek

Part VI: Related Applications for Biolistic Delivery of Molecules

28. DiOlistics: Delivery of Fluorescent Dyes into Cells Nyssa Sherazee and Veronica A. Alvarez

29. Protein Antigen Delivery by Gene Gun-Mediated Epidermal Antigen Incorporation (EAI) Sandra Scheiblhofer, Uwe Ritter, Josef Thalhamer, and Richard Weiss

Meng-Chi Yen and Ming-Derg Lai

15. Chemokine Overexpression in the Skin by Biolistic DNA Delivery Ahmad Jalili

16. Enhancement of Gene Gun-Induced Vaccine-Specific Cytotoxic T Cell Response by Administration of Chemotherapeutic Drugs Steve Pascolo

17. Dendritic Cell-Specific Biolistic Transfection Using the Fascin Gene Promoter Yvonne Höhn, Stephan Sudowe, and Angelika B. Reske-Kunz

18. Particle-Mediated Administration of Plasmid DNA on Corneas of BALB/c Mice Dirk Bauer, Susanne Wasmuth, Mengji Lu, and Arnd Heiligenhaus

Part V: Biolistic DNA Vaccination in Animal Disease Models

19. Optimizing Particle-Mediated Epidermal Delivery of an Influenza DNA Vaccine in Ferrets Eric J. Yager, Cristy Stagnar, Ragisha Gopalakrishnan, James T. Fuller, and Deborah H. Fuller

20. Methods for Monitoring Gene Gun-Induced HBV- and HCV-Specific Immune Responses in Mouse Models Gustaf Ahlén, Matti Sällberg, and Lars Frelin

21. Gene Gun Immunization to Combat Malaria Elke S. Bergmann-Leitner and Wolfgang W. Leitner

22. Identification of T-cell Epitopes of Mycobacterium tuberculosis with Biolistic DNA Vaccination Toshi Nagata and Yukio Koide

23. Biolistic DNA Vaccination Against Trypanosoma Infection Marianne Bryan, Siobhan Guyach, and Karen A. Norris

24. Biolistic DNA Vaccination Against Melanoma Julia Steitz and Thomas Tüting

25. Biolistic DNA Vaccination Against Cervical Cancer Michal Šmahel

26. Efficiency of Biolistic DNA Vaccination in Experimental Type I Allergy Verena Raker, Joachim Maxeiner, Angelika B. Reske-Kunz, and Stephan Sudowe

27. Safety Assessment of Biolistic DNA Vaccination Barbara Langer, Matthias Renner, Jürgen Scherer, Silke Schüle, and Klaus Cichutek

Part VI: Related Applications for Biolistic Delivery of Molecules

28. DiOlistics: Delivery of Fluorescent Dyes into Cells Nyssa Sherazee and Veronica A. Alvarez

29. Protein Antigen Delivery by Gene Gun-Mediated Epidermal Antigen Incorporation (EAI) Sandra Scheiblhofer, Uwe Ritter, Josef Thalhamer, and Richard Weiss

Ahmad Jalili

16. Enhancement of Gene Gun-Induced Vaccine-Specific Cytotoxic T Cell Response by Administration of Chemotherapeutic Drugs Steve Pascolo

17. Dendritic Cell-Specific Biolistic Transfection Using the Fascin Gene Promoter Yvonne Höhn, Stephan Sudowe, and Angelika B. Reske-Kunz

18. Particle-Mediated Administration of Plasmid DNA on Corneas of BALB/c Mice Dirk Bauer, Susanne Wasmuth, Mengji Lu, and Arnd Heiligenhaus

Part V: Biolistic DNA Vaccination in Animal Disease Models

19. Optimizing Particle-Mediated Epidermal Delivery of an Influenza DNA Vaccine in Ferrets Eric J. Yager, Cristy Stagnar, Ragisha Gopalakrishnan, James T. Fuller, and Deborah H. Fuller

20. Methods for Monitoring Gene Gun-Induced HBV- and HCV-Specific Immune Responses in Mouse Models Gustaf Ahlén, Matti Sällberg, and Lars Frelin

21. Gene Gun Immunization to Combat Malaria Elke S. Bergmann-Leitner and Wolfgang W. Leitner

22. Identification of T-cell Epitopes of Mycobacterium tuberculosis with Biolistic DNA Vaccination Toshi Nagata and Yukio Koide

23. Biolistic DNA Vaccination Against Trypanosoma Infection Marianne Bryan, Siobhan Guyach, and Karen A. Norris

24. Biolistic DNA Vaccination Against Melanoma Julia Steitz and Thomas Tüting

25. Biolistic DNA Vaccination Against Cervical Cancer Michal Šmahel

26. Efficiency of Biolistic DNA Vaccination in Experimental Type I Allergy Verena Raker, Joachim Maxeiner, Angelika B. Reske-Kunz, and Stephan Sudowe

27. Safety Assessment of Biolistic DNA Vaccination Barbara Langer, Matthias Renner, Jürgen Scherer, Silke Schüle, and Klaus Cichutek

Part VI: Related Applications for Biolistic Delivery of Molecules

28. DiOlistics: Delivery of Fluorescent Dyes into Cells Nyssa Sherazee and Veronica A. Alvarez

29. Protein Antigen Delivery by Gene Gun-Mediated Epidermal Antigen Incorporation (EAI) Sandra Scheiblhofer, Uwe Ritter, Josef Thalhamer, and Richard Weiss

Steve Pascolo

17. Dendritic Cell-Specific Biolistic Transfection Using the Fascin Gene Promoter Yvonne Höhn, Stephan Sudowe, and Angelika B. Reske-Kunz

18. Particle-Mediated Administration of Plasmid DNA on Corneas of BALB/c Mice Dirk Bauer, Susanne Wasmuth, Mengji Lu, and Arnd Heiligenhaus

Part V: Biolistic DNA Vaccination in Animal Disease Models

19. Optimizing Particle-Mediated Epidermal Delivery of an Influenza DNA Vaccine in Ferrets Eric J. Yager, Cristy Stagnar, Ragisha Gopalakrishnan, James T. Fuller, and Deborah H. Fuller

20. Methods for Monitoring Gene Gun-Induced HBV- and HCV-Specific Immune Responses in Mouse Models Gustaf Ahlén, Matti Sällberg, and Lars Frelin

21. Gene Gun Immunization to Combat Malaria Elke S. Bergmann-Leitner and Wolfgang W. Leitner

22. Identification of T-cell Epitopes of Mycobacterium tuberculosis with Biolistic DNA Vaccination Toshi Nagata and Yukio Koide

23. Biolistic DNA Vaccination Against Trypanosoma Infection Marianne Bryan, Siobhan Guyach, and Karen A. Norris

24. Biolistic DNA Vaccination Against Melanoma Julia Steitz and Thomas Tüting

25. Biolistic DNA Vaccination Against Cervical Cancer Michal Šmahel

26. Efficiency of Biolistic DNA Vaccination in Experimental Type I Allergy Verena Raker, Joachim Maxeiner, Angelika B. Reske-Kunz, and Stephan Sudowe

27. Safety Assessment of Biolistic DNA Vaccination Barbara Langer, Matthias Renner, Jürgen Scherer, Silke Schüle, and Klaus Cichutek

Part VI: Related Applications for Biolistic Delivery of Molecules

28. DiOlistics: Delivery of Fluorescent Dyes into Cells Nyssa Sherazee and Veronica A. Alvarez

29. Protein Antigen Delivery by Gene Gun-Mediated Epidermal Antigen Incorporation (EAI) Sandra Scheiblhofer, Uwe Ritter, Josef Thalhamer, and Richard Weiss

Yvonne Höhn, Stephan Sudowe, and Angelika B. Reske-Kunz

18. Particle-Mediated Administration of Plasmid DNA on Corneas of BALB/c Mice Dirk Bauer, Susanne Wasmuth, Mengji Lu, and Arnd Heiligenhaus

Part V: Biolistic DNA Vaccination in Animal Disease Models

19. Optimizing Particle-Mediated Epidermal Delivery of an Influenza DNA Vaccine in Ferrets Eric J. Yager, Cristy Stagnar, Ragisha Gopalakrishnan, James T. Fuller, and Deborah H. Fuller

20. Methods for Monitoring Gene Gun-Induced HBV- and HCV-Specific Immune Responses in Mouse Models Gustaf Ahlén, Matti Sällberg, and Lars Frelin

21. Gene Gun Immunization to Combat Malaria Elke S. Bergmann-Leitner and Wolfgang W. Leitner

22. Identification of T-cell Epitopes of Mycobacterium tuberculosis with Biolistic DNA Vaccination Toshi Nagata and Yukio Koide

23. Biolistic DNA Vaccination Against Trypanosoma Infection Marianne Bryan, Siobhan Guyach, and Karen A. Norris

24. Biolistic DNA Vaccination Against Melanoma Julia Steitz and Thomas Tüting

25. Biolistic DNA Vaccination Against Cervical Cancer Michal Šmahel

26. Efficiency of Biolistic DNA Vaccination in Experimental Type I Allergy Verena Raker, Joachim Maxeiner, Angelika B. Reske-Kunz, and Stephan Sudowe

27. Safety Assessment of Biolistic DNA Vaccination Barbara Langer, Matthias Renner, Jürgen Scherer, Silke Schüle, and Klaus Cichutek

Part VI: Related Applications for Biolistic Delivery of Molecules

28. DiOlistics: Delivery of Fluorescent Dyes into Cells Nyssa Sherazee and Veronica A. Alvarez

29. Protein Antigen Delivery by Gene Gun-Mediated Epidermal Antigen Incorporation (EAI) Sandra Scheiblhofer, Uwe Ritter, Josef Thalhamer, and Richard Weiss

Dirk Bauer, Susanne Wasmuth, Mengji Lu, and Arnd Heiligenhaus

Part V: Biolistic DNA Vaccination in Animal Disease Models

19. Optimizing Particle-Mediated Epidermal Delivery of an Influenza DNA Vaccine in Ferrets Eric J. Yager, Cristy Stagnar, Ragisha Gopalakrishnan, James T. Fuller, and Deborah H. Fuller

20. Methods for Monitoring Gene Gun-Induced HBV- and HCV-Specific Immune Responses in Mouse Models Gustaf Ahlén, Matti Sällberg, and Lars Frelin

21. Gene Gun Immunization to Combat Malaria Elke S. Bergmann-Leitner and Wolfgang W. Leitner

22. Identification of T-cell Epitopes of Mycobacterium tuberculosis with Biolistic DNA Vaccination Toshi Nagata and Yukio Koide

23. Biolistic DNA Vaccination Against Trypanosoma Infection Marianne Bryan, Siobhan Guyach, and Karen A. Norris

24. Biolistic DNA Vaccination Against Melanoma Julia Steitz and Thomas Tüting

25. Biolistic DNA Vaccination Against Cervical Cancer Michal Šmahel

26. Efficiency of Biolistic DNA Vaccination in Experimental Type I Allergy Verena Raker, Joachim Maxeiner, Angelika B. Reske-Kunz, and Stephan Sudowe

27. Safety Assessment of Biolistic DNA Vaccination Barbara Langer, Matthias Renner, Jürgen Scherer, Silke Schüle, and Klaus Cichutek

Part VI: Related Applications for Biolistic Delivery of Molecules

28. DiOlistics: Delivery of Fluorescent Dyes into Cells Nyssa Sherazee and Veronica A. Alvarez

29. Protein Antigen Delivery by Gene Gun-Mediated Epidermal Antigen Incorporation (EAI) Sandra Scheiblhofer, Uwe Ritter, Josef Thalhamer, and Richard Weiss

Eric J. Yager, Cristy Stagnar, Ragisha Gopalakrishnan, James T. Fuller, and Deborah H. Fuller

20. Methods for Monitoring Gene Gun-Induced HBV- and HCV-Specific Immune Responses in Mouse Models Gustaf Ahlén, Matti Sällberg, and Lars Frelin

21. Gene Gun Immunization to Combat Malaria Elke S. Bergmann-Leitner and Wolfgang W. Leitner

22. Identification of T-cell Epitopes of Mycobacterium tuberculosis with Biolistic DNA Vaccination Toshi Nagata and Yukio Koide

23. Biolistic DNA Vaccination Against Trypanosoma Infection Marianne Bryan, Siobhan Guyach, and Karen A. Norris

24. Biolistic DNA Vaccination Against Melanoma Julia Steitz and Thomas Tüting

25. Biolistic DNA Vaccination Against Cervical Cancer Michal Šmahel

26. Efficiency of Biolistic DNA Vaccination in Experimental Type I Allergy Verena Raker, Joachim Maxeiner, Angelika B. Reske-Kunz, and Stephan Sudowe

27. Safety Assessment of Biolistic DNA Vaccination Barbara Langer, Matthias Renner, Jürgen Scherer, Silke Schüle, and Klaus Cichutek

Part VI: Related Applications for Biolistic Delivery of Molecules

28. DiOlistics: Delivery of Fluorescent Dyes into Cells Nyssa Sherazee and Veronica A. Alvarez

29. Protein Antigen Delivery by Gene Gun-Mediated Epidermal Antigen Incorporation (EAI) Sandra Scheiblhofer, Uwe Ritter, Josef Thalhamer, and Richard Weiss

Gustaf Ahlén, Matti Sällberg, and Lars Frelin

21. Gene Gun Immunization to Combat Malaria Elke S. Bergmann-Leitner and Wolfgang W. Leitner

22. Identification of T-cell Epitopes of Mycobacterium tuberculosis with Biolistic DNA Vaccination Toshi Nagata and Yukio Koide

23. Biolistic DNA Vaccination Against Trypanosoma Infection Marianne Bryan, Siobhan Guyach, and Karen A. Norris

24. Biolistic DNA Vaccination Against Melanoma Julia Steitz and Thomas Tüting

25. Biolistic DNA Vaccination Against Cervical Cancer Michal Šmahel

26. Efficiency of Biolistic DNA Vaccination in Experimental Type I Allergy Verena Raker, Joachim Maxeiner, Angelika B. Reske-Kunz, and Stephan Sudowe

27. Safety Assessment of Biolistic DNA Vaccination Barbara Langer, Matthias Renner, Jürgen Scherer, Silke Schüle, and Klaus Cichutek

Part VI: Related Applications for Biolistic Delivery of Molecules

28. DiOlistics: Delivery of Fluorescent Dyes into Cells Nyssa Sherazee and Veronica A. Alvarez

29. Protein Antigen Delivery by Gene Gun-Mediated Epidermal Antigen Incorporation (EAI) Sandra Scheiblhofer, Uwe Ritter, Josef Thalhamer, and Richard Weiss

Elke S. Bergmann-Leitner and Wolfgang W. Leitner

22. Identification of T-cell Epitopes of Mycobacterium tuberculosis with Biolistic DNA Vaccination Toshi Nagata and Yukio Koide

23. Biolistic DNA Vaccination Against Trypanosoma Infection Marianne Bryan, Siobhan Guyach, and Karen A. Norris

24. Biolistic DNA Vaccination Against Melanoma Julia Steitz and Thomas Tüting

25. Biolistic DNA Vaccination Against Cervical Cancer Michal Šmahel

26. Efficiency of Biolistic DNA Vaccination in Experimental Type I Allergy Verena Raker, Joachim Maxeiner, Angelika B. Reske-Kunz, and Stephan Sudowe

27. Safety Assessment of Biolistic DNA Vaccination Barbara Langer, Matthias Renner, Jürgen Scherer, Silke Schüle, and Klaus Cichutek

Part VI: Related Applications for Biolistic Delivery of Molecules

28. DiOlistics: Delivery of Fluorescent Dyes into Cells Nyssa Sherazee and Veronica A. Alvarez

29. Protein Antigen Delivery by Gene Gun-Mediated Epidermal Antigen Incorporation (EAI) Sandra Scheiblhofer, Uwe Ritter, Josef Thalhamer, and Richard Weiss

Toshi Nagata and Yukio Koide

23. Biolistic DNA Vaccination Against Trypanosoma Infection Marianne Bryan, Siobhan Guyach, and Karen A. Norris

24. Biolistic DNA Vaccination Against Melanoma Julia Steitz and Thomas Tüting

25. Biolistic DNA Vaccination Against Cervical Cancer Michal Šmahel

26. Efficiency of Biolistic DNA Vaccination in Experimental Type I Allergy Verena Raker, Joachim Maxeiner, Angelika B. Reske-Kunz, and Stephan Sudowe

27. Safety Assessment of Biolistic DNA Vaccination Barbara Langer, Matthias Renner, Jürgen Scherer, Silke Schüle, and Klaus Cichutek

Part VI: Related Applications for Biolistic Delivery of Molecules

28. DiOlistics: Delivery of Fluorescent Dyes into Cells Nyssa Sherazee and Veronica A. Alvarez

29. Protein Antigen Delivery by Gene Gun-Mediated Epidermal Antigen Incorporation (EAI) Sandra Scheiblhofer, Uwe Ritter, Josef Thalhamer, and Richard Weiss

Marianne Bryan, Siobhan Guyach, and Karen A. Norris

24. Biolistic DNA Vaccination Against Melanoma Julia Steitz and Thomas Tüting

25. Biolistic DNA Vaccination Against Cervical Cancer Michal Šmahel

26. Efficiency of Biolistic DNA Vaccination in Experimental Type I Allergy Verena Raker, Joachim Maxeiner, Angelika B. Reske-Kunz, and Stephan Sudowe

27. Safety Assessment of Biolistic DNA Vaccination Barbara Langer, Matthias Renner, Jürgen Scherer, Silke Schüle, and Klaus Cichutek

Part VI: Related Applications for Biolistic Delivery of Molecules

28. DiOlistics: Delivery of Fluorescent Dyes into Cells Nyssa Sherazee and Veronica A. Alvarez

29. Protein Antigen Delivery by Gene Gun-Mediated Epidermal Antigen Incorporation (EAI) Sandra Scheiblhofer, Uwe Ritter, Josef Thalhamer, and Richard Weiss

Julia Steitz and Thomas Tüting

25. Biolistic DNA Vaccination Against Cervical Cancer Michal Šmahel

26. Efficiency of Biolistic DNA Vaccination in Experimental Type I Allergy Verena Raker, Joachim Maxeiner, Angelika B. Reske-Kunz, and Stephan Sudowe

27. Safety Assessment of Biolistic DNA Vaccination Barbara Langer, Matthias Renner, Jürgen Scherer, Silke Schüle, and Klaus Cichutek

Part VI: Related Applications for Biolistic Delivery of Molecules

28. DiOlistics: Delivery of Fluorescent Dyes into Cells Nyssa Sherazee and Veronica A. Alvarez

29. Protein Antigen Delivery by Gene Gun-Mediated Epidermal Antigen Incorporation (EAI) Sandra Scheiblhofer, Uwe Ritter, Josef Thalhamer, and Richard Weiss

Michal Šmahel

26. Efficiency of Biolistic DNA Vaccination in Experimental Type I Allergy Verena Raker, Joachim Maxeiner, Angelika B. Reske-Kunz, and Stephan Sudowe

27. Safety Assessment of Biolistic DNA Vaccination Barbara Langer, Matthias Renner, Jürgen Scherer, Silke Schüle, and Klaus Cichutek

Part VI: Related Applications for Biolistic Delivery of Molecules

28. DiOlistics: Delivery of Fluorescent Dyes into Cells Nyssa Sherazee and Veronica A. Alvarez

29. Protein Antigen Delivery by Gene Gun-Mediated Epidermal Antigen Incorporation (EAI) Sandra Scheiblhofer, Uwe Ritter, Josef Thalhamer, and Richard Weiss

Verena Raker, Joachim Maxeiner, Angelika B. Reske-Kunz, and Stephan Sudowe

27. Safety Assessment of Biolistic DNA Vaccination Barbara Langer, Matthias Renner, Jürgen Scherer, Silke Schüle, and Klaus Cichutek

Part VI: Related Applications for Biolistic Delivery of Molecules

28. DiOlistics: Delivery of Fluorescent Dyes into Cells Nyssa Sherazee and Veronica A. Alvarez

29. Protein Antigen Delivery by Gene Gun-Mediated Epidermal Antigen Incorporation (EAI) Sandra Scheiblhofer, Uwe Ritter, Josef Thalhamer, and Richard Weiss

Barbara Langer, Matthias Renner, Jürgen Scherer, Silke Schüle, and Klaus Cichutek

Part VI: Related Applications for Biolistic Delivery of Molecules

28. DiOlistics: Delivery of Fluorescent Dyes into Cells Nyssa Sherazee and Veronica A. Alvarez

29. Protein Antigen Delivery by Gene Gun-Mediated Epidermal Antigen Incorporation (EAI) Sandra Scheiblhofer, Uwe Ritter, Josef Thalhamer, and Richard Weiss

Nyssa Sherazee and Veronica A. Alvarez

29. Protein Antigen Delivery by Gene Gun-Mediated Epidermal Antigen Incorporation (EAI) Sandra Scheiblhofer, Uwe Ritter, Josef Thalhamer, and Richard Weiss

Sandra Scheiblhofer, Uwe Ritter, Josef Thalhamer, and Richard Weiss

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